| 蛋白质剪接在蛋白质研究和蛋白质工程中的应用 |
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| 引用本文： | 戴旭东,孟清,刘相钦.蛋白质剪接在蛋白质研究和蛋白质工程中的应用[J].自然杂志,2012,34(1):32-38. |
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| 作者姓名： | 戴旭东 孟清 刘相钦 |
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| 作者单位： | 1.博士研究生，2教授，东华大学生物科学与技术研究所,上海201620;3.教授，Department of Biochemistry & Molecular Biology, Faculty of Medicine, Dalhousie University, Halifax, Nova Scotia, Canada B3H1X5 |
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| 摘 要： | 蛋白质剪接(protein splicing)是由蛋白质内含子介导的在蛋白质水平上翻译后的加工过程。蛋白质内含子(intein)是指前体蛋白中的一段插入序列，它在蛋白质翻译后的成熟过程中能自我催化，使自身从前体蛋白中切除，并将其两侧的多肽片段以肽键连接，形成成熟的蛋白质。蛋白质内含子的发现丰富了基因表达和蛋白质翻译成熟过程的理论，而且在蛋白质研究和蛋白质工程中有广泛的应用。本文试图综述蛋白质剪接在蛋白质特异位点标记、蛋白质片段化标记同位素、蛋白质环化、蛋白质芯片、基因治疗等研究中的应用。
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| 关 键 词： | 蛋白质剪接 断裂蛋白质内含子 蛋白质研究 蛋白质工程 |
| 收稿时间： | 2011-09-30 |
| 修稿时间： | 2011-10-30 |
Protein Splicing Technology in Protein Research and Protein Engineering |
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| DAI Xu-dong,MENG Qing,LIU Xiang-qin.Protein Splicing Technology in Protein Research and Protein Engineering[J].Chinese Journal of Nature,2012,34(1):32-38. |
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| Authors: | DAI Xu-dong MENG Qing LIU Xiang-qin |
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| Affiliation: | 1.Ph. D. Candidate， 2Professor, Institute of Biological Science and Technology, Donghua University, Shanghai 201620, China; 3.Professor，Department of Biochemistry & Molecular Biology, Faculty of Medicine, Dalhousie University, Halifax, Nova Scotia, Canada B3H1X5 |
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| Abstract: | Protein splicing is post-translational process mediated by inteins.Inteins are internal protein sequences that self-excise and splice together the flanking sequences with a peptide bond.The discovery of inteins enhances theories of gene expression and post-translation,protein splicing technology can be applied in protein research and protein engineering.This review provides a survey of recent applications of protein splicing including protein labeling,protein segmental isotope-labeling,protein cyclization,protein chip,gene therapy and so on. |
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| Keywords: | protein splicing split protein protein research protein engineering |
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