Complete Genome Sequence and Genome Analysis of Eggplant mottled dwarf virus‐Iranian Isolate |
| |
| Authors: | Ghobad Babaie Mina Kouhi Habibi Amir Massah Akbar Dizadji Laleh Izadinejad Anne Simon |
| |
| Affiliation: | 1. Department of Plant Protection, Faculty of Agricultural Sciences & Engineering, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran;2. Agriculture and Natural Resources Research Center of Shahrekord, Shahrekord, Iran;3. Department of Plant Protection, College of Agriculture, Isfahan University of Technology, Isfahan, Iran;4. Plant Protection Organization, Tehran, Iran;5. Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD, USA |
| |
| Abstract: | The full‐length nucleotide sequence of the Iranian isolate of Eggplant mottled dwarf virus (EMDV), a phytorhabdovirus, was determined using the random polymerase chain reaction method (rPCR) followed by PCR with specific primers to fill in the gaps. The negative‐sense RNA genome of the Iranian isolate of EMDV contains 13154 nucleotides and seven open‐reading frames (ORFs) in the order 3′‐leader‐N‐X‐P‐Y‐M‐G‐L‐trailer‐5′. These ORFs encode the nucleocapsid, X protein (of unknown function), phosphoprotein, Y protein (putative movement protein), matrix protein, glycoprotein and RNA‐dependent RNA polymerase, respectively. EMDV has a 199 nt 3′ leader RNA and a 151 nt 5′ trailer, and the ORFs are separated by conserved intergenic sequences. Phylogenetic analyses indicate that EMDV is most closely related to Potato yellow dwarf virus, which has a distinctly different geographical distribution. |
| |
| Keywords: |
Eggplant mottled dwarf virus
Iran nucleorhabdoviruses
Rhabdoviridae
|
|
|
