| 弓形弓形虫抗原检测方法的研究 |
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| 引用本文： | 李辉,许汴利,邓艳,赵旭东,蔺西萌,闫旭霞.弓形弓形虫抗原检测方法的研究[J].中国寄生虫学与寄生虫病杂志,2006,24(1):67-69. |
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| 作者姓名： | 李辉 许汴利 邓艳 赵旭东 蔺西萌 闫旭霞 |
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| 作者单位： | 河南省疾病预防控制中心,郑州,450003 |
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| 摘 要： | 本研究制备了与多种寄生虫抗原无交叉反应的抗弓形虫单抗B6C3和兔抗弓形虫多抗,多抗为捕获抗体,单抗为检测抗体,建立单-多抗夹心ELISA方法。用亲和素连接分别标记生物素的抗体和酶,建立ABC(avidin-biotin-peroxidase complex)-ELISAD1]方法。同样用亲和素连接标记生物素的抗体和DNA片段,利用PCR反应对DNA片段巨大的扩增能力,提高检测灵敏度,发展单-多抗夹心ELISA为免疫-PCR(I-PCR)检测弓形虫抗原。单-多抗夹心ELISA检测弓形虫抗原最低浓度为0.6μg/ml,ABC-ELISA检测最低浓度为0.075μg/ml。I-PCR检测最低浓度为0.1ng/ml,检测弓形虫抗原灵敏度高于传统ELISA法6000倍,高于ABC-ELISA法750倍。
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| 关 键 词： | 弓形虫 抗原 免疫测定 |
| 文章编号： | 1000-7423（2006）-01-0067-03 |
| 收稿时间： | 2005-03-17 |
| 修稿时间： | 2005-03-17 |
Study on the Methods for Detection of Toxoplasma Antigen |
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| LI Hui,XU Bian-li,DENG Yan,ZHAO Xu-dong,LIN Xi-meng,YAN Xu-xia.Study on the Methods for Detection of Toxoplasma Antigen[J].Chinese Journal of Parasitology and Parasitic Diseases,2006,24(1):67-69. |
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| Authors: | LI Hui XU Bian-li DENG Yan ZHAO Xu-dong LIN Xi-meng YAN Xu-xia |
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| Affiliation: | Henan Provincial Center for Disease Control and Prevention, Zhengzhou 450003, China |
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| Abstract: | A sandwich ELISA was established with monoclonal antibody as detecting antibody and rabbit anti-Toxoplasma polyclonal antibody as capturing antibody. ABC (avidin-biotin-peroxidase complex)-ELISA and immuno-PCR were also used to detect different concentrations of Toxoplasma antigen. The lowest concentration of antigen to be detected by sandwich ELISA, ABC-ELISA and immuno-PCR was 0. 6 microg/ml, 0. 075 microg/ml and 0.1 ng/ml respectively. The immuno-PCR shows a much higher sensitivity than the other two methods. |
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| Keywords: | Toxoplasma Antigen Immunoassay |
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