| 痔消栓的HPLC含量测定 |
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| 引用本文： | 张荣,邓秀霞,卫罡,李润美,李文周,陈军.痔消栓的HPLC含量测定[J].中国实验方剂学杂志,2009,15(7):25-27. |
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| 作者姓名： | 张荣 邓秀霞 卫罡 李润美 李文周 陈军 |
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| 作者单位： | 1. 广州中医药大学,广东,广州,510405
2. 广东轻工职业技术学院,广东,广州,510300 |
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| 摘 要： | 目的:建立痔消栓的HPLC含量测定方法。方法:采用HPLC法,以KromasilC18(4.6mm×250mm,5μm)色谱柱为固定相,以乙腈-0.2%磷酸水溶液为流动相,梯度洗脱,流速1.0mL·min-1,柱温23℃,检测波长为203nm。结果:三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1分别在0.368～2.76μg,0.676～5.07μg,0.812～6.09μg范围内线性关系良好,相关系数分别为0.9999,0.9999,0.9998,平均加样回收率分别为98.53%,99.20%,99.35%,RSD分别为2.11%,1.88%,2.38%。结论:样品分离效果好,测定结果准确、可靠,重复性好,可用于痔消栓的质量控制。
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| 关 键 词： | 痔消栓 三七皂苷R1 人参皂苷Rg1 人参皂苷Rb1 高效液相色谱法 |
| 收稿时间： | 2009/2/25 0:00:00 |
HPLC Determination of Saponins in Zhixiao Suppositories |
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| ZHANG Rong,Deng Xiu-xi,WEI Gang,LI Run-mei,LI Wen-zhou,CHEN Jun.HPLC Determination of Saponins in Zhixiao Suppositories[J].China Journal of Experimental Traditional Medical Formulae,2009,15(7):25-27. |
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| Authors: | ZHANG Rong Deng Xiu-xi WEI Gang LI Run-mei LI Wen-zhou CHEN Jun |
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| Affiliation: | 1.Guangzhou University of Chinese Medicine,Guangzhou 510405,China;2.Guangdong Industry Technical College,Guangzhou 510300,China |
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| Abstract: | Objective: To denvolop HPLC an method for the determination of the content of three saponins in Zhixiao suppositories. Methods: Analysis carriedout was performed on a Kromasil C18 column (4.6 mm×250 mm., 5μm)at 23℃. The mobile phases were methanol and 0.2% phosphoricacid solution in a gradient elution. The flow rate was set at 1.0 mL·min-1 and the ultraviolet detector was operated at 203 nm. Results: The linear ranges of notoginsenoside R1, ginsenoside Rg1 and ginsenoside Rb1 were 0.368～2.76μg,0.676～5.07 μg and 0.812～6.09μg,respectively. The average recoveries of the three saponins were 98.53%, 99.20% and 99.35%, respectively. The RSD (n=5) were 2.11%, 1.88% and 2.38%, respectively. Conclusion: The separation is a good and the results are accurate and reproducible. It can be used for the quality control of Zhixiao suppositories. |
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| Keywords: | Zhixiao suppositories notoginsenoside R1 ginsenoside Rg1 ginsenoside Rb1 HPLC |
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