| 中国主要地方品种猪血液蛋白遗传多样性研究 |
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| 引用本文： | 吴小秋,张亚平,丁波,陈永久,熊绿芸,何照范.中国主要地方品种猪血液蛋白遗传多样性研究[J].动物学研究,1999,20(4):252-257. |
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| 作者姓名： | 吴小秋 张亚平 丁波 陈永久 熊绿芸 何照范 |
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| 作者单位： | 1. 中国科学院昆明动物研究所细胞与分子进化开放研究实验室,昆明,650223
2. 贵州农学院生化营养研究所,贵阳,550025 |
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| 基金项目： | 国家自然科学基金，云南省自然科学基金 |
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| 摘 要： | 实验采用水平板淀粉凝胶电泳技术，对中国地方猪五大类型９个品种，１个引入品种共２０１个个体进行了遗传多样性分析。共分析遗传座痊３０个，其中只有ＭＤＨ、ＰＥＰＢ、Ａ从位具有多在记民生。中国主要地方猪品种的多态座位百分比Ｐ＝０．２００，平均杂２合度Ｈ＝０．０６５，平均等位基因数Ａ＝１．３００。根据基因频率采用ＰＨＹＬＩＰ３．５ｃ民计算Ｎｅｉ氏遗传距离，然后用“ＮＥＩＧＨＢＯＲ”程序分别构建Ｎｅｉｇｈｂｏ
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| 关 键 词： | 家猪 蛋白多态 遗传多样性 |
GENETIC DIVERSITY OF BLOOD PROTEIN AMONG MAIN INDIGENOUS PIG BREEDS IN CHINA |
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| WU Xiao-qiu,ZHANG Ya-ping,DING Bo,CHEN Yong-jiu,XIONG Lu-yun,HE Zhao-fan.GENETIC DIVERSITY OF BLOOD PROTEIN AMONG MAIN INDIGENOUS PIG BREEDS IN CHINA[J].Zoological Research,1999,20(4):252-257. |
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| Authors: | WU Xiao-qiu ZHANG Ya-ping DING Bo CHEN Yong-jiu XIONG Lu-yun HE Zhao-fan |
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| Abstract: | A total of 201 blood samples including 9 indigenous pig breeds ( Sus scrofa domesticus ) in China and Yorkshine pigs were collected in order to evaluate the genetic diversity among Chinese pig breeds.Thirty blood protein loci were studied by horizontal starch gel electrophoresis,among which 6 loci (MDH,PEPB,AK,6PGD,ADA,PHI) were found to be polymorphic.The percentage of polymorphic loci ( P ),the mean individual heterozygosity ( H ) and the mean number of alleles ( A ) are 0 200,0 065 and 1 300,respectively.The degree of genetic variability of Chinese indigenous pig breeds as a whole was higher than that of goats,lower than those of cattle,horses and sheep.Using gene frequencies of the 30 loci,the pairwise genetic distance among the 9 indigenous breeds in China were calculated by software PHYLIP3 5?c.The phylogenetic trees were constructed using NJ and UPGMA methods.The results suggest that the geographical distribution do not consist with the genetic relationship among Chinese indigenous pig breeds.The genetic diversity among Chinese indigenous pig breeds is low. |
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| Keywords: | Domestic pig Chinese indigenous breeds Protein polymorphism Genetic diversity |
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