| 灯盏细辛联合依帕司他对糖尿病肾病大鼠肾皮质中CAT、SOD、MDA表达的影响 |
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| 引用本文： | 屈雅娟,张敬芳,孙旗,王雄,易文媛,陈贲,彭攀,郭承熙.灯盏细辛联合依帕司他对糖尿病肾病大鼠肾皮质中CAT、SOD、MDA表达的影响[J].中医药信息,2023,40(9):41-45. |
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| 作者姓名： | 屈雅娟 张敬芳 孙旗 王雄 易文媛 陈贲 彭攀 郭承熙 |
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| 作者单位： | 长沙医学院第一临床学院,长沙医学院第一临床学院,长沙医学院第一临床学院,长沙医学院第一临床学院,长沙医学院第一临床学院,长沙医学院第一临床学院,长沙医学院第一临床学院,长沙医学院第一临床学院 |
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| 基金项目： | 2021年国家级大学生创新创业训练计划项目,教高司函[2021]13号-一般项目-202110823010；湖南省普通高等学校教学改革研究项目:HNJG-2020-1038; |
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| 摘 要： | 【摘要】 目的：探究灯盏细辛对SD大鼠CAT、SOD、MDA表达的影响的及抗氧化应激的作用。方法：将50只大鼠随即平均分为正常对照组、模型组、各给药组（灯盏细辛组、依帕司他组以及联合组）。除正常对照组，其余各组采用喂养高脂高糖饲料辅助一次性注射链脲佐菌素（STZ）建立糖尿病肾病模型大鼠。建模成功后，持续灌胃给药６周，对造模前与治疗过程中各组大鼠的体质量、血糖以及24h微量白蛋白(24hUmALb)含量进行记录和比较；给药6周后处死大鼠，检测肾皮质中过氧化氢酶（CAT）活性采用钼酸铵法，检测大鼠肾皮质中超氧化物歧化酶（SOD）活性采用WST1法，以及检测肾皮质中丙二醛（MDA）含量采用TBA法。结果：治疗后6周，与模型组相比，联合组大鼠体质量与模型组相比，上升最显著（P<0.01），其次是依帕司他组，最后是灯盏细辛组，联合组与依帕司他组和灯盏细辛组相比具有统计学意义（P<0.05）。治疗后6周，联合组的血糖，24hUmALb水平显著低于模型组/依帕司他组和灯盏细辛组（P<0.05）。治疗后6周，各组大鼠肾皮质氧化应激指标CAT、SOD和MDA水平比较，联合组大鼠肾皮质CAT和SOD水平明显高于模型组、依帕司他组和灯盏细辛组（P<0.05），MDA水平明显低于模型组、依帕司他组和灯盏细辛组（P<0.05）。结论：灯盏细辛联合依帕司他能有效纠正糖尿病肾病肾损伤和延缓病情发展，其可能与其上调CAT、SOD活性，降低MDA含量进而调控糖尿病肾病肾组织氧化应激有关。
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| 关 键 词： | 糖尿病肾病 灯盏细辛 依帕司他 过氧化物酶 超氧化物歧化酶 丙二醛 |
| 收稿时间： | 2023/3/27 0:00:00 |
| 修稿时间： | 2023/9/2 0:00:00 |
Experimental study on the effect of Erigeron breviscapus on the expression of CAT, SOD, MDA and antioxidant stress in SD rats |
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| QuYaJuan,zhangjingfang,sunqi,wangxiong,yiwengyuan,chenben,pengpan and guochengxi.Experimental study on the effect of Erigeron breviscapus on the expression of CAT, SOD, MDA and antioxidant stress in SD rats[J].Information on Traditional Chinese Medicine,2023,40(9):41-45. |
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| Authors: | QuYaJuan zhangjingfang sunqi wangxiong yiwengyuan chenben pengpan and guochengxi |
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| Abstract: | Abstract] Objective: Exploring the effect of Erigeron breviscapus on the expression of CAT, SOD, MDA in SD rats and the role of antioxidant stress. Methods: Fifty rats were randomly divided into normal control group, model group, and each administration group (Erigeron breviscapus group, eparastatin group, and combination group). In addition to the normal control group, the rats in the other groups were fed with high-fat and high-sugar diet supplemented with one-time injection of streptozotocin (STZ) to establish the model of diabetes nephropathy. After successful modeling, the rats were continuously administered by gavage for 6 weeks, and the body weight, blood glucose, and 24-hour microalbumin (24h UmALb) content of each group were recorded and compared before and during modeling and treatment; After 6 weeks of administration, the rats were killed. The activity of catalase (CAT) in the renal cortex was measured using the ammonium molybdate method, the activity of superoxide dismutase (SOD) in the renal cortex was measured using the WST1 method, and the content of malondialdehyde (MDA) in the renal cortex was measured using the TBA method. Results: At 6 weeks after treatment, compared with the model group, the weight of rats in the combined group increased most significantly compared to the model group (P<0.01), followed by the epalrestat group, and finally by the breviscapine group. The combined group had a statistically significant increase compared to the epalrestat group and the breviscapine group (P<0.05). At 6 weeks after treatment, the blood glucose and 24 h UmALb levels in the combined group were significantly lower than those in the model group/eparastatin group and the breviscapine group (P<0.05). After 6 weeks of treatment, the levels of CAT, SOD, and MDA in the renal cortex of rats in each group were compared. The levels of CAT and SOD in the renal cortex of rats in the combined group were significantly higher than those in the model group, eparasta group, and breviscapine group (P<0.05), while the levels of MDA were significantly lower than those in the model group, eparasta group, and breviscapine group (P<0.05). Conclusion: Erigeron breviscapus combined with epalrestat can effectively correct the renal injury of diabetes nephropathy and delay the development of the disease, which may be related to the up regulation of CAT and SOD activities, the reduction of MDA content and the regulation of oxidative stress in renal tissue of diabetes nephropathy. |
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| Keywords: | diabetes nephropathy Erigeron breviscapus Ipalestat Peroxidase Superoxide dismutase Malondialdehyde |
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