| 口腔粘膜白斑组织中上皮细胞凋亡的实验研究 |
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| 引用本文： | 王勤涛,朱萧玲,周威,董广英,袁乃梅.口腔粘膜白斑组织中上皮细胞凋亡的实验研究[J].华西口腔医学杂志,2003,21(1):7-9. |
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| 作者姓名： | 王勤涛 朱萧玲 周威 董广英 袁乃梅 |
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| 作者单位： | 710032 第四军医大学口腔医院牙周粘膜病科 |
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| 基金项目： | 国家自然科学基金资助项目 (编号 3970 0 0 35) |
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| 摘 要： | 目的 观察和比较口腔粘膜白斑(LK)病变和正常对照的局部活检粘膜组织中上皮细胞凋亡和增殖的情况。方法 应用原位末端转移酶标记技术和ABC免疫组化技术,通过组织学定位、相对染色强度和阳性细胞计数来评估上皮细胞凋亡和增殖细胞核抗原的变化程度及主要部位。结果 与对照相比,LK病变中凋亡阳性信号在棘细胞层较强,而增殖细胞核抗原则在基底细胞层及其周围呈现强阳性反应,病变组织固有层中浸润的部分炎细胞和血管内皮细胞也表达出较强的阳性凋亡信号。病变组和对照组阳性细胞计数量有显著性差异(P<0·05)。结论 白斑病变时上皮层棘细胞凋亡增强,而基底细胞的增殖能力活跃,该处局部组织的免疫防御能力可能有所下降。
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| 关 键 词： | 细胞凋亡 口腔粘膜白斑 原位末端转移酶标记技术 增殖 |
| 收稿时间： | 2003-02-25 |
| 修稿时间： | 2000年8月28日 |
An Experimental Study of Apoptosis in Epithelia of Oral Leukoplakia |
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| WANG Qintao,ZHU Xiaoling,ZHOU Wei,et al..An Experimental Study of Apoptosis in Epithelia of Oral Leukoplakia[J].West China Journal of Stomatology,2003,21(1):7-9. |
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| Authors: | WANG Qintao ZHU Xiaoling ZHOU Wei |
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| Affiliation: | Department ofPeriodontology and Oral Medicine,College ofStomatology,the Fourth MilitaryMedical University,Xi′an710032,China |
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| Abstract: | Objective\ The purpose of this study was to investigate the relationship between apoptosis and proliferation of oral mucosa in lesions of leukoplakia and the role of apoptosis in the pathogenesis of this common oral disease.Methods\ The mucosa was obtained from the leukoplakia lesions from 6 patients, with average age of 57 years, and all the patients had not received any treatment before this investigation. The lesions were located on the lip (1), gingiva (1), tongue (2) and buccal mucosa (2). All the patients did not have any systemic diseases. Other three normal oral mucosa tissues were collected as the control. In situ terminal deoxynucleotidyl transferase end_labeling (TUNEL) and avidin_biotin peroxidase complex (ABC) immunohistochemical assay were used to detect single_strand DNA breaks and proliferating cell nuclear antigens (PCNA). The negative control slides were treated with the tris saline buffer to substitute the terminal deoxynucleotidyl transferase (TdT) and PCNA_Ab in the assay. Five consecutive high power fields with the magnification of 400 were used to search for positive stained keratinocytes.Results\ Compared with the normal mucosa, the keratinocytes in leukoplakia showed positive apoptotic signals in the nucleus of suprabasal epithelial layers, while PCNA_positive stain was present in the basal position. In the connective tissue, positive apoptotic signals were found in lymphocytes and the endothelia of blood vessels.Conclusion\ The results indicate that the death of cells in leukoplakia is partly due to apoptosis which may play an important role in the genesis of oral leukoplakia. |
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| Keywords: | apoptosis \ oral mucosa leukoplakia \ terminal deoxynucleotidyl transferase end_labeling \ proliferation |
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