| 桦木酸对人胃癌SGC-7901细胞增殖的影响* |
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| 引用本文： | 陈亚楠,邵淑丽,何孟奇,黄鑫,张伟伟,张珍珠.桦木酸对人胃癌SGC-7901细胞增殖的影响*[J].中国应用生理学杂志,2020,36(6):628-632. |
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| 作者姓名： | 陈亚楠 邵淑丽 何孟奇 黄鑫 张伟伟 张珍珠 |
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| 作者单位： | 1.齐齐哈尔大学生命科学与农林学院, 齐齐哈尔 161000;2.抗性基因工程与寒地生物多样性保护黑龙江省重点实验室, 齐齐哈尔 161000 |
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| 基金项目： | *齐齐哈尔大学黑龙江省教育厅基本业务专项重点项目(135109104);黑龙江省高教强省优势特色学科—玉米“粮头食尾”重点专项(LTSW201737);黑龙江省省属高等学校基本科研业务费科研项目(植物性食品加工技术特色学科专项)(YSTSXK201809) |
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| 摘 要： | 目的:利用不同浓度的桦木酸对人胃癌SGC-7901细胞增殖的影响。方法:桦木酸设4个不同浓度(0、10、20、30 μg/ml),并采用常规化疗药物5-Fu处理作为阳性对照,以探究其对细胞增殖的影响。采用台盼蓝拒染法和吉姆萨染色法分别检测桦木酸对人胃癌SGC-7901细胞生长抑制率及克隆形成率;EdU法检测SGC-7901的细胞增殖;利用流式细胞术检测细胞周期, 应用qRT-PCR和Western blot分别检测细胞周期蛋白cyclin D1,cyclin B1的mRNA和蛋白表达水平。结果:不同浓度的桦木酸处理人胃癌SGC-7901细胞48 h后,其细胞生长抑制率显著升高(P＜0.05),克隆形成率和细胞增殖率均明显降低(P＜0.01),且呈剂量和时间依赖性;人胃癌SGC-7901细胞被阻滞在G1/G0期,细胞周期蛋白cyclin D1和cyclin B1的mRNA和蛋白表达量也随桦木酸浓度升高而显著降低(P＜0.01)。且与5-Fu对照组相比,桦木酸浓度为20 μg/ml和30 μg/ml时,细胞增殖能力明显降低,细胞周期被抑制,细胞周期蛋白表达量均明显降低(P ＜0.05)。结论:桦木酸通过下调cyclin B1和cyclin D1基因表达,将人胃癌SGC-7901细胞阻滞在G1/G0期,从而抑制细胞增殖。
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| 关 键 词： | 桦木酸 SGC-7901细胞 细胞增殖 细胞周期 |
| 收稿时间： | 2020-03-06 |
Effect of betulinic acid on proliferation of human gastric cancer SGC-7901 cells |
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| CHEN Ya-nan,SHAO Shu-li,HE Meng-qi,HUANG Xin,ZHANG Wei-wei,ZHANG Zhen-zhu.Effect of betulinic acid on proliferation of human gastric cancer SGC-7901 cells[J].Chinese Journal of Applied Physiology,2020,36(6):628-632. |
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| Authors: | CHEN Ya-nan SHAO Shu-li HE Meng-qi HUANG Xin ZHANG Wei-wei ZHANG Zhen-zhu |
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| Affiliation: | 1. College of Life Science and Agriculture and Forestry, Qiqihar University, Qiqihaer 161000;2. Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas, Qiqihar University, Qiqihaer 161000, China |
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| Abstract: | Objective: Human gastric cancer SGC-7901 cells were treated with betulinic acid(BA)at the concentrations of 0, 10, 20, and 30 μg/ml, and treated with conventional chemotherapeutic drug 5-Fu as a positive control to explore its effect on cell proliferation. Trypan blue and GIEMSA staining method were used to investigate the effect of BA on cell growth inhibition and clone formation. EdU method and flow cytometry were used to explore the proliferation and cell cycle of SGC-7901 cells after treated with BA, respectively. qRT-PCR and Western blot were also applied to determine the mRNA and protein levels of cyclin D1 and cyclin B1. Results: The cell growth inhibition rate was increased after treated with different concentrations of BA in SGC-7901 cells(P＜0.05). After treated for 48 h, BA decreased the clone information and cell proliferation of SGC-7901 cells markedly in dose-and time-dependent manners (P＜0.01). Flow cytometry analysis showed that BA obviously increased the proportion of SGC-7901 cells in G1 phase but decreased the proportion of those in S phase. qRT-PCR and Western blot analysis showed that the mRNA and protein levels of cyclin D1 and cyclin B1 were significantly downregulated by BA at different concentrations(P＜0.01). Compared with the 5-Fu control group, when the concentration of BA was 20 μg/ml and 30 μg/ml, the cell proliferation ability was significantly decreased, the cell cycle was inhibited, and the expression of cyclin was reduced (all P＜0.05). Conclusion: The betulinic acid regulates the proliferation of SGC-7901 cells by inhibiting the expressions of cyclin D1 and cyclin B1, which leads to cell cycle arrest and proliferative inhibition. |
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| Keywords: | betulinic acid SGC-7901 cells cell proliferation cell cycle |
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