| 一种通过重组大肠杆菌和重组恶臭假单胞菌生产3-羟基癸酸的新方法 |
| |
| 引用本文： | 郑重, ,宫强, ,陈国强.一种通过重组大肠杆菌和重组恶臭假单胞菌生产3-羟基癸酸的新方法[J].中国化学工程学报,2004,12(4):550-555. |
| |
| 作者姓名： | 郑重 宫强 陈国强 |
| |
| 作者单位： | DepartmentofBiologicalScienceandBiotechnology,TsinghuaUniversity,Beijing100084,China |
| |
| 摘 要： | 3-hydroxydecanoic acid (3HD) is an interesting intermediate for chemical synthesis of many valuable compounds. A novel method to produce 3HD by recombinant bacteria was constructed in Escherichia coli HB101 and Pseudomonas putida GPp104, respectively. Simultaneous expression of both phaG encoding (R)-3-hydroxydecanoyl-ACP:CoA transacylase and tesB encoding thioesterase Ⅱ in E. coli HB101 increased 3HD production approximate 1.7-folds compared with the expression of phaG gene alone under identical conditions. In addition, when the tesB gene was introduced into the strain, the polyhydroxyalkanoate synthase negative strain P. putida GPpl04 produced extracellular 3HD. Thus, a novel pathway to produce 3HD by recombinant Pseudomonas was constructed. It was also found that the ratio of carbon source to nitrogen source affected the production of 3HD by recombinant P.putida harboring tesB gene. Nitrogen limitation seemed to promote the extracellular 3HD production.
|
| 关 键 词： | thioesterase Ⅱ |
| 修稿时间： | |
A Novel Method for Production of 3-Hydroxydecanoic Acid by Recombinant Escherichia coli and Pseudomonas putida |
| |
| ZHENG Zhong,GONG Qiang,CHEN Guoqiang.A Novel Method for Production of 3-Hydroxydecanoic Acid by Recombinant Escherichia coli and Pseudomonas putida[J].Chinese Journal of Chemical Engineering,2004,12(4):550-555. |
| |
| Authors: | ZHENG Zhong GONG Qiang CHEN Guoqiang |
| |
| Affiliation: | Department of Biological Science and Biotechnology, Tsinghua University, Beijing 100084, China |
| |
| Abstract: | 3-hydroxydecanoic acid (3HD) is an interesting intermediate for chemical synthesis of many valuable compounds. A novel method to produce 3HD by recombinant bacteria was constructed in Escherichia coli HB101 and Pseudomonas putida GPp104, respectively. Simultaneous expression of both phaG encoding (R)-3-hydroxydecanoylACP:CoA transacylase and tesB encoding thioesterase Ⅱ in E. coli HB101 increased 3HD production approximate1.7-folds compared with the expression of phaG gene alone under identical conditions. In addition, when the tesB gene was introduced into the strain, the polyhydroxyalkanoate synthase negative strain P. putida GPp104 produced extracellular 3HD. Thus, a novel pathway to produce 3HD by recombinant Pseudomonas was constructed. It was also found that the ratio of carbon source to nitrogen source affected the production of 3HD by recombinant P.putida harboring tesB gene. Nitrogen limitation seemed to promote the extracellular 3HD production. |
| |
| Keywords: | 3-Hydroxydecanoic acid polyhydroxyalkanoates Escherichia coli Pseudomonas putida (R)-3-hydroxydecanoyl-ACP:CoA transacylase PHB |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《中国化学工程学报》浏览原始摘要信息 |
|
点击此处可从《中国化学工程学报》下载全文 |
