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| 拟南芥与大豆疫霉菌的非寄主互作及一个感病突变体的遗传分析 | |
| 引用本文： | 刘秋萍,曹华,姚茂金,马英,邓斌生,权军利,单卫星.拟南芥与大豆疫霉菌的非寄主互作及一个感病突变体的遗传分析[J].植物学报,2010,45(5):548-555. |
| 作者姓名： | 刘秋萍 曹华 姚茂金 马英 邓斌生 权军利 单卫星 |
| 作者单位： | 西北农林科技大学植物保护学院, 杨凌 712100; 西北农林科技大学生命科学学院, 杨凌 712100 西北农林科技大学农学院, 杨凌 712100; 西北农林科技大学陕西省农业分子生物学重点实验室, 杨凌 712100 |
| 基金项目： | 国家大学生创新性实验计划,教育部新世纪优秀人才支持计划,代农业产业技术体系 |
| 摘 要： | 非寄主抗病性是一种普遍的自然现象,该文通过建立拟南芥-大豆疫霉菌(Arabidopsis thaliana-Phytophthora sojae)非寄主互作系统,筛选对大豆疫霉菌感病的拟南芥突变体,为研究植物对卵菌的非寄主抗病性遗传机制奠定基础。以大豆疫霉菌游动孢子接种拟南芥T-DNA插入突变体离体叶片,从代表12000个独立转化株系的40000株T3代T-DNA插入拟南芥突变体中获得一系列对大豆疫霉菌感病的突变体。其中突变体581-51感病性状表现稳定,离体叶片接菌后3天内出现明显的水渍状病斑,4-5天后产生大量卵孢子和/或孢子囊。细胞学观察发现有典型的吸器形成。Southern杂交和遗传分析结果表明,581-51突变体含有4个T-DNA插入事件,其感病性状可能由隐性单基因控制。 |
| 关 键 词： | 拟南芥 非寄主抗病性 卵菌 大豆疫霉菌 T-DNA插入突变体 |
| 收稿时间： | 2009-09-23 |
| 修稿时间： | 2009-12-23 |
Nonhost Interaction of Phytophthora sojae and Arabidopsis thaliana and Genetic Analysis of a Susceptible Mutant |
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| Qiuping Liu,Hua Cao,Maojin Yao,Ying Ma,Binsheng Deng,Junli Quan,Weixing Shan.Nonhost Interaction of Phytophthora sojae and Arabidopsis thaliana and Genetic Analysis of a Susceptible Mutant[J].Bulletin of Botany,2010,45(5):548-555. | |
| Authors: | Qiuping Liu Hua Cao Maojin Yao Ying Ma Binsheng Deng Junli Quan Weixing Shan |
| Affiliation: | College of Plant Protection, Northwest A & F University, Yangling 712100, China; College of Life Sciences, Northwest A & F University, Yangling 712100, China; College of Agronomy, Northwest A & F University, Yangling 712100, China; Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A & F University, Yangling 712100, China |
| Abstract: | Phytophthora sojae and Arabidopsis thaliana were used in this study as a nonhost plant-oomycete interaction system to investigate the genetic basis of nonhost resistance against oomycete pathogens in plants. A collection of more than 40 000 T3 A. thaliana T-DNA mutant plants representing 12 000 independent insertion lines were screened by inoculating detached leaves with P. sojae zoospores, and the susceptible mutant was re-confirmed by pathogen inoculation and cytological characterization. A number of P. sojae-susceptible A. thaliana mutants were successfully obtained, and one of them, mutant 581-51, was shown to be stably susceptible to P. sojae infection. Water-soaked lesions formed on the detached leaves within 3 days, as did oospores and sporangia 4–5 days after inoculation with P. sojae zoospores. Cytological characterization revealed the formation of haustoria-like structures. Southern analysis showed the presence of four T-DNA insertion events in the mutant. Genetic analysis indicated that the susceptibility to infection by the nonhost pathogen P. sojae in the mutant 581-51 was likely controlled by a single recessive gene. |
| Keywords: | Arabidopsis thaliana" target="_blank">Arabidopsis thaliana')" href="#">Arabidopsis thaliana nonhost disease resistance oomycetes Phytophthora sojae" target="_blank">Phytophthora sojae')" href="#"> Phytophthora sojae T-DNA insertional mutants |
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