| 国产弹性蛋白酶浸泡法构建兔腹主动脉瘤及其随访 |
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| 引用本文： | 毕永华,伊梦飞,陈红梅,韩新巍,任建庄,尉泽鹏.国产弹性蛋白酶浸泡法构建兔腹主动脉瘤及其随访[J].介入放射学杂志,2020,29(4):376-379. |
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| 作者姓名： | 毕永华 伊梦飞 陈红梅 韩新巍 任建庄 尉泽鹏 |
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| 作者单位： | 450052 郑州大学第一附属医院放射介入科;郑州大学附属郑州中心医院超声科 |
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| 摘 要： | 目的采用国产猪胰弹性蛋白酶溶液主动脉周浸泡法构建兔腹主动脉瘤(AAA)模型,并随访其长期稳定性。方法 24只新西兰兔随机分成两组,实验组(n=12)用10μL浓度为10 U/μL国产猪胰弹性蛋白酶溶液浸润主动脉近分叉处血管段30 min,对照组用10μL 0.9%氯化钠溶液浸润30 min。术前和术后5、15、40、100、150 d分别经耳缘静脉造影测量主动脉内径。术后5、15、150 d造影后每组分别处死4只兔作苏木精-伊红(HE)、弹力纤维EVG染色和免疫组化染色。结果实验组术后5 d均形成AAA,主动脉直径100 d内基本稳定,150 d时明显缩小;组织学上术后5 d血管壁严重破坏、结构紊乱,可见红细胞渗出和炎性细胞浸润,弹力纤维和平滑肌细胞明显减少甚至消失,15 d时血管结构规整,可见部分残留弹力纤维,未见炎性细胞,150 d时管腔变窄,内膜过度增生,可见大量平滑肌细胞增生和紊乱的新生弹力纤维。对照组均未见AAA形成,病理学无明显变化。结论国产猪胰弹性蛋白酶溶液浸泡法可诱导兔AAA形成,操作简单、安全、有效。该模型有自愈倾向,但100 d内基本稳定,有助于AAA机制研究。
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| 关 键 词： | 国产猪胰弹性蛋白酶 兔 腹主动脉瘤 实验模型 基质金属蛋白酶 |
Establishment of abdominal aortic aneurysm induced by domestic elastase in experimental rabbit and follow-up observation |
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| BI Yonghua,YI Mengfei,CHEN Hongmei,HAN Xinwei,REN Jianzhuang,WEI Zepeng..Establishment of abdominal aortic aneurysm induced by domestic elastase in experimental rabbit and follow-up observation[J].Journal of Interventional Radiology,2020,29(4):376-379. |
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| Authors: | BI Yonghua YI Mengfei CHEN Hongmei HAN Xinwei REN Jianzhuang WEI Zepeng |
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| Affiliation: | Department of Interventional Radiology, First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province 450052, China |
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| Abstract: | Objective To establish a rabbit model of abdominal aortic aneurysm(AAA) by using periaortic immersion method with domestic porcine pancreatic elastase solution, and to observe its longterm stability. Methods Twenty-four New Zealand rabbits were randomly and equally divided into study group(n=12) and control group(n=12). For the rabbits of the study group the vascular segment near aortic bifurcation was immersed in 10 U/μL domestic porcine pancreatic elastase solution for 30 minutes, while for the rabbits of the control group the vascular segment near aortic bifurcation was immersed in 10 U/μL of saline for 30 minutes. The aortic diameter of immersed segment was measured by angiography through auriculus vein injection of contrast, which was carried out before treatment as well as at 5, 15, 40, 100 and 150 days after treatment. Every 4 rabbits from each group were sacrificed each time at postoperative 5, 15, and 150 days after angiography, and hematoxylin-eosin(HE) staining, elastic fibre EVG staining and immunohistochemical staining were made for laboratory examination. Results In the study group, the formation of AAA was demonstrated in all experimental rabbits five days after treatment, and the aortic diameter became basically stable within 100 days, which was significantly reduced in 150 days. Histologically, serious destruction of vascular wall with structural disorder, erythrocyte exudation, inflammatory cell infiltration, and significant reduction or even disappearance of elastic fibers and smooth muscle cells were observed in 5 days after treatment. At 15 days after treatment, the vascular structure was regularity, some residual elastic fibers with no inflammatory cells were demonstrated. At 150 days after treatment, the lumen became narrowed, the intima was remarkably hypertrophied, and the proliferation of large amount of smooth muscle cells and the disordered new elastic fibers were seen. In the control group, neither AAA formation nor obvious pathological changes were observed. Conclusion The periaortic immersion method with domestic porcine pancreatic elastase solution can reliably induce the formation of AAA in experimental rabbits. The method is simple, safe and effective. This AAA model has the tendency of self-healing, but it is basically stable within 100 days. This model can be reliably used in the study of the mechanism of AAA. |
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| Keywords: | domestic porcine elastase rabbit abdominal aortic aneurysm experimental model matrix metalloproteinase |
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