| 用于清除胆红素的磁性亲和分离方法 |
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| 引用本文： | 徐辉,张国亮,张凤宝,王淑兰.用于清除胆红素的磁性亲和分离方法[J].中国化学工程学报,2003,11(4):456-459. |
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| 作者姓名： | 徐辉 张国亮 张凤宝 王淑兰 |
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| 作者单位： | SchoolofChemicalEngineeringandTechnology,TianjinUniversity,Tianjin300072,China |
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| 基金项目： | Supported by the National Natural Science Foundation of China (No. 29776036). |
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| 摘 要： | An affinity adsorbent, Cibacron Blue 3GA immobilized magnetic polyvinyl alcohol (PVA) microspheres was used for bilirubin removal taking the advantage of easy separation of magnetic sorbent from the biosystem.Fe3 O4 superparamagnetic particles was synthesized with hydrothermal reaction of ferrous chloride (FeC12) and ferric chloride (FeCl3). Such magnetic particles are then encapsulated in biocompatible PVA to form magnetic polymer microspheres sized from 2 to 15 nm with hydroxyl groups on its surface. Cibacron Blue 3GA, a dye-ligand, was covalently coupled with the polyvinyl alcohol through the nucleophilic reaction between the chloride of its triazine ring and the hydroxyl groups of PVA molecules under alkaline condition. The affinity adsorbent carried 21.1μmol Cibacron Blue 3GA per gram magnetic polymer microspheres was used to remove unconjugated and conjugated bilirubin from the solution which was composed of bilirubin or bilirubin and protein. After the adsorption, the adsorbent loaded with bilirubin was removed easily in the magnetic field.
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| 关 键 词： | 胆红素 磁性亲和分离 分离方法 血色素 磁性吸附剂 FeCl3 聚氯乙烯 |
| 修稿时间： | |
Study on Removal of Bilirubin with Magnetic Affinity Separation Technique |
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| XU Hui,ZHANG Guoliang,ZHANG Fengbao,WANG Shulan.Study on Removal of Bilirubin with Magnetic Affinity Separation Technique[J].Chinese Journal of Chemical Engineering,2003,11(4):456-459. |
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| Authors: | XU Hui ZHANG Guoliang ZHANG Fengbao WANG Shulan |
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| Affiliation: | School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China |
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| Abstract: | An affinity adsorbent, Cibacron Blue 3GA immobilized magnetic polyvinyl alcohol (PVA) |
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| Keywords: | Cibacron Blue 3GA bilirubin magnetic affinity separation |
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