| LncRNA LINC00958通过靶向miR-490-3p促进甲状腺乳头状癌细胞的增殖、侵袭和迁移 |
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| 引用本文： | 袁 宁,王晓娟,白亚君,何明海,王 凤.LncRNA LINC00958通过靶向miR-490-3p促进甲状腺乳头状癌细胞的增殖、侵袭和迁移[J].现代肿瘤医学,2023,0(10):1785-1793. |
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| 作者姓名： | 袁 宁 王晓娟 白亚君 何明海 王 凤 |
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| 作者单位： | 1.南充市中心医院内分泌科;2.感控科，四川 南充 637003 |
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| 基金项目： | 四川省南充市市校科技战略合作课题（编号:19SXHZ0173） |
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| 摘 要： | 目的:研究LncRNA LINC00958对甲状腺乳头状癌细胞的影响及其作用机制。方法:TPC-1和K-1细胞分别分成Control、shRNA-NC、shRNA-LINC00958-1、shRNA-NC+inhibitor-NC、shRNA-LINC00958-1+inhibitor-NC和shRNA-LINC00958-1+miR-490-3p inhibitor组。TPC-1和K-1细胞移植的荷瘤鼠分别分为shRNA-NC、shRNA-LINC00958-1、shRNA-NC+inhibitor-NC、shRNA-LINC00958-1+inhibitor-NC和shRNA-LINC00958-1+miR-490-3p inhibitor组。用实时荧光定量PCR(RT-qPCR)检测LINC00958和miR-490-3p的表达水平;CCK-8检测细胞活力;Western blot检测蛋白表达水平;细胞划痕实验检测细胞迁移水平，Transwell小室检测细胞侵袭水平，克隆形成实验检测细胞增殖水平;双荧光素酶报告实验检测LINC00958和miR-490-3p的靶向关系。测定肿瘤质量及体积。结果:相比于正常组织，LINC00958在甲状腺癌组织中高表达;相比于Nthy-ori 3-1细胞，LINC00958在TPC-1和K-1细胞中高表达，miR-490-3p低表达。LINC00958沉默后，甲状腺乳头状癌细胞的增殖、侵袭和迁移能力显著降低。干扰miR-490-3p表达逆转LINC00958沉默对甲状腺乳头状癌细胞的增殖、迁移和侵袭效果，破坏LINC00958沉默对肿瘤生长的抑制效果。结论:抑制LINC00958表达可抑制TPC-1和K-1细胞增殖、迁移和侵袭能力，其机制与靶向miR-490-3p表达有关。
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| 关 键 词： | LncRNA LINC00958 miR-490-3p 甲状腺乳头状癌 细胞迁移 细胞侵袭 |
LncRNA LINC00958 promotes the proliferation,invasion and migration of papillary thyroid carcinoma cells by targeting miR-490-3p |
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| YUAN Ning,WANG Xiaojuan,BAI Yajun,HE Minghai,WANG Feng.LncRNA LINC00958 promotes the proliferation,invasion and migration of papillary thyroid carcinoma cells by targeting miR-490-3p[J].Journal of Modern Oncology,2023,0(10):1785-1793. |
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| Authors: | YUAN Ning WANG Xiaojuan BAI Yajun HE Minghai WANG Feng |
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| Affiliation: | 1.Department of Endocrinology;2.Department of Sensory Control,Nanchong Central Hospital,Sichuan Nanchong 637003,China. |
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| Abstract: | Objective:To study the effect and its mechanism of lncRNA LINC00958 on thyroid cancer cells.Methods:TPC-1 and K-1 cells were divided into Control,shRNA-NC,shRNA-LINC00958-1,shRNA-NC+inhibitor-NC,shRNA-LINC00958-1+inhibitor-NC and shRNA-LINC00958-1+miR-490-3p inhibitor,respectively.Tumor-bearing mice with implantation of TPC-1 or K-1 cells were divided into shRNA-NC,shRNA-LINC00958-1,shRNA-NC+inhibitor-NC,shRNA-LINC00958-1+inhibitor-NC and shRNA-LINC00958-1+miR-490-3p inhibitor.The expression levels of LINC00958 and miR-490-3p were detected by real-time fluorescence quantitative PCR (RT-qPCR).Cell viability was detected by CCK-8 assay and Western blot was used to detect protein expression level.The migration and invasion of TPC-1 and K-1 cells were detected by wound healing and Transwell assay,respectively.Cell proliferation was detected by clone formation assay.The targeting relationship between LINC00958 and miR-490-3p was detected by dual luciferase reporting assay.The mass and volume of tumor were measured.Results:The results showed that compared with normal tissues,LINC00958 was highly expressed in thyroid cancer tissues.miR-490-3p showed low expression in TPC-1 and K-1 cells compared with Nthy-ori 3-1 cells.After silencing LINC00958,the proliferation,invasion and migration of papillary thyroid cancer cells were significantly decreased.miR-490-3p silencing blunted the effect of LINC00958 silencing on these malignant behaviors in vitro and suppressing tumor growth.Conclusion:LINC00958 silencing can inhibit proliferation,migration and invasion of thyroid cancer cells,as well as tumor growth by targeting miR-490-3p. |
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| Keywords: | LncRNA LINC00958 miR-490-3p papillary thyroid cancer migration invasion |
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