| 云南省傈僳族儿童乳牙龋的唾液微生物研究 |
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| 作者姓名： | 拜娴 崔瑶 周庆 李晓丽 李玥晓 刘娟 张石楠 |
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| 作者单位： | 1.昆明医科大学附属口腔医院儿童口腔科，云南 昆明 650106 |
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| 基金项目： | 云南省卫生内设研究机构项目[2016NS118] |
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| 摘 要： | 目的 研究云南省5岁傈僳族高龋和无龋儿童的唾液优势菌,并分析与当地汉族儿童的差异.方法 选取云南省怒江州傈僳族高龋(dmfs≥6)及无龋儿童(dmfs=0)各20名,同时选取同样的高龋和无龋汉族儿童作为对照,采集唾液样本,运用illumina Hiseq平台对16SrRNA V4区进行单端测序,分析微生物的群落结构和多...
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| 关 键 词： | 傈僳族 儿童 龋病 16SrRNA 唾液微生物 |
| 收稿时间： | 2022-01-15 |
Salivary Microbial Profiles of Dental Caries among Lisu Preschool Children in Yunnan Province |
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| Affiliation: | 1.Dept. of Pediatric Dentistry，Affiliated Stomatological Hospital of Kunming Medical University，Kunming Yunnan 6501062.Shu Hui Dental Clinic，Kunming Yunnan 6500323.The First Clinic of Affiliated Stomatological Hospital of Kunming Medical University4.Dept. of Preventive Dentistry，Kunming Yunnan 650106，China |
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| Abstract: | Objective To study the salivary predominant bacteria in 5-year-old Lisu children with and without dental caries in Yunnan Province and to analyze the differences with local Han children. Methods 20 Lisu children with high caries （dmfs≥6） and 20 children without caries （dmfs = 0）were selected from Nujiang Prefecture of Yunnan Province. Local Han children were also selected with the same inclusion criteria as comparison. Saliva samples were collected. The 16SrRNA V4 hyper variable region was sequenced using illumina Hisequencing platform. The community structure and diversity of microorganisms were analyzed using Mothur software. Results A total of 3965 oral microbial species annotations （Operational taxonomic unit , OTU） were obtained based on 97% similarity clustering in Lisu and Han Chinese children with and without dental caries, belonging to 16 phyla, 23 classes, 57 orders, 102 families and 202 genera. The differences in Alpha diversity and richness indices among the four groups were not statistically significant （P > 0.05）, and the differences in principal co-ordinates analysis （PCoA analysis） among the four groups were not statistically significant （P > 0.05）. The abundance of Streptococcus in the Lisu caries-active group is higher than that of the caries-free group, while the abundance of Haemophilus, Escherichia-Shigella, Fusobacterium, Capnocytophaga, Megasphaera is lower than that of the caries-free group. For caries-active children, the abundance of Gemella was higher in Lisu than in Han, and the abundance of Escherichia-Shigella was lower in Lisu than in Han. Conclusions The diversity, richness and composition of salivary microbial communities were similar among the Lisu and Han children with and without dental caries in Yunnan. However, the dental caries bio-markers of the two ethnic minority were different. The reasons underline the findings need further invesitigation. |
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