结晶紫印迹材料的制备及其在鲤鱼样品分析中的应用

以硅胶为基质,结晶紫为模板分子,甲基丙烯酸(MAA)为功能单位,N,N-亚甲基丙烯酰胺(MBA)为交联剂,硅胶为载体,采用表面修饰分子印迹法,制备对结晶紫具有特定亲和选择性的分子印迹聚合物。应用红外光谱对印迹聚合物进行表征,通过静态吸附实验优化实验条件,对聚合物的吸附行为进行分析,采用平衡结合实验评价了分子印迹聚合物的吸附性能。结果表明,采用表面修饰分子印迹法所制备的印迹聚合物对结晶紫表现出较好的特异吸附,对模板分子最大吸附量值为22. 04 mg/g。     

硅胶表面罗丹明B分子印迹聚合物的制备及其性能研究

以表面接枝乙烯基的硅胶为载体,罗丹明B为模板分子,丙烯酰胺为功能单体,乙二醇二甲基丙烯酸酯为交联剂,制备了表面印迹聚合物微球。通过静态吸附对吸附性能进行分析。结果表明,该印迹聚合物对模板分子具有特异吸附性能,并将其应用于固相萃取,测得罗丹明B的加标回收率为94.4%~101.2%,相对标准偏差(n=3)的范围为2.4%~3.4%。     

白杨素分子印迹聚合物的制备及其性能评价

以白杨素为模板分子,硅胶为载体,丙烯酰胺为功能单体,采用表面印迹法制备白杨素分子印迹聚合物。并通过红外光谱测定,吸附实验等对印迹聚合物进行表征及性能评价。静态吸附实验表明,印迹聚合物对模板分子的吸附量远远高于非印迹聚合物;印迹聚合物及非印迹聚合物对白杨素、氯霉素、甲砜霉素的选择吸附表明,所制备的分子印迹聚合物对白杨素有特异性吸附效果。     

苏丹红分子印迹聚合物的合成及吸附性能研究

以苏丹红为模板分子,丙烯酰胺为功能单体,乙二醇二甲基丙烯酸酯为交联剂,偶氮二异丁腈为引发剂,在乙腈中采用沉淀聚合法制备分子印迹聚合物。研究了模板分子与功能单体的相互作用和最佳比例,探讨了超声波/离心提取工艺对分子印迹聚合物洗脱的影响,对合成的分子印迹聚合物进行静态吸附实验、吸附动力学实验以及Scatchard分析。结果表明,在相同条件下,与非印迹聚合物相比,分子印迹聚合物对苏丹红有良好的吸附性能。     

硅胶表面接枝引发转移终止剂制备牛血红蛋白分子印迹聚合物及性能评价

以牛血红蛋白为模板分子,丙烯酰胺为功能单体,N - N甲基双丙烯酰胺为交联剂,通过固定在硅胶表面的引发转移终止剂二乙基二硫代氨基甲酸钠引发聚合反应,应用表面印迹法制备了牛血红蛋白(BHb)印迹聚合物.通过平衡吸附和选择性实验进行评价,结果表明印迹聚合物具有高吸附效率和选择性.     

槲皮素表面分子印迹聚合物的合成及性能研究

以槲皮素为模板分子、硅胶为表面载体、丙烯酰胺为功能单体、乙二醇二甲基丙烯酸酯为交联剂,采用沉淀聚合法制备槲皮素表面分子印迹聚合物。扫描电镜观察聚合物形貌,表明印迹空穴的存在。等温吸附及洗脱实验证明所得表面印迹材料具备较好的特异性吸附性能,当吸附液浓度为3 mg/mL时,印迹材料对槲皮素的吸附量可以达到31.9 mg/g,本研究可为天然药物中黄酮类槲皮素成分的提取分离提供新思路与新方法。     

三硝基甲苯分子印迹微球的制备与应用

以三硝基甲苯（TNT）为模板分子,丙烯酰胺（AM）为功能单体,采用沉淀聚合法制备分子印迹聚合物MIP。通过紫外光谱法研究TNT和AM之间的相互作用,结果表明TNT与AM之间作用力较强,有助于形成结构稳定、亲和性强的聚合物;利用扫描电镜观测不同制备条件下印迹聚合物的表观形貌,发现乙腈用量为100mL,缓慢的搅拌速率下制备的聚合物形貌较优异;利用红外光谱分析聚合物的结构特征,验证印迹聚合物的制备与模板分子的洗脱;平衡吸附实验表明,MIP对TNT的吸附存在两种作用位点并且吸附量随TNT初始浓度增加而增加;动力学吸附实验发现印迹聚合物对TNT的吸附速率远大于非印迹聚合物;选择性吸附实验中,MIP对TNT表现出较好的特异吸附性能,而对于TNT的结构类似物DNT、RDX吸附能力较差。     

核壳型磁性表面分子印迹聚合物的制备及对罗丹明6G吸附性能研究

以罗丹明6G为模板分子,多巴胺和3-氨基苯硼酸为功能单体和交联剂,采用表面分子印迹技术,首次制备了对罗丹明6G具有优良吸附作用的核壳型磁性表面分子印迹聚合物。应用扫描电镜、透射电镜、红外光谱等对其进行表征,通过静态、动态和选择性吸附实验,研究了该分子印迹聚合物的识别选择性和吸附容量,并建立了固相萃取-分光光度法测定实际样品中痕量罗丹明6G的新方法,检测限为2.7nmol/L,回收率为86.9%~105.0%。     

N - BOC -L-色氨酸分子印迹聚合物的手性拆分

以分散聚合法制备的聚苯乙烯微球为种子,采用单步溶胀悬浮聚合法,N - BOC -L -色氨酸为模板分子,丙烯酰胺为功能单体,乙二醇二甲基丙烯酸酯为交联剂,在水相中成功制备了分子印迹聚合物微球,吸附实验表明,印迹聚合物对模板N - BOC -L-色氨酸有良好的吸附性,该聚合物作为液谱固定相,实现了模板与其对映体的快速基线...     

木犀草素温敏分子印迹聚合物的合成及性能研究

以木犀草素为模板分子、丙烯酰胺为功能单体、N,N-亚甲基双丙烯酰胺为交联剂、N-异丙基丙烯酰胺为温敏单体,采用沉淀聚合法制备木犀草素温敏分子印迹聚合物。扫描电镜观察聚合物形貌,表明印迹空穴的存在。不同温度下吸附及洗脱实验证明所得印迹材料具备较好的特异性吸附性能及温度响应行为,可为天然药物中黄酮类木犀草素成分的提取分离提供新思路与新方法。     

异丙酚分子印迹聚合物的制备及其结合特性

采用分子印迹技术制备了异丙酚分子印迹聚合物,并考察其结合特性和选择性。以异丙酚为模板分子,甲基丙烯酸为功能单体,二甲基丙烯酸乙二醇酯为交联剂,偶氮二异丁腈为引发剂,甲苯、正己烷为溶剂合成异丙酚分子印迹聚合物。通过平衡吸附实验,研究了异丙酚分子印迹聚合物的结合特性和选择性。Scatchard模型分析表明,在该聚合物中存在对异丙酚有不同亲和力的两类作用位点。此聚合物与异丙酚的结合要优于麝香草酚。此方法可合成异丙酚分子印迹聚合物,通过结合实验和对底物选择性考察,表明该聚合物对异丙酚具有良好的选择性。     

奎宁印迹聚合物的吸附与识别特性的研究

以奎宁为模板分子 ,利用分子印迹技术合成了一种新型的类似于人工受体的印迹聚合物 ,研究了它对奎宁和其它底物的吸附特性和选择性识别能力 ,并对其固相萃取性能做了初步的研究。     

克伦特罗分子印迹聚合物吸附性能研究

选用分子印迹技术,以克伦特罗为印迹分子,α-甲基丙烯酸为功能单体,乙二醇二甲基丙烯酸酯为交联剂,合成克伦特罗分子印迹聚合物,研究其吸附性能。结果表明,与组成相同的非印迹聚合物相比,克伦特罗印迹聚合物有较大的吸附性能,有望在克伦特罗分离分析中作为固相萃取的材料。     

TiO_2/ACF复合材料的制备及表征

采用浸渍-提拉法和溶胶-凝胶法制备TiO2/ACF复合光催化材料,用BET、XRD和SEM等进行表征。结果表明,溶胶-凝胶法制备的复合材料由于负载了大量TiO2,其比表面积明显低于浸渍法;浸渍法制备的复合材料随着粘结剂添加量的增加,比表面积呈减小趋势。溶胶-凝胶法制备的复合材料的表面TiO2锐钛晶型较明显,浸渍-提拉法无明显的锐钛晶型。采用浸渍-提拉法制备的TiO2/ACF复合材料,TiO2以粒状负载于ACF表面;溶胶-凝胶法中TiO2在ACF表面形成一层薄膜,由于焙烧,薄膜产生裂隙,暴露出ACF,有利于吸附和催化作用的同时进行。     

用于蛋白质BSA识别的温度/pH双敏印迹聚合物

用N-异丙基丙烯酰胺(NIPAM)和壳聚糖为功能单体,牛血清白蛋白（BSA）为模板蛋白,在改性SiO₂表面制备温度/pH双敏蛋白质印迹聚合物。TEM、FTIR和TG等结果证明印迹层已成功接枝在载体表面。系统研究了聚合物的温度/pH双敏性、吸附容量、吸附动力学、特异性、竞争吸附性及重复性。结果表明,印迹聚合物（MIP）的溶胀率和吸附容量受温度和pH影响较大,高温碱性收缩,低温酸性溶胀。在pH 4.6和35℃下,对0.6mg/mL BSA吸附4h时获得较大的吸附容量（83.74mg/g）,印迹因子为2.02。同时MIP也有较好的特异性和竞争吸附性。重复5次后,吸附容量仍能维持在88%,说明重复性良好。这种新型的温度/pH双敏蛋白质分子印迹合成方法简单,在蛋白质的分离和识别方面有较好的应用前景。     

分子印迹技术手性分离氨基酸衍生物(Ⅰ)—分子印迹聚合物的制备、色谱评价及物理化学表征

利用分子印迹技术制备了对L 特丁氧羰基色氨酸或D 特丁氧羰基色氨酸印迹的丙烯酰胺分子印迹聚合物。色谱评价结果表明,印迹分子L Boc Trp的离解常数(3 287mmol/L)要小于非印迹分子D Boc Trp的离解常数(4 379mmol/L),说明分子印迹聚合物对印迹分子具有特异亲和作用。采用扫描电镜和红外光谱等物理化学表征技术从结构上对这种特异性作了进一步的分析。结果表明,分子印迹聚合物是一种具有多层次孔结构的聚合物,为溶质的扩散提供了良好的通道;在聚合物表面存在可与印迹分子相互作用的官能团。     

Molecularly imprinted polymers synthesized via semi-covalent imprinting with sacrificial spacer for imprinting phenols

Semi-covalent imprinting with carbonyl group as sacrificial spacer was employed to synthesize molecularly imprinted polymer (MIP) for phenols. A series of semi-covalently imprinted polymers were prepared by varying the templates and porogens. The MIP with 4-chlorophenyl (4-vinyl)phenyl carbonate as template was proved to be the best one, with ethylene glycol dimethacrylate (EGDMA) as cross-linker, 2,2-azobisisobutyronitrile(AIBN), and chloroform as initiator and porogen, respectively. Under such conditions, the corresponding non-covalently imprinted polymer was fabricated with 4-chlorophenol (4-CP) as template and 4-vinylpyridine (4-VP) as functional monomer. The polymer prepared by semi-covalent imprinting displayed superior selectivity to the non-covalently imprinted polymer for phenols. The peak broadening and tailing had been largely reduced on the column packed with semi-covalently imprinted polymer. Meanwhile, the constant retention for these phenols and the good linearity for phenol and 4-CP augured that the semi-covalently imprinted polymer had the potential application as stationary phase for quantitative determination of phenols.     

Preparation and Properties of Uniform-Sized Polymer Beads Imprinted with N-CBZ-L-Phenylalanine

ABSTRACT

A series of uniform-sized polymer beads molecularly imprinted for N-carbobenzoxy-L-phenylalanine (N-Cbz-L-Phe) were prepared by a two-step swelling and suspension polymerization method using polystyrene latex particles as precursors, 2-vinypyridine and α-methylacrylamide as host functional monomers and diethylene glycol diacrylate as crosslinker. The uniformly sized polystyrene precursors were synthesized by emulsifier-free emulsion polymerization. The obtained materials had an obvious imprinting effect to the template N-Cbz-L-phenylalanine. The imprinted polymer also had some adsorption to the molecules, which were similar to the template molecule configuration. The adsorbing dynamics of the polymer to the template molecule, and the effects of crosslinking degree of the polymer and the environmental temperature on the absorbing properties were also discussed.     

The Preparation of BHb-Molecularly Imprinted Gel Polymers and Its Selectivity Comparison to BHb and BSA

Molecularly imprinted gel polymer (MIP) for the selective imprinting of bovine hemoglobin (BHb) was prepared in aqueous media by bulk polymerization using polyacrylamide matrix. The synthesis conditions of BHb-MIP were investigated, which involved the interaction of functional monomers and template protein in different molar ratio, solution pH, and ionic strength. The adsorption experiments indicated that BHb-MIP had a high affinity for BHb over the non-imprinted polymers. The selectivity of BHb-MIP for BHb and bovine serum albumin (BSA) with similar molecular weight was compared. It was demonstrated that BHb-MIP had better selective adsorption and recognition properties to BHb especially in the presence of BSA as competing protein. It might be helpful for selectively separating template protein with MIP from the proteins mixture with similar molecular weight.     

Metal ion imprinted microsphere prepared by surface molecular imprinting technique using water-in-oil-in-water emulsions

A novel metal ion imprinted polymer was prepared by the surface molecular imprinting technique. Trimethylolpropane trimethacrylate (TRIM), zinc ions, and 1,12-dodecanediol-O,O′-diphenyl phosphonic acid (DDDPA) were used as the matrix-forming monomer, imprint molecule, and functional monomer, respectively. We have prepared Zn(II)-imprinted microspheres utilizing water-in-oil-in-water emulsions, which are spherically well defined and uniform. TRIM comprises of three polymerizable groups that serve to align the recognition sites produced on the polymer surface with better template effect. We conducted diagnostic zinc adsorption tests by using the Zn(II)-imprinted and unimprinted polymers in order to make an assessment on the effectiveness of TRIM-based and divinylbenzene-based imprinted polymers. The template effect of the TRIM-based polymer was enhanced by the high rigidity of the polymer matrix compared to that of the imprinted polymer whose matrix is divinylbenzene. In addition, the adsorption equilibrium constant was evaluated on the basis of the Langmuir analysis for the adsorption data. The method to prepare metal-imprinted microspheres on the surface of polymers crosslinked by TRIM is easy, and the adsorbent produced can be readily used without subsequent grinding or sieving. © 1999 John Wiley & Sons, Inc. J Appl Polym Sci 73: 1223–1230, 1999