| Lactobacillus diolivorans二醇脱水酶的分离纯化与酶学性质 |
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| 引用本文： | 孟晓蕾,齐向辉,韦宇拓,黄日波.Lactobacillus diolivorans二醇脱水酶的分离纯化与酶学性质[J].化工学报,2006,57(12):2938-2942. |
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| 作者姓名： | 孟晓蕾 齐向辉 韦宇拓 黄日波 |
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| 作者单位： | 广西大学广西亚热带生物资源保护利用重点实验室，广西 南宁 530004;南宁中诺生物工程公司，广西 南宁 530003 ; |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 引 言 1,3-丙二醇(1,3-propanediol, 1,3-PD)是一种重要的化工和医药中间体原料,是良好的溶剂、抗冻剂或保护剂,是聚酯--聚对苯二甲酸丙二醇酯(PTT)和聚氨酯的单体,可在地毯、纺织、工程塑料等领域中广泛应用,也可用于食品、化妆品和制药等行业食品.当前化工合成是生产1,3-丙二醇的主要方法,但需要在高温和昂贵催化剂作用下进行,并有多种副产物产生,产品分离困难,生产成本高,产生的废气对环境污染大.生物法较化学合成法具有条件温和、操作简便、副产物少、无环境污染等特点,因此如何用微生物发酵法低成本低污染地制备1,3-丙二醇已经成为各国研发者感兴趣的课题.
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| 关 键 词： | Lactobacillus diolivorans 二醇脱水酶 辅酶B12 甘油脱水酶 |
| 文章编号： | 0438-1157（2006）12-2938-05 |
| 收稿时间： | 2006-03-28 |
| 修稿时间： | 2006-03-282006-05-22 |
Purification and characterization of diol dehydratase of Lactobacillus diolivorans |
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| MENG Xiaolei,QI Xianghui,WEI Yutuo,HUANG Ribo.Purification and characterization of diol dehydratase of Lactobacillus diolivorans[J].Journal of Chemical Industry and Engineering(China),2006,57(12):2938-2942. |
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| Authors: | MENG Xiaolei QI Xianghui WEI Yutuo HUANG Ribo |
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| Affiliation: | Guangxi Provincial Key Laboratory of Subtropical Bioresource Conservation and Utilization, Guangxi University, Nanning 530004, Guangxi, China; Sinozyme Biotech. Co., Ltd. , Nanning 530003, Guangxi, China |
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| Abstract: | Diol dehydratase of Lactobacillus diolivorans was purified by ammonium sulfate precipitation and gel filtration chromatography.The molecular mass of the native complex was estimated to be 200×103 by nondenaturing polyacrylamide gel electrophoresis.Three main bands were present after SDS-PAGE with molecular masses of 62×103, 35×103 and 22×103, respectively. Km for the three main substrates were 1.3 mmol·L-1 for 1,2-propanediol，5.8 mmol·L-1 for glycerol and 10.3 mmol·L-1 for 1,2-ethanediol.Km for adenosylcobalamin coenzyme was 1.5 μmol·L-1, which was required for the enzyme catalysis.The optimal reaction conditions of the enzyme were pH 8.6 and 37℃. |
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| Keywords: | Lactobacillus diolivorans diol dehydratase coenzyme B12 glycerol dehydratase |
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