| lncRNA TUG1在结直肠癌组织和细胞中的表达及其对癌细胞增殖、侵袭能力的影响 |
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| 引用本文： | 金益峰,周 勇,王厚明,骆 洋,钟 鸣,崔 荣.lncRNA TUG1在结直肠癌组织和细胞中的表达及其对癌细胞增殖、侵袭能力的影响[J].现代肿瘤医学,2023,0(12):2270-2274. |
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| 作者姓名： | 金益峰 周 勇 王厚明 骆 洋 钟 鸣 崔 荣 |
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| 作者单位： | 1.上海市嘉定区中医医院普外科，上海 201800;2.上海交通大学医学院附属仁济医院胃肠外科，上海 200127 |
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| 基金项目： | 上海市嘉定区农业和社会事业科研项目（编号:JDKW-2019-W22） |
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| 摘 要： | 目的:探讨lncRNA牛磺酸上调基因1（taurine upregulated gene 1，TUG1)与结直肠癌临床病理因素的相关性及临床意义，以及其促进结直癌细胞增殖和侵袭的生物学功能。方法:实时定量PCR（real-time quantitative PCR,RT-qPCR）检测结直肠癌组织TUG1的表达水平，并采用卡方检验分析TUG1表达水平与临床资料的相关性。Kaplan-Meier生存曲线和log-rank检验分析TUG1表达水平与结直肠癌预后的相关性。CCK8实验和Transwell实验检测过表达和敲低TUG1后的结直肠癌细胞增殖或侵袭能力的变化。结果:TUG1在结直肠癌组织中表达水平明显升高（P<0.05）；TUG1表达水平与肿瘤大小、CEA水平、淋巴结转移、远处转移以及TNM分期密切相关（P<0.05）。Kaplan-Meier生存曲线和log-rank检验提示，TUG1表达水平越高，结直肠癌患者预后越差（P<0.05）。CCK8和Transwell实验结果表明，与对照组相比，pcDNA3.1-TUG1组结直肠癌细胞中TUG1表达水平明显增高(P＜0.05)，细胞增殖活性和侵袭数目增多(P＜0.05)；而siRNA-TUG1组结直肠癌细胞中TUG1表达水平明显降低(P＜0.05)，细胞增殖活性和侵袭数目减少(P＜0.05)。结论:TUG1在结直肠癌组织中呈高表达，且TUG1表达水平越高，患者预后越差。并且，lncRNA TUG1促进结直肠癌细胞的增殖和侵袭能力。
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| 关 键 词： | 结直肠癌 TUG1 增殖 侵袭 |
Expression of lncRNA TUG1 in colorectal cancer tissues and cells and its effect on the proliferation and invasion of cancer cells |
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| JIN Yifeng,ZHOU Yong,WANG Houming,LUO Yang,ZHONG Ming,CUI Rong.Expression of lncRNA TUG1 in colorectal cancer tissues and cells and its effect on the proliferation and invasion of cancer cells[J].Journal of Modern Oncology,2023,0(12):2270-2274. |
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| Authors: | JIN Yifeng ZHOU Yong WANG Houming LUO Yang ZHONG Ming CUI Rong |
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| Affiliation: | 1.General Surgery，Jiading Hospital of Traditional Chinese Medicine，Shanghai 201800,China;2.Gastrointestinal Surgery，Renji Hospital of Shanghai Jiao Tong University School of Medicine，Shanghai 200127,China. |
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| Abstract: | Objective:To analyze the lncRNA TUG1 correlation with clinicopathological characteristics，and to explore the effects of lncRNA TUG1 on the proliferation and invasion of colorectal cancer cells.Methods:Real-time quantitative PCR(RT-qPCR) was used to detect the expression level of TUG1 in colorectal cancer.Chi-square test was used to analyze the correlation between TUG1 expression level and clinical data.Kaplan-Meier survival curve and log-rank test were used to analyze the correlation between TUG1 expression level and prognosis of colorectal cancer.CCK8 experiment and Transwell experiment were used to detect the changes of proliferation or invasion ability of colorectal cancer cells after overexpression and knockdown of TUG1.Results:The expression level of TUG1 in colorectal cancer was significantly increased（P<0.05）. The expression level of TUG1 is closely related to tumor size,CEA level,lymph node metastasis,distant metastasis and TNM staging（P<0.05）.Kaplan-Meier survival curve and log-rank test indicated that the higher the expression level of TUG1, the worse the prognosis of patients with colorectal cancer（P<0.05）.The results of CCK8 and Transwell experiments showed that compared with the control group,the expression level of TUG1 in colorectal cancer cells in pcDNA3.1-TUG1 group increased significantly（P<0.05）,and the cell proliferation activity and invasion number increased（P<0.05）.The expression level of TUG1 in colorectal cancer cells in siRNA-TUG1 group decreased significantly（P<0.05）,and the cell proliferation activity and invasion number decreased（P<0.05）.Conclusion:TUG1 is highly expressed in colorectal cancer tissues,and the higher the expression level of TUG1,the worse the prognosis of patients.Furthermore,lncRNA TUG1 promotes the proliferation and invasion of colorectal cancer cells. |
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| Keywords: | colorectal cancer TUG1 proliferation invasion |
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