| 低密度脂蛋白诱导骨髓间充质干细胞向平滑肌样细胞分化的作用 |
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| 引用本文： | 陈雪,孟寒,许梅,王珊珊,黄冠,阮秋蓉.低密度脂蛋白诱导骨髓间充质干细胞向平滑肌样细胞分化的作用[J].中国动脉硬化杂志,2009,17(8). |
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| 作者姓名： | 陈雪 孟寒 许梅 王珊珊 黄冠 阮秋蓉 |
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| 作者单位： | 华中科技大学同济医学院附属同济医院病理研究所,湖北省武汉市,430030 |
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| 摘 要： | 目的 探讨低密度脂蛋白诱导骨髓间充质干细胞向平滑肌样细胞分化及myocardin在其分化过程中的作用.方法 不同浓度血清培养骨髓间充质干细胞条件下加以低密度脂蛋白刺激.细胞分为常规培养组,常规培养+低密度脂蛋白刺激组(25 mg/L), 20%胎牛血清培养组, 20%胎牛血清+低密度脂蛋白刺激组.免疫细胞化学检测myocardin、平滑肌肌动蛋白及平滑肌肌球蛋白重链的表达、RT-PCR法检测myocardin、平滑肌肌动蛋白及平滑肌钙结合相关蛋白mRNA的表达.结果 免疫细胞化学结果显示常规培养+低密度脂蛋白刺激组(25 mg/L),20%胎牛血清培养组, 20%胎牛血清+低密度脂蛋白刺激组分别与常规培养组比较myocardin、平滑肌肌动蛋白及平滑肌肌球蛋白重链的表达增强(P<0.05);20%胎牛血清+低密度脂蛋白刺激组与20%胎牛血清组比较上述三种蛋白表达增强(P<0.05);平滑肌肌动蛋白及平滑肌肌球蛋白重链蛋白的表达与myocardin表达呈正相关(r=0.9018,P<0.05;r=0.7969,P<0.05).RT-PCR结果显示常规培养+低密度脂蛋白刺激组(25 mg/L), 20%胎牛血清培养组, 20%胎牛血清+低密度脂蛋白刺激组分别与常规培养组比较myocardin、平滑肌肌动蛋白及平滑肌钙结合相关蛋白mRNA表达明显增强(P<0.05);20%胎牛血清培养组, 20%胎牛血清+低密度脂蛋白刺激组比较上述三个指标的表达也增强(P<0.05).平滑肌肌动蛋白、平滑肌钙结合相关蛋白mRNA表达与myocardin正相关(r=0.9295,P<0.05;r=0.8940,P<0.05).结论 高浓度血清,低密度脂蛋白均可诱导骨髓间充质干细胞向平滑肌样细胞分化,低密度脂蛋白联合20%胎牛血清诱导骨髓间充质干细胞向平滑肌样细胞分化的作用最强.myocardin可能在此过程中起重要作用.
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| 关 键 词： | 动脉粥样硬化 骨髓间充质干细胞 低密度脂蛋白 细胞分化 平滑肌样细胞 |
The Role of Low Density Lipoprotein in the Process of Bone Marrow Stromal Cells Differentiation into Smooth Muscle-like Cells |
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| CHEN Xue,MENG Han,XU Mei,WANG Shan-Shan,HUANG Guan,RUAN Qiu-Rong.The Role of Low Density Lipoprotein in the Process of Bone Marrow Stromal Cells Differentiation into Smooth Muscle-like Cells[J].Chinese Journal of Arteriosclerosis,2009,17(8). |
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| Authors: | CHEN Xue MENG Han XU Mei WANG Shan-Shan HUANG Guan RUAN Qiu-Rong |
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| Affiliation: | CHEN Xue,MENG Han,XU Mei,WANG Shan-Shan,HUANG Guan,and RUAN Qiu-Rong(Institute of Pathology,Tongji Hospital,Tongji Medical College,Hua zhong University of Science and Technology,Wuhan,430030) |
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| Abstract: | Aim To investigate the differentiation of marrow stromal cells induced by low density lipoprotein (LDL) in to smooth muscle-like cells and the role of myocardin in it.Methods Bone marrow stromal cells (BMSC) of mousewere purified by disparation adherence technique.The cultured BMSC were divided into 4 groups: regular cul-ture group, regular culture+LDL group (LDL 25 mg/L), 20% fetal bovine serum (FBS) culture group, 20% FBS cul-ture+LDL group.The expression of myocardin,α-SMA and SM-MHC were detected by immunocytochem istry technique.RT-PCR was used to detect the mRNA expression of myocardin,α-SMA and SM-22α.Results It is showed by immu-Nocytochem istry that the expression of myocardin,α-SMA and SM-MHC expressed in regular culture+LDL group(LDL 25 mg/L), 20% fetal bovine serum (FBS) culture group, 20% FBS culture+LDL groups were signifcnatly higher than that in regular culture group (P<0.05).The expression of the three proteins in 20% FBS culture+LDL group were signifi-cantly higher than that in 20% fetal bovine serum (FBS)group (P<0.05).The proteins expression ofα-SMA (r=0.9018,P<0.05) and SM-MHC(r=0.7969,P<0.05) were positively related with myocardin.It is approved by RT-PCR that the mRNA expression of myocardin,α-SMA and SM-22αin regular culture+LDL group(LDL 25 mg/L) 20%fetal bovine serum (FBS), 20% FBS culture+LDL group.groups were significantly higher than that in regular culture group (P<0.05).The mRNA expression of the three genes in 20% FBS culture+LDL group were significantly higher than that in 20% fetal bovine serum (FBS) group(P<0.05).The mRNA expression ofα-SMA (r=0.9295,P<0.05) and SM-22α(r=0.8940,P<0.05) were positively related with myocardin.Conclusion Both 20% FBS and LDL could induce the differentiation of BMSC into smooth muscle-like cells.The role of LDL with 20% FBS inducing the differentiation of BMSC into smooth muscle-like cells was the strongest.Andmyocardinmay play an important role in this differentiated process. |
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| Keywords: | myocardin |
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