| 孤儿核受体Nur77参与AopE-/-小鼠颈动脉易损斑块的形成 |
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| 引用本文： | 蔡招华,聂鹏,于瀛,崔明丽,孙士群,沈玲红,何奔.孤儿核受体Nur77参与AopE-/-小鼠颈动脉易损斑块的形成[J].心脏杂志,2014,26(4):373-377. |
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| 作者姓名： | 蔡招华 聂鹏 于瀛 崔明丽 孙士群 沈玲红 何奔 |
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| 作者单位： | (上海交通大学医学院附属仁济医院心内科，上海 200127) |
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| 基金项目： | 国家自然科学基金面上项目资助（30971185,81070239） |
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| 摘 要： | 目的:研究孤儿核受体Nur77在AopE-/-小鼠动脉粥样硬化易损斑块形成中的作用。方法:AopE-/-小鼠左肾动脉和左颈总动脉联合部分结扎建立颈动脉易损斑块模型,采用HE染色观察斑块病理学改变,免疫荧光染色结合激光扫描共聚焦显微镜技术观察斑块中Nur77蛋白的表达。同时,体外培养小鼠巨噬细胞系RAW264.7,在氧化低密度脂蛋白(oxLDL)及其主要成分7-酮胆固醇(7-ketocholesterol,7-KC)或者游离胆固醇(FC),以及炎症介质(LPS、IL-1β)等条件刺激下,应用蛋白免疫印迹技术(Western blot)检测Nur77蛋白表达变化。结果:AopE-/-小鼠颈动脉易损斑块局部有Nur77蛋白高表达,而oxLDL及其组成成分(7-KC和FC)以及某些炎症介质(如LPS、IL-1β等)均能在RAW264.7细胞中诱导Nur77蛋白表达上调。结论:孤儿核受体Nur77可能在动脉粥样硬化易损斑块发展进程中发挥着重要作用。
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| 关 键 词： | 动脉粥样硬化 易损斑块 巨噬细胞 核受体 Nur77 小鼠 |
| 收稿时间： | 2014-03-12 |
Orphan receptor Nur77 participates in formation of vulnerable carotid atherosclerotic plaque in ApoE-/- mice |
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| CAI Zhao-hua,NIE Peng,YU Ying,CUI Ming-li,SUN Shi-qun,Shen Ling-hong,HE Ben.Orphan receptor Nur77 participates in formation of vulnerable carotid atherosclerotic plaque in ApoE-/- mice[J].Chinese Heart Journal,2014,26(4):373-377. |
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| Authors: | CAI Zhao-hua NIE Peng YU Ying CUI Ming-li SUN Shi-qun Shen Ling-hong HE Ben |
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| Affiliation: | (Department of Cardiology, Renji Hospital, Medical College, Shanghai Jiaotong University, Shanghai 200127, China) |
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| Abstract: | AIM ： To investigate whether orphan receptor Nur77 is involved in the formation of vulnerable carotid atherosclerotic plaque in ApoE-/- mice. METHODS ： Using the method of partial ligation of left carotid and left renal arteries, we generated an ApoE-/- mouse model of vulnerable carotid atherosclerotic plaque. The pathological changes of atherosclerotic plaques were observed using H/E staining and the expression of Nur77 was detected by laser confocal microscopy after immunofluorescence staining. Macro- phages （ murine RAW 264.7 cells） were cultured and were stimulated with oxLDL and its main lipid components 7-ketocholesterol （7-KC） or free cholesterol （ FC ） and some inflammatory mediators （ LPS or IL-113）. The protein expression of Nur77 was detected by Western blotting. RESULTS ： Nur77 is highly expressed in vulnerable carotid atherosclerotic plaques in ApoE-/- mice. In addition, oxLDL and its main lipid components （7-KC or FC） and some inflammatory mediators （LPS or IL-113） induced the expres- sion of Nur77 in murine RAW 264.7 cells in vitro. CONCLUSION ： Orphan receptor Nur77 participates in the formation of vulnerable carotid atherosclerotic plaque in ApoE-/- mice. |
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| Keywords: | atherosclerosis vulnerable plaque macrophage nuclear receptor Nur77 mouse |
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