| 发酵液中乳酸的盐析萃取 |
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| 引用本文： | 魏搏超,宋志远,孙亚琴,修志龙.发酵液中乳酸的盐析萃取[J].过程工程学报,2012,12(1):44-48. |
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| 作者姓名： | 魏搏超 宋志远 孙亚琴 修志龙 |
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| 作者单位： | 大连理工大学生命科学与技术学院,辽宁大连,116024 |
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| 摘 要： | 研究了一种利用盐析萃取法分离发酵液中乳酸的新方法. 通过系统考察乳酸在不同盐析萃取体系中的分配规律，发现K2HPO4-甲醇和K2HPO4-乙醇体系适合分离发酵液中的乳酸. 发酵液中乳酸浓度为167 g/L时，采用25%(w) K2HPO4-26%(w)甲醇盐析萃取体系，乳酸的分配系数和回收率分别为4.01和86.0%；采用14%(w) K2HPO4-30%(w)乙醇盐析萃取体系，乳酸的分配系数和回收率分别为3.23和90.6%. 此时上相中残余葡萄糖、菌体和可溶性蛋白的去除率分别达67.3%, 100%和85.9%.
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| 关 键 词： | 乳酸 盐析萃取 发酵液 |
| 收稿时间： | 2011-7-22 |
| 修稿时间： | 2012-1-10 |
Salting-out Extraction of Lactic Acid from Fermentation Broth |
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| WEI Bo-chao
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SONG Zhi-yuan
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SUN Ya-qin
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XIU Zhi-long.Salting-out Extraction of Lactic Acid from Fermentation Broth[J].Chinese Journal of Process Engineering,2012,12(1):44-48. |
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| Authors: | WEI Bo-chao SONG Zhi-yuan SUN Ya-qin XIU Zhi-long |
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| Affiliation: | (School of Life Science and Biotechnology,Dalian University of Technology,Dalian,Liaoning 116024,China) |
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| Abstract: | Salting-out extraction was used for recovery of lactic acid from fermentation broth.The different hydrophilic organic solvents-inorganic salts systems were investigated for their extraction ability.The results showed that the K2HPO4-methanol and K2HPO4-ethanol systems appeared to be favorable.The partition coefficient and recovery rate of lactic acid reached up to 4.01 and 86.0% for the system of 25%(ω) K2HPO4-26%(ω) methanol,when the concentration of lactic acid was 167 g/L in the broth.For the system of 14%(ω) K2HPO4-30%(ω) ethanol,the partition coefficient and recovery rate of lactic acid reached up to 3.23 and 90.6%.The removal rate of glucose,cells and proteins reached 67.3%,100% and 85.9%,respectively. |
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| Keywords: | lactic acid salting-out extraction fermentation broth |
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