共查询到20条相似文献,搜索用时 218 毫秒
1.
以蔗糖密度梯度离心提取猪皮下脂肪细胞和其他组织细胞膜蛋白,经SDS—PAGE分析,结果表明:皮下脂肪细胞与其他组织细胞膜蛋白的组成存在较大差异,但仍有一些相同的膜蛋白成分;以皮下脂肪细胞膜蛋白为抗原,免疫山羊制备了抗脂肪细胞膜蛋白抗体,经ELISA测定抗体效价迭1:12800,经western世界人民blotting鉴定,所制备的抗体与肾周脂肪、肠系膜脂肪、心、肝、脾、肺、肾、肌肉和红细胞膜蛋白有交叉反应,但反应原性较弱,表明制备出的抗脂肪细胞膜蛋白抗体是高效价的,有一定特异性的。 相似文献
2.
介绍了脂肪细胞膜蛋白及其抗体的研究进展,以及用脂肪细胞膜蛋白抗体免疫动物的作用机制和效果,并阐述了它在动物生产及人的肥胖症等方面的应用前景。 相似文献
3.
介绍了脂肪细胞膜蛋白及其抗体的研究进展,以及用脂肪细胞膜蛋白抗体免疫动物的作用机制和效果,并阐述了它在动物生产及人的肥胖症等方面的应用前景。 相似文献
4.
介绍了脂肪细胞膜蛋白及其抗体的研究进展,以及用脂肪细胞膜蛋白抗体免疫动物的作用机制和效果,并阐述了它在动物生产及人的肥胖症等方面的应用前景. 相似文献
5.
6.
为了研究抗脂肪细胞膜蛋白抗体对猪内脏器官重量及红细胞比容的影响,试验首先制备了兔抗猪脂肪细胞膜蛋白抗体,然后进行动物饲养试验。选择健康、胎次相近、体重为13 ̄15kg的6周龄云南长撒二元杂交仔猪20头,公母各半,随机分为4组,每组5头。Ⅰ、Ⅱ、Ⅲ组为试验组,腹腔内分别注射抗脂肪细胞膜蛋白抗体10、20和30ml;Ⅳ为对照组,注射20ml非免疫血清。研究结果表明:抗脂肪细胞膜蛋白抗体免疫对心脏和肾脏重量有一定影响,而且随剂量的增加而增大;对红细胞比容有一定的影响,但一般2周后恢复正常。 相似文献
7.
本文综述了脂肪细胞膜免疫的种类及其降低动物胴体脂肪的作用机理和效果,阐述了目前存在的问题,并展望了脂肪细胞膜免疫技术的应用前景。 相似文献
8.
动物生产性能的发挥是遗传基因、营养水平、环境条件及神经内分泌调节等诸多因素综合影响的结果。为提高动物的生产性能,人们从生理、营养和饲养管理调控等方面进行了探讨,其中使用生理诃节物质直接和间接地调节动物生理机能,促进动物生产性能的发挥则成为当前研究的热点。许多研究表明,半胱胺、环腺苷酸和脂肪细胞膜蛋白抗体等生理调节物质,对调节动物的生理功能,提高动物生产性能具有较好的促进作用,具有良好开发应用的前景。本文就半胱胺、环腺苷酸和脂肪细胞膜蛋白抗体三种生理调节物质的研发应重点解决的技术问题作一简述。 相似文献
9.
10.
HU Hong-mei ZHU Rong-sheng Institute of Animal Science Veterinary Medicine Shandong Academy of Agricultural Sciences Jinan Shandong LIU Ling-yun Primary Education DePartment of Kunming Normal Junior College Kunming Yunnan GAO Shi-zheng Yunman Provincial Key Lab of Animal Nutrition Feed Kunming Yunnan 《家畜生态》2007,(5)
选择健康、胎次相近、体重为15kg的6周龄云南长撒二元杂交仔猪20头,公母各半,随机分为4组,每组5头。Ⅰ、Ⅱ、Ⅲ组为试验组,于试验开始第一天各组每头猪分别腹腔内免疫抗脂肪细胞膜蛋白抗体10mL、20mL和30mL抗体;、Ⅳ为对照组,注射20mL非免疫血清。研究结果表明:抗脂肪细胞膜蛋白抗体可明显改善猪胴体组成。仔猪阶段(15kg)腹膜内免疫20mL、30mL抗体对猪胴体组成的改善效果最明显,脂肪率分别降低22.97%和22.25%(P<0.01);背膘厚分别减少18.85%和17.91%(P<0.05);板油重和花油重分别减少13.36%、13.70%和11.69%、11.03%(P<0.05);瘦肉率分别提高7.76%和7.62% (P<0.05);眼肌面积分别增加28.03%和24.21%(P<0.01)。 相似文献
11.
12.
13.
魏静 《四川畜牧兽医学院学报》2009,(4):28-32
在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。 相似文献
14.
本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。 相似文献
15.
16.
以国际标准强毒R株人工感染非免疫产蛋鸡,定时扑杀,分别从鼻窦、眶下孔、气管、肺、气囊、卵巢和输卵管分离MG,并收集感染鸡所产蛋分离MG。结果表明,人工感染48小时后上、下呼吸道及肺已被全面感染,96小时气囊已被感染,120小时输卵管已能分离到MG,卵巢始终分离不到MG。人工感染鸡自144小时便能在其所产蛋中分离出MG。药物治疗能在72小时内消除感染,油乳剂苗则需24天后逐渐降低蛋内MG分离率,药物卵内注射、种蛋药浴、高温处理均能杀死卵内MG,但以研制的种蛋浸泡剂药浴效果为最好。 相似文献
17.
REASONS FOR PERFORMING STUDY: Centesis of the bicipital bursa using an 8.9 cm long spinal needle has been reported but the alternative of employing a 3.8 cm long hypodermic needle requires validation. OBJECTIVE: To compare the efficacy of 2 different methods of centesis of the bicipital bursa and to evaluate the usefulness of ultrasonographic imaging to determine the location of solution administered when centesis of the bursa is attempted. METHODS: For Trial 1, 6 clinicians, who had no previous experience of centesis of the bicipital bursa, attempted to inject a solution composed of an aqueous radiopaque contrast medium and physiological saline solution (PSS) into the bicipital bursae of 2/12 horses using the previously described distal approach to inject one bursa and a proximal approach to inject the contralateral bursa. The bicipital tendon and bursa were examined ultrasonographically before and after injection; and both shoulders were examined radiographically to identify the location of the medium. In Trial 2, another 6 clinicians, also with no previous experience of centesis, repeated Trial 1, using 6 horses, but the radiopaque contrast medium was mixed with air instead of PSS. RESULTS: Accuracy of centesis using the proximal approach was 39% and that of the distal approach 28%. Ultrasonographic examination of the shoulder allowed the location of solution and air to be accurately predicted in all 12 shoulders examined. CONCLUSIONS: Clinicians who have had no previous experience performing centesis of the bicipital bursa are unlikely to be successful in centesis using either approach. Radiographic examination after injecting a radiopaque contrast medium may be necessary to assess the success of centesis especially if bursal fluid is not obtained during centesis. Injecting air along with the radiopaque contrast medium provides more accurate ultrasonographic confirmation of centesis and better radiographic definition than does injection without air. 相似文献
18.
19.
20.
Effects of size of ingestively masticated fragments of plant tissues on kinetics of digestion of NDF
Ingestively masticated fragments were collected and sized via sieving. Different sizes of esophageal masticate and ruminal digesta fragments, and ground fragments of larger masticated pieces were incubated in vitro, and undigested NDF remaining at intervals of up to 168 h of incubation was determined. The ruminal age-dependent time delay (tau) for onset of digestion of NDF was positively correlated (P < 0.004) with the mean sieve aperture estimated to retain 50% of the fragments between successive sieve apertures (MRA). Degradation rate of potentially degradable NDF (PDF) and level of indigestible NDF were not related (P > 0.10) to MRA of masticated and ground fragments. Estimates of tau were positively related to MRA, with slopes of bermudagrass < corn silage < ruminal fragments of corn silage. It was concluded that fragment size-, and consequently, ruminal age-dependent onset of PDF degradation of a mixture of different fragment sizes results in an age-dependent rate of degradation of the more rapidly degrading of two subentities of PDF. Models are proposed that assume a tau before onset of simultaneous degradation of PDF from two pools characterized as having gamma-modeled age-dependency and age-constant rates. The ruminal age-dependent pool seems to be associated with the faster-degrading pool, and its rate parameter increases with range in MRA in the population of fragments. Conceptually, the ruminal age-dependent rate parameter for PDF degradation seems to represent a composite of several effects: 1) effects of the size-dependent tau; 2) range in MRA of the population of ingestively masticated fragments; and 3) subentities of PDF that degrade via more rapid age-dependent rates compared with subentities of PDF that degrade via age-constant rates. The estimated fractional rates of ruminative comminution of ingestively masticated fragments (0.060 to 0.075/h) were of a magnitude similar to the mean fractional rates of PDF digestion (0.030 to 0.085/h), which implies that ruminative comminution may be first-limiting to fractional rate of PDF digestion. The in vivo roles of ingestive and ruminative mastication of fragments on PDF degradation must be considered in any kinetic system for estimating PDF digestion in the rumen. These results and others in the literature suggest that the rate of surface area exposure rather than intrinsic chemical attributes of PDF may be first-limiting to degradation rate of PDF in vivo. 相似文献