| RIPK3在皮肤基底细胞癌中的表达及意义 |
| |
| 引用本文： | 张梦迪,陈彩凤,段琪琪,王 宁,王 敏,刘 萌,邵永平,郑 焱.RIPK3在皮肤基底细胞癌中的表达及意义[J].现代肿瘤医学,2019,0(17):3105-3109. |
| |
| 作者姓名： | 张梦迪 陈彩凤 段琪琪 王 宁 王 敏 刘 萌 邵永平 郑 焱 |
| |
| 作者单位： | 1.西安交通大学第二附属医院皮肤科，陕西 西安 710004；2.福建省立医院，福建 福州 350001；3.西安交通大学前沿科学技术研究院，生命科学与技术学院，教育部生物医学信息工程重点实验室，陕西 西安 710049 |
| |
| 基金项目： | 陕西省中青年科技创新领军人才项目（编号:SQ2019RA2G000107） |
| |
| 摘 要： | 目的:研究RIPK3在皮肤基底细胞癌中的表达及意义。方法:应用免疫组化SP法检测皮肤基底细胞癌和正常皮肤组织中RIPK3蛋白的表达情况。用RIPK3特异性siRNA干扰RIPK3在原代表皮角质形成细胞HEKa中的表达，用Annexin V-FITC/PI双染法检测细胞凋亡的变化。结果:在所收集的正常皮肤组织中，RIPK3的阳性表达率为90.0%，而在皮肤基底细胞癌皮损中其表达呈明显下降，阳性率为7.5%，两组RIPK3蛋白的阳性表达率及表达强度的差异均具有统计学意义（P<0.001）。HEKa细胞特异性敲低RIPK3表达后细胞凋亡数量减少。结论:RIPK3在皮肤基底细胞癌中的表达显著低于正常皮肤组织，RIPK3在皮肤基底细胞癌中的异常表达可能是通过抑制细胞程序性死亡而在其发生发展过程中起作用。
|
| 关 键 词： | 皮肤基底细胞癌 RIPK3 免疫组化 细胞凋亡 |
The expression and significance of RIPK3 in skin basal cell carcinoma |
| |
| Zhang Mengdi,Chen Caifeng,Duan Qiqi,Wang Ning,Wang Min,Liu Meng,Shao Yongping,Zheng Yan.The expression and significance of RIPK3 in skin basal cell carcinoma[J].Journal of Modern Oncology,2019,0(17):3105-3109. |
| |
| Authors: | Zhang Mengdi Chen Caifeng Duan Qiqi Wang Ning Wang Min Liu Meng Shao Yongping Zheng Yan |
| |
| Affiliation: | 1.Department of Dermatology，the Second Affiliated Hospital of Xi＇an Jiaotong University，Shaanxi Xi＇an 710004，China；2.Fujian Provincial Hospital，Fujian Fuzhou 350001，China；3.Key Laboratory of Biomedical Information Engineering of the Ministry of Education，School of Life Science and Technology，Frontier Institute of Science and Technology，Xi＇an Jiaotong University，Shaanxi Xi＇an 710049，China. |
| |
| Abstract: | Objective:To investigate the expression and significance of RIPK3 protein in the skin basal cell carcinoma（BCC）.Methods:The SP method of immunohistochemistry was carried out to detect the expression of RIPK3 protein in BCC and normal skin tissue.RIPK3-specific siRNA was used to interfere with the expression of RIPK3 in the primary keratinocytes HEKa，and cell apoptosis was detected by Annexin V-FITC/PI staining flow cytometry.Results:In the normal skin，the positive expression rate of RIPK3 protein was 90.0%，while the expression of RIPK3 protein was significantly decreased in the skin basal cell carcinoma and the positive rate was 7.5%.The difference in positive expression rate and expression intensity of RIPK3 protein between the two groups was statistically significant (P<0.001).The number of apoptosis cells was decreased when expression of RIPK3 was specifically knocked down in HEKa cells.Conclusion:The expression of RIPK3 protein in skin basal cell carcinoma is significantly lower than that in normal skin tissue.The abnormal expression of RIPK3 in skin basal cell carcinoma may play a role in the development and progression by inhibiting programmed cell death. |
| |
| Keywords: | skin basal cell carcinoma RIPK3 immunohistochemistry cell apoptosis |
|
| 点击此处可从《现代肿瘤医学》浏览原始摘要信息 |
|
点击此处可从《现代肿瘤医学》下载全文 |
