| 慢性乙型肝炎患者肝组织LXRα和CYP7A1基因水平变化及其与肝组织炎症和纤维化程度的关系研究* |
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| 引用本文： | 李振,宁姗姗,郭影,王甜甜,李莉华,叶美红,宋源源.慢性乙型肝炎患者肝组织LXRα和CYP7A1基因水平变化及其与肝组织炎症和纤维化程度的关系研究*[J].实用肝脏病杂志,2022,25(2):191-194. |
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| 作者姓名： | 李振 宁姗姗 郭影 王甜甜 李莉华 叶美红 宋源源 |
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| 作者单位： | 472000 河南省三门峡市 河南科技大学附属黄河医院病理科(李振,郭影,王甜甜,叶美红,宋源源);检验科(宁姗姗,李莉华) |
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| 基金项目： | 河南省科技厅科研计划项目(编号:1923289)。 |
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| 摘 要： | 目的 探讨慢性乙型肝炎(CHB)患者肝X受体α(LXRα)和细胞色素P450亚型7A1(CYP7A1)基因表达水平与肝组织病理学炎症和纤维化程度的关系。方法 2019年1月～2020年10月我院收治的CHB患者118例,均接受肝穿刺活检,将炎症活动度分级＞G2和肝纤维化分期＞S2定义为肝组织炎症或纤维化程度显著;采用免疫组织化学染色法检测肝组织LXRα和CYP7A1表达,采用RT-PCR法检测LXRα mRNA和CYP7A1 mRNA水平。结果 118例CHB患者肝组织LXRα和CYP7A1蛋白和/或其mRNA阳性分别为78.0%和73.7%;38例肝组织显著炎症组LXRα mRNA和其蛋白(AOD)水平分别为(0.6±0.2)和(0.3±0.1),显著高于80例非显著炎症患者分别为(0.4±0.1)和(0.1±0.0),P<0.05],CYP7A1 mRNA和其蛋白(AOD)水平分别为(0.8±0.2)和(0.4±0.1),显著高于非显著炎症患者分别为(0.3±0.1)和(0.1±0.0),P<0.05];48例显著肝纤维化组肝组织LXRα mRNA和其蛋白(AOD)水平分别为(0.7±0.2)和(0.3±0.1),显著高于70例非显著肝纤维化患者分别为(0.3±0.1)和(0.1±0.1),P<0.05],CYP7A1 mRNA和其蛋白(AOD)水平分别为(0.7±0.2)和(0.3±0.1),显著高于非显著肝纤维化患者分别为(0.4±0.2)和(0.2±0.1),P<0.05]。结论 LXRα和CYP7A1表达上调可能参与了CHB患者肝组织炎症和肝纤维化发生发展过程,其机制值得进一步研究。
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| 关 键 词： | 慢性乙型肝炎 肝X受体α 细胞色素P450亚型7A1 肝组织学 |
| 收稿时间： | 2021-05-19 |
Hepatic expression of liver X receptorα and cytochrome P450 isoform 7A1 in patients with chronic hepatitis B |
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| Li Zhen,Ning Shanshan,Guo Ying,et al.Hepatic expression of liver X receptorα and cytochrome P450 isoform 7A1 in patients with chronic hepatitis B[J].Journal of Clinical Hepatology,2022,25(2):191-194. |
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| Authors: | Li Zhen Ning Shanshan Guo Ying |
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| Affiliation: | Department of Pathology, Huanghe Hospital Affiliated to Henan University of Science and Technology,Sanmenxia 472000, Henan Province,China |
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| Abstract: | Objective The aim of this study was to explore the relationship between liver X receptorα(LXRα)and cytochrome P450 isoform 7A1(CYP7A1)expression and liver histopathological inflammatory grading and fibrotic staging in patients with chronic hepatitis B(CHB).Methods 118 patients with CHB were enrolled in our hospital between January 2019 and October 2020,all patients underwent liver biopsies and histological activity>=G2 and liver fibrosis>=S2 were defined as significant inflammation or significant fibrosis.The expression and their mRNA levels of LXRαand CYP7A1 in liver tissues were detected by immunohistochemical staining and by RT-PCR.Results The positive rates of hepatic LXRα和CYP7A1mRNA and/or their proteins in 118 patients with CHB were 78.0%and 73.7%,respectively;the LXRαmRNA level and its protein expression in 38 patients with significant liver inflammation were(0.6±0.2)and(0.3±0.1)average optical density(AOD),significantly higher or stronger than(0.4±0.1)and(0.1±0.0)AOD,respectively,P<0.05]in 80 patients without significant hepatic histopathological inflammation,and the CYP7A1 mRNA level and its protein expression were(0.8±0.2)and(0.4±0.1)AOD,significantly higher or stronger than(0.3±0.1)and(0.1±0.0)AOD,respectively,P<0.05]in patients without significant hepatic inflammation;the LXRαmRNA and its protein expression in 48 patients with significant liver fibrosis were(0.7±0.2)and(0.3±0.1)AOD,significantly higher or stronger than(0.3±0.1)and(0.1±0.1)AOD,P<0.05]in 70 patients without significant liver fibrosis,and the CYP7A1 mRNA level and its protein expression were(0.7±0.2)and(0.3±0.1)AOD,significantly higher or stronger than(0.4±0.2)and(0.2±0.1)AOD,respectively,P<0.05]in patients without significant liver fibrosis.Conclusion The up-regulation of LXRαand CYP7A1 gene might be involved in the pathogenesis of liver inflammation and fibrosis,which needs further investigation. |
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| Keywords: | Hepatitis B Liver X receptorα Cytochrome P450 isoform 7A1 Liver histology |
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