| 金属离子抗性衣藻品系的分离筛选及其鉴定 |
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| 引用本文： | 董志芳,艾山江·阿布都拉,阿布力孜·阿布力米提,图尔逊·买买提,吾甫尔·米吉提.金属离子抗性衣藻品系的分离筛选及其鉴定[J].武汉植物学研究,2010,28(1):21-26. |
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| 作者姓名： | 董志芳 艾山江·阿布都拉 阿布力孜·阿布力米提 图尔逊·买买提 吾甫尔·米吉提 |
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| 作者单位： | 新疆大学生命科学与技术学院,乌鲁木齐,830017 |
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| 基金项目： | 新疆生物资源基因工程重点实验室开放基金 |
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| 摘 要： | 从乌鲁木齐南山土壤中分离得到62株绿藻,利用印迹法筛选对Cu2＋、Fe3＋、Zn2＋、Co2＋4种金属离子有抗性的藻株。结果发现XJU-3、XJU-28和XJU-36对0.1mmol·L-1 Co2＋有抗性;XJU-28对1mmol·L-1 Zn2＋和Fe3＋有抗性;而XJU-36仅对0.05mmol·L-1 Cu2＋有抗性。利用形态学特征和rDNA转录单元内间隔区（ITS1和TIS2,包括5.8S）序列对3株绿藻进行了分类学鉴定。依据形态特征,初步判断3株绿藻可能属于衣藻属（Chlamydomonas）。利用ITS（包括5.8S）序列构建系统进化树分析,结果表明,XJU-3、XJU-28与Chlamydomonas zebra的关系较近,XJU-36与Chlamydomonas petasua的关系较近。
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| 关 键 词： | 衣藻 金属离子抗性 形态特征 转录间隔区（ITS） |
Isolation and Identification of Metal Resistance Green Alga |
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| DONG Zhi-Fang,Hasanjan Abdulla,Abliz Ablimit,Tursun Mamat,Gopur Mijit.Isolation and Identification of Metal Resistance Green Alga[J].Journal of Wuhan Botanical Research,2010,28(1):21-26. |
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| Authors: | DONG Zhi-Fang Hasanjan Abdulla Abliz Ablimit Tursun Mamat Gopur Mijit |
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| Affiliation: | DONG Zhi-Fang,Hasanjan Abdulla,Abliz Ablimit,Tursun Mamat,Gopur Mijit(College of Life Science , Technology,Xinjiang University,Urumqi 830046,China) |
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| Abstract: | A total of 62 green alga strains were isolated from the soils of Nanshan Mountain, Xinjiang. Used the blot method to characterize these metal resistance, the results indicated that XJU-3、XJU-28 and XJU-36 have resistance to 0. 1 mmol · L~(-1) Co~(2+) ;XJU-28 has resistance to 1 mmol·L~(-1) Zn~(2+) and Fe~(3+) , XJU-36 has resistance to 0. 05 mmol·L~(-1) Cu~(2+). Taxonomic evalua-tion of the three strains were investigated based on the morphology and the internal transcribed spacer (ITS) regions (including the 5. 8S). Based on morphological characteristics, the three strains were likely to Chlamydomonas. Phylogenetic reconstruction with the Neighbor-joining (NJ) method using sequences of ITS(including the 5. 8S) indicated that XJU-3 and XJU-28 are closed to Chlamydomonas zebra. XJU-36 is closed to Chlamydornonas petasua. |
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| Keywords: | Chlamydomonas Metal resistance Morphologic ITS |
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