不孕妇女宫颈粘液及血清中抗精子抗体的检测及其临床意义

为探讨宫颈粘液（ＣＭ）与血清中抗精子抗体（ＡｓＡｂ）的关系，评价检测ＣＭ中ＡｓＡｂ的临床意义，检测了１９例不明原因不孕患者及１４例对照组妇女ＣＭ及血清中的ＡｓＡｂ。结果：不孕组ＣＭ中ＡｓＡｂ阳性率（２１．０５％）明显高于对照组（７．１４％），Ｐ〈０．０５。血清中两且间无显著差异，ＣＭ与血清中ＡｓＡｂ的检出率无明显相关性，提示对于不有原因不孕妇女，尤其是宫颈性不孕，检测ＣＭ中的ＡｓＡｂ比检测血清中的     

抗精子抗体和抗子宫内膜抗体与不孕及流产的关系

目的　探讨抗精子抗体（ＡｓＡｂ）与抗子宫内膜抗体（ＥＭＡｂ）在不孕、流产中的作用及二者关系,进一步 揭示不孕、流产的病因。方法　采用酶联免疫吸附试验（ＥＬＩＳＡ）同时检测３４５例不孕、流产妇女血清中的ＡｓＡｂ与 ＥＭＡｂ,按ＡｓＡｂ检测结果分阳性组和阴性组,比较两组ＥＭＡｂ阳性率的差异。结果　原发不孕及自然流产妇女中, ＡｓＡｂ（－）组ＥＭＡｂ阳性率高达１６．６７％和１９．５１％,而ＡｓＡｂ（＋）组ＥＭＡｂ阳性率高达４３．６４％和４２．８６％,显著高于 ＡｓＡｂ（－）组（Ｐ＜０．０１,Ｐ＜０．０５）。继发不孕妇女中ＡｓＡｂ（－）组ＥＭＡｂ阳性率为１８．１８％,ＡｓＡｂ（＋）组ＥＭＡｂ阳性率 为３２．２％,二者无显著性差异（０．０１＞Ｐ＞０．０５）。结论原发不孕及自然流产妇女中因个体免疫反应差异使某些 人易对体内、外物质发生免疫反应而产生抗体,从而导致不孕或流产。     

抗精子抗体与慢性前列腺炎的相关性研究

目的：探讨抗精子抗体（ＡｓＡｂ）与慢性前列腺炎的关系。方法：采用酶联免疫吸附试验（ＥＬＩＳＡ）检测慢性前列腺炎不育男性、非慢性前列腺炎不育男性及正常生育男性的血清及精浆中的ＡｓＡｂ。结果：慢性前列腺炎不育组ＡｓＡｂ阳性率为５４．６％,非慢性前列腺炎不育组ＡｓＡｂ阳性率明显高于非慢性前列腺炎不育组及正常生育组（Ｐ〈０．０５,Ｐ〈０．０１）;慢性前列腺炎患者经抗炎治疗后,治愈组ＡｓＡｂ阳性率为１７．４     

宫颈糜烂与抗精子抗体相关性的研究

采用酶联免疫吸附试验检测了不孕伴宫颈糜烂，单纯不孕及正常组的血清和宫颈粘液中的抗精子抗体。结果显示：不孕伴宫颈糜烂组ＡｓＡｂ阳性率为６７％，其中血清ＡｓＡｂ阳性占１５％，宫颈粘液ＡｓＡｂ阳性占５２％，单纯不孕组ＡｓＡｂ阳性率为２７％，其中血清ＡｓＡｂ阳性中２０％，宫颈粘液ＡｓＡｂ阳性占７％；正常组ＡｓＡｂ阳性率为８％。３组相比均有显著性差异。     

不孕妇女衣原体及支原体感染对性交后试验的影响

目的：探讨不孕妇女生殖道沙眼衣原体（ＣＴ）及解脲支原体（ＵＵ）感染对性产后的试验（ＰＣＲ）的影响。方法：本研究用ＰＣＲ方法检测了２０３例不孕妇女性交后宫颈粘液分泌物中ＣＴ及ＵＵ－ＤＮＡ表达。结果：ＰＣＲ不良组ＣＴ及ＵＵ－ＤＮＡ阳性率分别为３０．３％和２８．３％,显著高于ＰＣＴ正常组（Ｐ〈０．０５）。结论：ＣＴ及ＵＵ感染改变宫颈粘液性状,阻止精子从宫颈穿过是导致不孕的原因之一。     

Graves患者TSH抗独特型抗体检测及其意义

根据自身免疫性甲状腺疾病发病机制，新的独特型－抗独特型免疫网络学说，用兔抗人ＴＳＨＡｂ检测ＴＳＨＡｂ_２。以正常人为对照，其结合率ｘ＋２ｓ为正常上限，大于此值为阳性。１００例ＴＲＡｂ阳性组有５２％患者ＴＳＨＡｂ_２阳性，５０例ＴＲＡｂ阴性组有４％患者ＴＳＨＡｂ２_阳性，两组有明显差异（Ｐ＜０．０５）。ＴＲＡｂ和ＴＳＨＡｂ_２正相关（ｒ＝０．５８２，Ｐ＜０．０５）。同时用制备的ＴＳＨ抗体吸附ＴＲＡｂ阳性血清后再测ＴＲＡｂ吸附前后有明显差异（Ｐ＜０．０５）。ＴＳＨＡｂ_２的检出为探讨Ｇｒａｖｅｓ’发病机制提供了一条新思路。     

人工流产后不孕患者抗精子抗体监测

采用酶联免疫吸附试验（ＥＬＩＳＡ），对７８例因人工流产后所引起的不孕症、１４０例非人工流产不孕症以及２８例正常已婚育龄妇女进行血清抗精子抗体检测。结果：人工流产后不孕组、非人工流产不孕组及正常组抗精子抗体的阳性率分别为３３．３３％，１５．７１％，３．５７％。人工流产后不孕组血清中抗精子抗体的阳性率显著高于非人工流产不孕组和正常已婚育龄妇女（Ｐ＜０．０５）。提示：由人工流产后所引起的不孕症，在某些方面可能与免疫因素有关。子宫内膜的破损和炎症可能是抗精子抗体产生的一个重要诱因。     

抗精子抗体和抗子宫内膜抗体与不孕及流产的关系

目的 探讨抗精子抗体（ＡｓＡｂ）与抗子宫内膜抗体（ＥＭＡｂ）知不孕、流产中的作用及二者关系,进一步揭示不孕、流产的病因。方法 采用酶联免疫吸附试验（ＥＬＩＳＡ）同时检测３４５例不孕、流产妇女血清的ＡｓＡｂ与ＥＭＡｂ,按ＡｓＡｂ检测结果发阳性组和阴性组,比较两组ＥＭＡｂ阳性率的差异。结果 原发不孕及自然流产妇女中,ＡｓＡｂ（－）组ＥＭＡｂ阳性率高达１６．６７％和１９．５１％,而ＡｓＡｂ（＋）组ＥＭＡ     

不孕妇女宫颈粘液和血清抗精子抗体测定

本文检测１５０例不孕妇女宫颈粘液和血清抗精子抗体。宫颈粘液检出２６例ＩｇＡ类抗精子抗体，３２例ＩｇＧ类抗精子抗体，其中有１０例重叠阳性，总阳性率为３２％（４８／１５０）；血清检出抗精子抗体３０例，血精与宫颈粘液有２０例重叠阳性，总阳性率为３８．７％（５８／１５０）；因此同时检测不孕妇女的血清和宫颈粘液能提高免疫性不孕症的检出率。     

流式细胞仪检测胃癌中CD44s及CD44v6表达

目的　探讨黏附因子ＣＤ４４ｓ和ＣＤ４４ｖ６的表达与胃癌生物学行为的关系。方法　应用流式细胞术对５８例胃癌及４０例胃良性病变的新鲜标本检测ＣＤ４４ｓ和ＣＤ４４ｖ６,并应用免疫组化测定ＰＣＮＡＬ１。结果　胃癌组ＣＤ４４ｓ的阳性率（６２．１％,３６／５８）明显高于良性病变组（２７．５％,１１／４０,Ｐ＜０．０１）,且与ＰＣＮＡＬＩ（Ｐ＜０．０５）有关。胃癌组ＣＤ４４ｖ６的阳性率（５８．６％,３４／５８）高于良性病变组（３２．５％,１３／４０,Ｐ＜０．０５）,且与肿瘤部位（Ｐ＜０．０５）、淋巴结转移（Ｐ＜０．０５）及ＰＣＮＡＬＩ（Ｐ＜０．０５）有关。ＣＤ４４ｓ与ＣＤ４４ｖ６表达在胃良性病变组（Ｒｓ＝０．５９,Ｐ＜０．０１）及胃癌组中（Ｒｓ＝０．２９,Ｐ＜０．０５）存在相关性。结论　ＣＤ４４ｓ和ＣＤ４４ｖ６之间存在相关性并在胃癌中表达增强并和增殖活性有关,ＣＤ４４ｖ６与肿瘤部位及淋巴结转移有关。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

Development on Adjustable Calibration Marker for Shock Wave Focus

Shock wave lithotripsy （SWL） is a treatment of choice for upper urinary stones. However, this procedure is inappropriate for obese patients because the focus is often unable to reach the target owing to the limited focal distance in shock wave source. Although treating such patients in a blast path may increase the application length of shock wave source, it＇s difficult to find this path on the lithotripter monitor. For this reason, we invented an adjustable calibration marker in order to set an effective focus in the shock wave hath.     

Study on the TCM Pathogenesis of Angiopathic Complications of Type II Diabetes

Excess production of reactive oxygen species(ROS)of mitochondrion mediated by hyperglycemia is the common pathogenesis of angiopathic complications of diabetes.TCM holds that the damp from the dysfunction of spleen.kidney and liver is the causative factor of complications of diabetes.This is similar to the mechanism of Ros resulting in angiopathic complications of diabetes.When the angiopathic complications of type II diabetes mellitus(T2DM)are difierentiated as caused by turbid damp in TCM can be explained as ROS.Since the obstruction of pathogenic damp in channels and collaterals is said to be the main pathogenesis,the treating principle should be dissolving the damp to remove the obstruction.     

Leptin, Ghrelin and TNF-α before and after Hypo-caloric Traditional Chinese Diet and Auricular Acupuncture

INTRODUCTION Obesity is a complex emergent problem, which can be possibly solved not only by the diet but also by the life style and promotion of a constant physical exercise. 1, 2 No doubt careful attentions must be given to the nutritional condition of obese people, the dietary habits, the somatic build (i.e. distribution of fat mass) and the organic functions linked to formation of the fat mass. All the parameters should be constantly monitored before, during and after a diet treatment. 3, 4, 5     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Relationship between Dysglycemia and Carotid Atherosclerosis in Tibetan Population

People with dysglycemia are at high risk for atherosclerotic diseases. This study aims at investigating the atherosclerotic vascular damage in dysglycemia and its metabolic origin in Tibetan population.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

CLINICAL SIGNIFICANCE OF SERUM CYTOKINES IL-1β,sIL-2R,IL-6,TNF-α,AND IFN-v IN ACUTE CORONARY SYNDROME

Objectives To explore serum cytokines levels (including IL-1 β, sIL-2R, IL-6, TNF-α, and IFN-v) and their significance in patients with acute coronary syndrome (ACS) and the subsequent follow-ups, with attempt to estimate the role of various serum inflammatory markers in the diagnosis and assessment of ACS.Methods The study population include 40 patients with acute myocardial infarction (AMI), 40 patients with unstable angina pectoris (UAP), and 40 controls. Among the 80 patients, 60 patients attended a follow up 4 months later. Serum inflammatory markers including IL-1 β, sIL-2R, IL-6, TNF-α, and IFN-v were measured by enzyme linked immunosorbent assay.Results Serum IL- 1 β, sIL-2R, IL-6, TNF-α were significantly higher in AMI group or UAP group compared to the control group and became significantly lower 4 months later in the follow-up patients. Serum levels of IFN-v shows no significant difference between AMI group or UAP group and controls, also showing no significant change when measured in follow up patients. There was no correlation between serum creatine kinase-MB isoenzyme levels and serum inflammatory markers either in UAP or AMI group. Furthermore, when divided into two subgroups using Wagner's QRS scoring system in the AMI group, there is no difference of each serum inflammatory marker between ≤ 6 scores group and ＞ 6 scores group.Conclusion Serum levels of certain inflammatory markers may have some diagnostic value for ACS, and can be a useful marker reflecting disease stability.     

Possible Spread of adenovirus type 3 from poultry to humans ：indirect evidence from an outbreak in China

Objective:To explore the epidemiology and etiology for an outbreak of acute respiratory tract infection that occurred in one county of Jiangsu Province, China 2004. Methods: Only cases meeting the case definition were included in the study. We reviewed the medical records of the cases who were admitted to the local hospitals, interviewed cases by a standard questionnaire, and then described the epidemiotogic features and analyzed risk factors by means of a case-control study. We collected pharyngeal swab specimens and sent them to different laboratories for isolation and culture. The laboratory used different detection methods such as DIP, PCR, electron microscope examination and microneutralization assay, to identify and then type the positive specimens. Results:A total of 871 cases were reported during the period from April 18 to July 4,2004. The distribution of onset times presented two peaks, one in late May and another in middle June. The epidemic occurred mainly in the elementary and junior high schools in ten townships of one county, and the mean age of the cases was 12 years (range 7 months to 18 years). The course of the disease was acute, and was characterized by fever accompanied with sore throat and tonsillitis. The WBC count of cases was normal or elevated. The mean duration of illness was 5 days (range 2 to 12 days). No fatalities from illness were reported. A case-control study indicated that the possible risk factors were close contact with a case and/or poultry before onset and sharing of towels among members of the family. The typical CPE was observed through inoculating pharyngeal swab specimens into the HEP-2 cell cultures in different laboratories. An infection of adenovirus type 3 was verified by detecting positive specimens in different methods. Conclusion:This investigation demonstrated that the acute respiratory infection in cases was caused by adenovirus type 3. Cases occurred in over 70 schools in ten townships in 2004, and the route of transmission was possibly close contact with cases or droplet transmission.