| 2型糖尿病患者miR-15a、miR-16、miR-20b变化及与糖尿病视网膜病变的相关性 |
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| 引用本文： | 刘林平,吴伯乐,倪莉莎,张丽娜,胡夏云,王宗,陈玲.2型糖尿病患者miR-15a、miR-16、miR-20b变化及与糖尿病视网膜病变的相关性[J].中华全科医学,2020,18(6):954-958. |
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| 作者姓名： | 刘林平 吴伯乐 倪莉莎 张丽娜 胡夏云 王宗 陈玲 |
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| 作者单位： | 1. 丽水市人民医院眼科, 浙江 丽水 323000; |
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| 基金项目： | 浙江省医药卫生科技计划项目(2018KY924) |
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| 摘 要： | 目的 探讨2型糖尿病(T2DM)患者微小RNA-15a(miR-15a)、微小RNA-16(miR-16)、微小RNA-20b(miR-20b)变化及其与糖尿病视网膜病变(diabetic retinopathy,DR)关系。 方法 选取2017年5月—2019年5月丽水市人民医院收治的105例T2DM患者,均接受白内障超声乳化手术,根据术后结果将其分为无DR组32例、非增生性糖尿病视网膜病变(non-proliferative diabetic retinopathy,NPDR)组39例、增生性糖尿病视网膜病变(proliferative diabetic retinopathy,PDR)组34例,另选取同期单纯白内障患者40例作为对照组。采用miRNA衍生片段长度多态性(miRFLP)直接测定法检测房水miR-15a、miR-16、miR-20b水平,采用实时荧光定量PCR(qRT-PCR)测定血清miR-15a、miR-16、miR-20b水平,用ROC曲线分析房水和血清中miR-15a、miR-16、miR-20b水平对PDR的预测价值。 结果 房水和血清中miR-15a、miR-16、miR-20b水平由高到低依次为PDR组、NPDR组、无DR组、对照组,两两比较差异均有统计学意义(均P<0.05)。DR患者房水与血清中miR-15a、miR-16、miR-20b水平均呈正相关(均P<0.05)。房水中miR-15a、miR-16、miR-20b水平联合预测PDR的曲线下面积为0.912,敏感度高达91.20%;血清中三者联合预测PDR的曲线下面积为0.931,特异性高达92.30%,预测价值均优于三者单一应用。 结论 T2DM患者房水和血清中miR-15a、miR-16、miR-20b水平升高与DR发生有关。
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| 关 键 词： | 2型糖尿病 微小RNA-15a 微小RNA-16 微小RNA-20b 糖尿病视网膜病变 |
| 收稿时间： | 2019-07-21 |
Changes of miR-15a,miR-16 and miR-20b in patients with type 2 diabetes mellitus and their relationship with diabetic retinopathy |
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| Affiliation: | Department of ophthalmology, Lishui People's Hospital, Lishui, Zhejiang 323000, China |
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| Abstract: | Objective To investigate the changes of microRNA-15 a(miR-15 a), microRNA-16(miR-16), microRNA-20 b(miR-20 b) and their relationship with diabetic retinopathy(DR) in patients with type 2 diabetes mellitus(T2 DM). Methods A total of 105 patients with T2 DM admitted to Lishui People's Hospital from May 2017 to May 2019 were divided into non-DR group(32 cases), non-proliferative diabetic retinopathy(NPDR) group(39 cases) and proliferative diabetic retinopathy(PDR) group(34 cases), and 40 cases of simple cataract patients in the same period were selected as control group. The levels of miR-15 a, miR-16 and miR-20 b in aqueous humor were detected by direct determination of miR-derived fragment length polymorphism(miRFLP). The levels of miR-15 a, miR-16 and miR-20 b in serum were measured by real-time fluorescence quantitative PCR(qRT-PCR). The predictive value of miR-15 a, miR-16 and miR-20 b levels in aqueous humor and serum for PDR was analyzed by ROC curve. Results The levels of miR-15 a, miR-16 and miR-20 b in aqueous humor and serum were PDR group, NPDR group, non-DR group and control group respectively, with significant differences(all P<0.05). There was a positive correlation between aqueous humor and serum levels of miR-15 a, miR-16 and miR-20 b in DR patients(all P<0.05). The area under the curve of predicting PDR was 0.912 and the sensitivity was 91.20%, the area under the curve of predicting PDR was 0.931 and the specificity was 92.30%, the predictive value was better than that of single application. Conclusion The increased levels of miR-15 a, miR-16 and miR-20 b in aqueous humor and serum of patients with T2 DM are associated with DR. |
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