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Loop‐Mediated Isothermal Amplification (LAMP): A Rapid and Sensitive Tool for Quality Assessment of Meat Products
Authors:Yogesh Kumar  Sangita Bansal  Pranita Jaiswal
Affiliation:ICAR‐Central Inst. of Post‐Harvest Engineering and Technology (CIPHET), Ludhiana, India
Abstract:Loop‐mediated isothermal amplification (LAMP) is a novel method that amplifies target nucleic acids under isothermal conditions. It is a rapid, specific, and sensitive method, which does not require costly thermal cyclers for the detection of nucleic acids. Thus, it is suitable for on‐site detection assays under low‐resource settings. It can also be integrated on compact lab‐on‐a‐chip devices for the development of micro‐total analysis systems. This review discusses LAMP‐based methods, as well as LAMP‐based centrifugal, microfluidic, and other fluid‐handling devices, which have been developed for the assessment of meat quality parameters that are related to the presence or absence of nucleic acids, for example, animal species identification and microbiological quality. Advances in improving the rapidity, specificity, and sensitivity of LAMP techniques for the assessment of these meat quality parameters are also discussed in this review.
Keywords:LAMP  meat quality  lab‐on‐a‐chip  microfluidic  noninstrument nucleic acid amplification  micro‐total analysis systems
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