| 木犀草苷对小鼠非酒精性脂肪肝病的作用研究 |
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| 引用本文： | 王秀芳,晏春根.木犀草苷对小鼠非酒精性脂肪肝病的作用研究[J].中华全科医学,2019,17(1):21-24,51. |
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| 作者姓名： | 王秀芳 晏春根 |
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| 作者单位： | 绍兴文理学院附属医院消化内科, 浙江 绍兴 312000 |
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| 基金项目： | 浙江省自然科学基金项目(LY15H180008) |
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| 摘 要： | 目的探讨木犀草苷对小鼠非酒精性脂肪肝病(NAFLD)的保护作用。方法将52只昆明小鼠随机分为对照组(11只)和模型组(41只),对照组给予标准饲料,模型组给予高脂饲料。喂养8周,对照组和模型组各随机取一只,经病理确认建模成功后,将模型组随机分为4组,NAFLD模型组、木犀草苷低50 mg/(kg·d)]、中100 mg/(kg·d)]、高200 mg/(kg·d)]剂量组,每组10只。木犀草苷组给予相应木犀草苷量灌胃,对照组和NAFLD模型组给予等量生理盐水灌胃。4周后处死小鼠,测定血清甘油三酯(TG)、血清总胆固醇(TC)、天冬氨酸氨基酶(AST)及丙氨酸氨基转移酶(ALT)水平,血清及肝组织总超氧化物歧化酶(T-SOD)、谷胱甘肽(GSH)及丙二醛(MDA)水平,肿瘤坏死因子(TNF-α)及白细胞介素-6(IL-6)水平;并观察各组肝组织切片变化。结果与正常对照组比较,NAFLD模型组TG、TC、AST及ALT水平明显升高,血清及肝组织MDA、TNF-α及IL-6水平显著升高(均P <0. 05),T-SOD、GSH水平明显下降(均P <0. 05)。与NAFLD模型组比较,木犀草苷低、中、高剂量组TG、TC、AST及ALT水平明显下降(均P <0. 05),血清及肝组织MDA、TNF-α及IL-6水平显著下降(均P <0. 05),T-SOD、GSH水平明显升高(均P <0. 05)。木犀草苷组脂肪变细胞数量较NAFLD模型组显著改善。结论木犀草苷减少非酒精性脂肪肝脂质的沉积,减轻氧化应激及炎症反应程度,对非酒精性脂肪肝具有较好的保护作用。
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| 关 键 词： | 非酒精性脂肪肝 木犀草苷 血脂 炎症 |
| 收稿时间： | 2018-08-04 |
Effect of Luteolin on nonalcoholic fatty liver disease in mice |
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| Authors: | WANG Xiu-fang YAN Chun-gen |
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| Affiliation: | Department of Gastroenterology, the Affiliated Hospital of Shaoxing University of Arts and Sciences, Shaoxing, Zhejiang 312000, China |
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| Abstract: | Objective To investigate the protective effect of luteolin on nonalcoholic fatty liver disease (NAFLD) in mouse. Methods A total of 52 Kunming mice were randomly divided into control group (11 mice) and model group (41 mice). The control group was given standard diet and the model group was given high fat diet. After 8 weeks of feeding, 1 mouse in control group and 1 mouse in model group were randomly selected to confirm the model success or failure by pathological test. After model success, mice in model group were randomly divided into 4 groups, including NAFLD model group and low dose group50 mg/(kg·d)], middle dose group100 mg/(kg·d)]and high dose group200mg/(kg·d)], 10 mice in each group. The mice in Luteolin group were given corresponding dose of luteolin, and the control group and the NAFLD model group were given the same amount of normal saline. After 4 weeks, the mice were sacrificed and serum triglyceride (TG), serum total cholesterol (TC), aspartate transaminase (AST) and alanine aminotransferase (ALT) levels in serum were measured, and oxide dismutase (T-SOD), glutathione (GSH) and malondialdehyde (MDA) levels, tumor necrosis factor (TNF-α) and interleukin-6 (IL-6) levels in serum and liver tissue totals were tested. Then liver tissue sections were observed. Results Compared to control group, the TG, TC, AST and ALT levels of NAFLD model group were significantly increased, MDA, TNF-α and IL-6 levels of serum and liver tissues were significantly increased (all P < 0.05), and the T-SOD, GSH levels of serum and liver tissues were significantly decreased (all P < 0.05). Compared to NAFLD model group, the TG, TC, AST and ALT levels of low, middle and high dose luteolin groups were significantly decreased (all P < 0.05), and the MDA, TNF-α and IL-6 levels of serum and liver tissues were significantly decreased (all P < 0.05), and T-SOD and GSH levels of serum and liver tissues were significantly increased (all P < 0.05). The cells number of fatty degeneration in luteolin group was significantly improved as compared with NAFLD model group. Conclusion Luteolin can reduce the deposition of nonalcoholic fatty liver lipids, reduce the degree of oxidative stress and inflammatory reaction, and protect nonalcoholic fatty liver. |
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| Keywords: | Nonalcoholic fatty liver Luteolin Blood lipids Inflammation |
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