| 贝类产品中软骨藻酸的HPLC检测方法 |
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| 引用本文： | 高治昊,林郑忠,陈晓梅,黄志勇.贝类产品中软骨藻酸的HPLC检测方法[J].集美大学学报(自然科学版),2015,0(4):255-259. |
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| 作者姓名： | 高治昊 林郑忠 陈晓梅 黄志勇 |
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| 作者单位： | （集美大学食品与生物工程学院，福建 厦门 361021） |
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| 基金项目： | 国家自然科学基金资助项目，福建省科技计划重点项目，厦门市科技计划项目，集美大学创新团队基金资助项目 |
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| 摘 要： | 建立了高效液相色谱测定贝类产品中软骨藻酸的检测方法，具体过程为:样品先经甲醇浸提后，再用甲醇-水(V（甲醇）∶V（水）=1∶1）提取2次，合并提取液后用Zorbax SB300-C18柱(2.1 mm×250 mm，5 μm)进行分离，以乙腈-0.2%磷酸水溶液（含0.1%三乙胺）（V（乙腈）∶V（磷酸水溶液）=12∶88）为流动相于室温下等度洗脱，流速为0.25 mL/min，进样量20 μL，检测波长为242 nm.结果表明，在建立的提取方法和色谱条件下，软骨藻酸从复杂的样品基体中被分离出来，在0.02~1.0 mg/L质量浓度范围内具有良好的线性关系（r＞0.999），样品的平均加标回收率为108%，测量方法的RSD为3.5%（n=6），检出限为23.5 ng/g.所建立的检测方法可适用于对贝类产品中软骨藻酸的快速检测.
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| 关 键 词： | 软骨藻酸 高效液相色谱 贝类 快速检测 |
Rapid Detection of Domoic Acid in Shellfishes by HPLC |
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| GAO Zhi-hao,LIN Zheng-zhong,CHEN Xiao-mei,HUANG Zhi-yong.Rapid Detection of Domoic Acid in Shellfishes by HPLC[J].the Editorial Board of Jimei University(Natural Science),2015,0(4):255-259. |
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| Authors: | GAO Zhi-hao LIN Zheng-zhong CHEN Xiao-mei HUANG Zhi-yong |
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| Affiliation: | (College of Food and Biological Engineering，Jimei University，Xiamen 361021，China) |
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| Abstract: | A rapid detection method by high performance liquid chromatography (HPLC) was developed to determine the concentrations of domoic acid in different species of shellfish.After extraction by methanol and methanol-water (1∶1,V/V)，the sample was separated using a Zorbax SB300-C18 chromatographic column (2.1 mm×250 mm，5 μm)，and was eluted with acetonitrile-0.2% phosphoric acid solution (containing 0.5% triethylamine) (12∶88,V/V) at room temperature.The flow rate was set as 0.25 mL/min.20 μL of sample was individually injected，and the detection wavelength was 242 nm.The results showed that domoic acid can be well separated from the sample matrix within 8 min with a linear range of 0.02~1.0 mg/L(r＞0.999).The average recovery as tested by adding standards was 108%，and the RSD was 3.5%(n=6).The detection limit estimated with three folds of standard deviations was 23.5 ng/g.In conclusion,the established method can be used to detect trace domoic acid in shellfish samples rapidly without further purification treatment by SPE columns. |
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| Keywords: | domoic acid HPLC shellfish rapid detection |
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