Hybrid Paper–Plastic Microchip for Flexible and High‐Performance Point‐of‐Care Diagnostics |
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| Authors: | Mohamed Shehata Draz Maryam Moazeni Manasa Venkataramani Harini Lakshminarayanan Ecem Saygili Nivethitha Kota Lakshminaraasimulu Kamyar Mehrabi Kochehbyoki Manoj Kumar Kanakasabapathy Shirin Shabahang Anish Vasan Mohamad Ali Bijarchi Adnan Memic Hadi Shafiee |
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| Affiliation: | 1. Division of Engineering in Medicine, Department of Medicine, Brigham and Women's, Hospital, Harvard Medical School, Boston, MA, USA;2. Department of Medicine, Harvard Medical School, Boston, MA, USA;3. Faculty of Science, Tanta University, Tanta, Egypt;4. Center for Nanotechnology, King Abdulaziz University, Jeddah, Saudi Arabia |
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| Abstract: | A low‐cost and easy‐to‐fabricate microchip remains a key challenge for the development of true point‐of‐care (POC) diagnostics. Cellulose paper and plastic are thin, light, flexible, and abundant raw materials, which make them excellent substrates for mass production of POC devices. Herein, a hybrid paper–plastic microchip (PPMC) is developed, which can be used for both single and multiplexed detection of different targets, providing flexibility in the design and fabrication of the microchip. The developed PPMC with printed electronics is evaluated for sensitive and reliable detection of a broad range of targets, such as liver and colon cancer protein biomarkers, intact Zika virus, and human papillomavirus nucleic acid amplicons. The presented approach allows a highly specific detection of the tested targets with detection limits as low as 102 ng mL?1 for protein biomarkers, 103 particle per milliliter for virus particles, and 102 copies per microliter for a target nucleic acid. This approach can potentially be considered for the development of inexpensive and stable POC microchip diagnostics and is suitable for the detection of a wide range of microbial infections and cancer biomarkers. |
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| Keywords: | electrical sensing flexible electronics paper microfluidics plastic microfluidics point‐of‐care diagnostics |
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