| 苦参素对脂多糖诱导的海马神经元凋亡的保护作用 |
| |
| 引用本文： | 蔡艳,糜大国,蒋伟,周爱玲,姜晓燕,朱可,曹刚. 苦参素对脂多糖诱导的海马神经元凋亡的保护作用[J]. 中华全科医学, 2020, 18(10): 1660. DOI: 10.16766/j.cnki.issn.1674-4152.001586 |
| |
| 作者姓名： | 蔡艳 糜大国 蒋伟 周爱玲 姜晓燕 朱可 曹刚 |
| |
| 作者单位： | 1. 南通市中医院药剂科, 江苏 南通 226001; |
| |
| 基金项目： | 江苏省南通市卫生和计划生育委员会科研项目(WKZL2018037)江苏省“333工程”科研项目(BRA2018223) |
| |
| 摘 要： | 目的 探讨苦参素(OMT)对脂多糖(LPS)诱导的海马神经元凋亡的影响及其可能机制。 方法 根据海马神经元乳酸脱氢酶(LDH)释放率选择OMT的浓度进行实验分组。分为7组:正常对照组;脂多糖组;阿司匹林+脂多糖组;苦参素组;苦参素低剂量+脂多糖组;苦参素中剂量+脂多糖组;苦参素高剂量+脂多糖组。流式细胞仪检测细胞凋亡率;免疫荧光观察NF-κB/P65核易位情况;酶联免疫吸附(ELISA)技术测定海马神经元培养上清中炎症因子肿瘤坏死因子-α(TNF-α)和白细胞介素-1β(IL-1β)的水平。 结果 LPS组海马神经元凋亡率显著高于正常对照组(P<0.01),OMT预处理可以显著减少LPS刺激引起的海马神经元凋亡率(P<0.05)。LPS可以促进海马神经元NF-κB/P65由胞浆转位到胞核,提高海马神经元培养上清中TNF-α和IL-1β水平;而OMT预处理能显著减少LPS对海马神经元NF-κB/P65核易位作用及降低TNF-α和IL-1β的水平(P<0.05)。 结论 LPS诱导海马神经元NF-κB/P65核易位,导致炎症因子TNF-α和IL-1β的含量增加;OMT预处理可以抑制海马神经元NF-κB/P65核易位,减少TNF-α和IL-1β的分泌,从而抑制海马神经元的凋亡。
|
| 关 键 词： | 苦参素 海马神经元 细胞凋亡 炎症因子 |
| 收稿时间： | 2019-12-17 |
Protective effects of oxymatrine on LPS-induced neuronal apoptosis of hippocampal neurons |
| |
| Affiliation: | Department of Pharmacy, Nantong Hospial of Traditional Chinese Medicine, Nantong, Jiangsuu 226001, China |
| |
| Abstract: | Objective to investigate the effect and mechanisms of oxymatrine(OMT) on lipopolysaccharide(LPS) induced apoptosis of hippocampal neurons. Methods According to the release rate of lactate dehydrogenase(LDH) in hippocampal neurons, the concentration of OMT was selected for experimental grouping. There were seven groups:normal group, LPS group, Aspirin+LPS group, OMT group, OMT low-dose+LPS group, OMT mid-dose+LPS group and OMT high-dose+LPS group. Apoptosis rate was detected by flow cytometry. The nuclear translocation of NF-κB/P65 was observed by immunofluorescence. The level of tumor necrosis factor-α(TNF-α) and interleukin-1β(IL-1β) in culture supernatants of hippocampal neurons were determined by Enzyme-linked immunosorbent assay(ELISA). Results The apoptosis rate of hippocampal neurons in the LPS group was higher than normal group(P<0.01), and OMT could significantly reduce LPS stimulated apoptosis rate of hippocampal neurons(P<0.05). LPS promoted NF-κB/P65 shifted from cytoplasm to the nucleus, and increased the level of TNF-α and IL-1β in the culture supernatant of hippocampal neuron, while OMT reduced the LPS on hippocampal neurons NF-κB/P65 nuclear translocation and reduce the level of TNF-α and IL-1β. Conclusion LPS could induce NF-κB/P65 nuclear translocation in hippocampal neurons and result in increased levels of inflammatory factors TNF-α and IL-1β. OMT could weaken the nuclear translocation of NF-κB/P65 induced by LPS and reduce the level of TNF-α and IL-1β, which in turn to inhibit apoptosis of hippocampal neurons. |
| |
| Keywords: | |
|
| 点击此处可从《中华全科医学》浏览原始摘要信息 |
|
点击此处可从《中华全科医学》下载免费的PDF全文 |
|
