Ferritin Protein Regulates the Degradation of Iron Oxide Nanoparticles |
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| Authors: | Jeanne Volatron Florent Carn Jelena Kolosnjaj‐Tabi Yasir Javed Quoc Lam Vuong Yves Gossuin Christine Ménager Nathalie Luciani Gaëlle Charron Miryana Hémadi Damien Alloyeau Florence Gazeau |
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| Affiliation: | 1. Laboratoire Matières et Systèmes Complexes, UMR 7057 CNRS/Université Paris Diderot, Sorbonne Paris Cité, Paris Cedex 13, France;2. Laboratoire Matériaux et Phénomènes Quantiques, UMR 7162 CNRS/Université Paris Diderot, Sorbonne Paris Cité, Paris Cedex 13, France;3. Department of Physics, University of Agriculture, Faisalabad, Pakistan;4. Service de Physique Biomédicale, Université de Mons, Mons, Belgium;5. Laboratoire PHENIX, UMR 7195, CNRS/Université Pierre et Marie Curie/ESPCI, Paris Cedex 05, France;6. ITODYS, Interfaces, Traitements, Organisation et Dynamique des Systèmes, UMR 7086 CNRS/Université Paris Diderot, Sorbonne Paris Cité, Paris Cedex 13, France |
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| Abstract: | Proteins implicated in iron homeostasis are assumed to be also involved in the cellular processing of iron oxide nanoparticles. In this work, the role of an endogenous iron storage protein—namely the ferritin—is examined in the remediation and biodegradation of magnetic iron oxide nanoparticles. Previous in vivo studies suggest the intracellular transfer of the iron ions released during the degradation of nanoparticles to endogenous protein cages within lysosomal compartments. Here, the capacity of ferritin cages to accommodate and store the degradation products of nanoparticles is investigated in vitro in the physiological acidic environment of the lysosomes. Moreover, it is questioned whether ferritin proteins can play an active role in the degradation of the nanoparticles. The magnetic, colloidal, and structural follow‐up of iron oxide nanoparticles and proteins in lysosome‐like medium confirms the efficient remediation of potentially harmful iron ions generated by nanoparticles within ferritins. The presence of ferritins, however, delays the degradation of particles due to a complex colloidal behavior of the mixture in acidic medium. This study exemplifies the important implications of intracellular proteins in processes of degradation and metabolization of iron oxide nanoparticles. |
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| Keywords: | biodegradation ferritin iron oxide nanoparticles iron metabolism nanomagnetism |
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