Ratiometric Interactions of Anionic Surfactants with Calf Thymus DNA Bound Cationic Surfactants: Study II |
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| Authors: | Tandrima Chaudhuri Animesh Pan Suman Das Satya Priya Moulik |
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| Affiliation: | 1. Department of Chemistry, Dr. Bhupendranath Dutta Smriti Mahavidyalaya, Burdwan, India;2. Centre for Surface Science, Department of Chemistry, Jadavpur University, Kolkata, India |
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| Abstract: | Cationic surfactant cetyltrimethylammonium bromide (CTAB) and cetylpyridinium bromide (CPB) bind to the calf thymus DNA (ct‐DNA) like anionic biopolymers electrostatically, and establish equilibrium in aqueous medium at pH 7. At low concentration, ct‐DNA does not interact with anionic surfactants, sodium dodecylsulfate (SDS) and sodium dodecylbenzylsulfonate (SDBS). However, in the ground state, anionic surfactant is found to clearly establish equilibrium with ct‐DNA‐bound cationic surfactant whereby the same surfactant–DNA isosbestic point reappears. We herein report a detail ratiometric binding of CPB with ct‐DNA, and interaction of anionic SDBS with DNA‐bound CPB in comparison with the combined ct‐DNA–CTAB–SDS system. Compaction of ct‐DNA in presence of CPB and its decompaction using anionic SDBS is also studied in comparison with CTAB–SDS combination. The techniques used are tensiometry, spectrophotometry, viscometry, cyclic voltammetry, circular dichroism, isothermal titration calorimetry, and density functional theory (DFT)‐based computational calculations. The size and surface charge density of the surfactant headgroups and the phosphate group in DNA have a contributing role in the DNA compaction–decompaction phenomenon. |
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| Keywords: | DNA compaction– decompaction Cationic and anionic surfactant combination CPB– SDBS CTAB– SDS Electrostatic interaction |
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