| 口腔黏膜癌变过程中中心体的扩增及其意义 |
| |
| 引用本文： | 蔡扬,李秉琦,陈谦明.口腔黏膜癌变过程中中心体的扩增及其意义[J].华西口腔医学杂志,2004,22(3):238-241. |
| |
| 作者姓名： | 蔡扬 李秉琦 陈谦明 |
| |
| 作者单位： | 1.口腔生物医学工程教育部重点实验室,四川大学,四川 成都610041; 2.四川大学华西口腔医院 口腔黏膜病科,四川 成都610041 |
| |
| 基金项目： | 国家自然科学基金资助项目 (30 0 70 81 5)，国家留学基金委资助项目 (2 0 852 0 60 ) |
| |
| 摘 要： | 目的 了解口腔黏膜癌变过程中中心体的状况,探讨中心体的异常在口腔鳞状细胞癌(OSCC)发生发展中的作用及其在口腔黏膜癌变过程中的意义。方法 选取正常口腔黏膜(12例)、轻度上皮异常增生(2例)、中度上皮异常增生(8例)、重度上皮异常增生(12例),口腔高分化鳞癌(10例)、中分化鳞癌(15例)、低分化鳞癌(7例)的石蜡包埋组织,采用间接免疫荧光双重染色(γ-微管蛋白单克隆抗体及细胞角蛋白多克隆抗体)显示上皮细胞中的中心体,分析其在口腔黏膜癌变过程中的变化趋势及在各组间的差异。结果 正常口腔黏膜上皮表现为大小数目正常的中心体,但在72·73%(16/22)的上皮异常增生及84·38%(27/32)OSCC组织中观察到部分上皮或肿瘤细胞中出现异常中心体,表现为中心体直径的增加或数目的增多。具有异常中心体的细胞数目随上皮异常增生程度的增加及肿瘤分化程度的降低而增加,二者存在明显的正相关关系(P<0·01)。结论 中心体的扩增是口腔黏膜癌变过程中的早期事件并与口腔癌发生发展进程有关,口腔黏膜癌变过程中的细胞形态学改变与中心体扩增之间可能存在直接机制上的联系。从调控中心体循环入手治疗口腔癌前病损及OSCC,有可能成为口腔癌预防和治疗的新途径。
|
| 关 键 词： | 口腔鳞状细胞癌 癌前病变 中心体 |
| 文章编号： | 1000-1182(2004)03-0238-04 |
| 收稿时间： | 2004-06-25 |
| 修稿时间： | 2003年11月5日 |
Centrosome Hyperamplification in Oral Precancerous Lesions and Squamous Cell Carcinomas |
| |
| CAI Yang,LI Bing-qi,CHENG Qian-ming..Centrosome Hyperamplification in Oral Precancerous Lesions and Squamous Cell Carcinomas[J].West China Journal of Stomatology,2004,22(3):238-241. |
| |
| Authors: | CAI Yang LI Bing-qi CHENG Qian-ming |
| |
| Affiliation: | 1.Key. Laboratory ofOral Biomedical Engineering, Ministry ofEducation, Sichuan University, Chengdu 610041, China;2.Dept. ofOral Medicine,West China College ofStomatology, Sichuan University, Chengdu610041, China |
| |
| Abstract: | Objective It is currently considered that the defect of mitotic spindle caused by centrosome abnormalities may be one of the reasons for the development of aneuploidy in tumors. This study attempted to elucidate the possible role of centrosome defects in the development and progression of OSCC by investigating the frequency of centrosome amplification in oral precancerous lesions and OSCC.Methods Formalin-fixed, paraffin-embedded tissues of 12 cases of normal oral epithelium, 22 case of dysplasia with different degree epithelium dysplasia and 32 cases of OSCC with different differentiation were investigated for centrosome status by using indirect Immunofluorescence double staining with antibodies to centrosome protein gamma-tubulin and cytokeratin. The differences and the change trend of centrosome status in these groups were statistical analyzed by SPSS10.0.Results Normal oral epithelium showed normal centrosomes in epithelium cells, while 16 of 22 cases (72.73%) of dysplasia(DYS) and 27 of 32 cases (84.38%) of OSCC showed the evidence of centrosome amplification and morphological abnormalities characterized by huge size, clump or supernumerary centrosomes in a fraction of epithelium or tumor cells. The percentage of cells with abnormal centrosomes increased gradually from mild-dysplasia epithelium to poorly differentiated OSCC, which positively correlated with the histologicalcytologic grade of oral precancerous lesions and OSCC(P<0.01).Conclusion Centrosome amplification was an early event and that might play a role in the establishment and perhaps the progression of OSCC. There might be some direct relationship between centrosome defects and the cellular morphological phenotype characteristics of dysplasia and OSCC. Centrosome amplification could be served as an alternative diagnostic indicator of dysplasia and the intervention of centrosome cycle might serve as a particular way for the prevention and treatment of OSCC in the future. |
| |
| Keywords: | oral squamous cell carcinomas (OSCC) precancerous lesion centrosome |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《华西口腔医学杂志》浏览原始摘要信息 |
|
点击此处可从《华西口腔医学杂志》下载全文 |
|
