| 雷公藤红素抑制MGC - 823细胞增殖和迁移及诱导凋亡 |
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| 引用本文： | 王红,李诗楠,李智,赵楠,张欣,杜天宇.雷公藤红素抑制MGC - 823细胞增殖和迁移及诱导凋亡[J].现代预防医学,2020,0(14):2602-2606. |
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| 作者姓名： | 王红 李诗楠 李智 赵楠 张欣 杜天宇 |
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| 作者单位： | 1.锦州医科大学附属第一医院转化医学研究院，辽宁 锦州 121000；2.锦州医科大学转化医学研究院，辽宁 锦州 121000；3.锦州医科大学附属第一医院心胸外科，辽宁 锦州 121000；4.锦州医科大学附属第一医院胸外科，辽宁 锦州 121000 |
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| 摘 要： | 目的 探索雷公藤红素对MGC - 823细胞的增殖，迁移及诱导凋亡的作用，并探讨其作用机制。方法 0、1、2、4、6、8、12 μM雷公藤红素作用于MGC - 823细胞，采用MTT法检测增殖比率。0、1、2和4 μM雷公藤红素作用于MGC - 823细胞0、24、48和72 h，显微镜下观察细胞形态，采用细胞划痕实验检测细胞迁移能力，流式细胞术检测细胞凋亡，western blot法检测细胞中凋亡相关蛋白Bcl - 2、Bax、Akt、p - Akt等的表达水平。结果 经雷公藤红素作用后，MGC - 823细胞的增殖率均下降（P＜0.05）；细胞数量减少，体积缩小，皱缩变圆，部分不再贴壁，细胞核缩小；细胞迁移率减小（P＜0.05）；早期凋亡率增加（P＜0.05）；MGC - 823细胞中Bax表达升高，Bcl - 2和p - Akt表达降低（P＜0.05）。结论 雷公藤红素对MGC - 823细胞的增殖和迁移有抑制作用，并诱导其凋亡。
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| 关 键 词： | 胃癌 MGC - 823细胞 增殖 迁移 凋亡 |
Effects of celastrol on proliferation,migration and induction of apoptosis in MGC-823 cells |
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| WANG Hong,LI Shi-nan,LI Zhi,ZHAO Nan,ZHANG Xin,DU Tian-yu.Effects of celastrol on proliferation,migration and induction of apoptosis in MGC-823 cells[J].Modern Preventive Medicine,2020,0(14):2602-2606. |
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| Authors: | WANG Hong LI Shi-nan LI Zhi ZHAO Nan ZHANG Xin DU Tian-yu |
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| Affiliation: | *Institute of Translational Medicine, First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning 121000, China |
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| Abstract: | To investigate the effect of celastrol on the proliferation, migration and apoptosis of MGC-823 cells, and to explore its mechanism. Methods 0, 1, 2, 4, 6, 8, 12 μM celastrol was applied to MGC-823 cells, and the proliferation ratio was detected by MTT assay. 0, 1, 2, and 4 μM celastrol were applied to MGC-823 cells at 0 h, 24 h, 48 h, and 72 h,cell morphology was observed under a microscope, and cell migration ability was measured by cell scratch assay. Theapoptosis was detected by cytometry, and the expression levels of apoptosis-related proteins Bcl-2, Bax, Akt and p-Akt were detected by Western blot. Results After the action of celastrol, the proliferation rate of MGC-823 cells decreased (P<0.05);the number of cells decreased, the volume decreased, the shrinkage became round, some of them no longer adhered, the nuclei narrowed, and the cell migration rate decreased (P<0.05); early apoptotic rate increased (P<0.05); Bax expression increased and Bcl -2 and p -Akt expression decreased in MGC -823 cells (P <0.05). Conclusion Celastrol inhibited the proliferation and migration of MGC-823 cells and induced apoptosis. |
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| Keywords: | Celastrol MGC-823 cell Proliferation Migration Apoptosis |
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