| Th1、Th17细胞相关因子在实验性自身免疫性葡萄膜炎大鼠中的表达及作用 |
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| 引用本文： | 钱丽丽,徐亚茹,胡芳,官杰,吴艳敏,王琪.Th1、Th17细胞相关因子在实验性自身免疫性葡萄膜炎大鼠中的表达及作用[J].眼科新进展,2015,0(10):918-920. |
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| 作者姓名： | 钱丽丽 徐亚茹 胡芳 官杰 吴艳敏 王琪 |
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| 作者单位： | 161006 黑龙江省齐齐哈尔市，齐齐哈尔医学院免疫教研室 |
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| 摘 要： | 目的 探讨辅助性Ｔ细胞（Ｔｈｅｌｐｅｒｃｅｌｌｓ，Ｔｈ）１、Ｔｈ１７细胞相关因子在实验性自身免疫性葡萄膜炎（ｅｘｐｅｒｉｍｅｎｔａｌａｕｔｏｉｍｍｕｎｅｕｖｅｉｔｉｓ，ＥＡＵ）中的表达及作用。方法 取清洁级纯系健康雌性Ｌｅｗｉｓ大鼠４０只随机分为ＥＡＵ组（３２只）和对照组（８只），ＥＡＵ组用光感受器间维生素Ａ类结合蛋白诱导大鼠ＥＡＵ模型，进行临床症状评分，免疫组织化学方法检测造模后视网膜内干扰素（ｉｎｆｅｒｆｅｒｏｎ，ＩＦＮ）-γ、诱导型一氧化氮合酶（ｉｎｄｕｃｉｂｌｅｎｉｔｒｉｃｏｘｉｄｅｓｙｎｔｈａｓｅ，ｉＮＯＳ）、白细胞介素（ｉｎｔｅｒｌｅｕｋｉｎｅ，ＩＬ）-１７、ＩＬ-６的表达。ＥＬＩＳＡ法对比分析房水中各细胞因子的变化情况。结果 ＥＡＵ模型建立成功；造模后第１４天，视网膜损害以外层为主，视网膜内有大量炎细胞浸润，从而导致视网膜内结构紊乱，同时在视网膜的视锥、视杆细胞层和神经节细胞层ｉＮＯＳ、ＩＬ-１７、ＩＬ-６、ＩＦＮ-γ表达，细胞阳性率分别为２９％、４８％、５２％、７３％。在ＥＡＵ发病过程中，ＩＬ-６于造模后第７天迅速升高，第１０天达到高峰；ＩＬ-１７于造模后第１４天达到最高值，变化趋势与炎症进程一致；ＩＦＮ-γ在炎症后期仍有升高，于造模后第１６天达到最高值；ＩＬ-６、ＩＬ-１７、ＩＦＮ-γ与对照组相比，各时间点表达差异均有统计学意义（均为Ｐ＜０．０５）。ｉＮＯＳ在炎症进程中表达有所增加，但与对照组相比，各时间点的表达差异均无统计学意义（均为Ｐ＞０．０５）。结论 Ｔｈ１、Ｔｈ１７细胞相关因子调节网络共同参与实验性自身免疫性葡萄膜炎的发生发展。
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| 关 键 词： | 辅助性T细胞1 辅助性T细胞17 实验性自身免疫性葡萄膜炎 大鼠 |
Expression and roles of Th1,Th17 cytokines in experimental autoimmune uveitis of rats |
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| QIAN Li-Li,XU Ya-Ru,HU Fang,GUAN Jie,WU Yan-Min,WANG Qi.Expression and roles of Th1,Th17 cytokines in experimental autoimmune uveitis of rats[J].Recent Advances in Ophthalmology,2015,0(10):918-920. |
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| Authors: | QIAN Li-Li XU Ya-Ru HU Fang GUAN Jie WU Yan-Min WANG Qi |
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| Affiliation: | Department of Immunology , Qiqihar Medical College, Qiqihar 161006 , Heilongjiang Province . China |
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| Abstract: | Objective To investigate the expression and roles of Thl , Th17 cytokines in experimental autoimmune uveitis ( EAU ) of rats. Methods Forty clean healthy female Lewis rats were randomly divided into EAU group ( 32 cases) and control group ( 8 cases) . EAU was induced by immunization with interphotoreceptor retinoid-binding protein( IRBP) in EAU group , the clinical scoring were applied, immunohistochemistry was employed to detect the expression of IFN-7 ,iNOS , IL-6 . IL-17 in retina after establishment of models. The cytokines levels changes m aqueous humor were observed and compared by ELISA. Results Rat EAU models were constructed successfully. At 14 days after establishment of models .the retinal damage mainly located in the outer layer, and there was a large number of inflammatory cells infiltrating the retina, which leaded to the retinal structural disorder. iNOS , IL-17 .IL-6 and IFN-7 expressed in the cone . rod . and ganglion cell layers of the retina , the positive cell rates were 2g% ,48% ,52% ,73% . respectively. During the course of EAU ,IL-6 increased rapidly at 7 days after modeling , and reached the peak at 10 days ;IL-17 reached the highest level at 14 days after modeling , and the change trend was consistent with inflammation process. IFN-7 was still elevated in the late stage of inflammation and reached the highest level at 16 days after modeling;Compared with the control group, the expression of IL-6, IL-17 and IFN-7 were significantly different at each time point( all P < 0. 05 ) . The expression of iNOS increased in the inflammatory process , but the expression at each time point was not significantly different from the control group ( all P > 0. 05 ) . Conclusion The cytokine regulating network of Thl and Th17 participate in the development of EAU. |
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| Keywords: | Th1 Th17 experimental autoimmune uveitis rats |
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