| miR-218-5p靶向下调LPAR3抑制宫颈癌细胞增殖、迁移和侵袭 |
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| 引用本文： | 王亚莉,周晓莉,刘 杰,赵嫦娥,汪黎明.miR-218-5p靶向下调LPAR3抑制宫颈癌细胞增殖、迁移和侵袭[J].现代肿瘤医学,2020,0(9):1446-1451. |
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| 作者姓名： | 王亚莉 周晓莉 刘 杰 赵嫦娥 汪黎明 |
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| 作者单位： | 1.中国人民解放军中部战区总医院妇产科，湖北 武汉 430070；2.湖北省妇幼保健院妇科，湖北 武汉 430070 |
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| 基金项目： | 湖北省自然科学基金项目(编号:WJ2015MA017) |
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| 摘 要： | 目的:探讨miR-218-5p通过调控LPAR3表达对宫颈癌细胞增殖、迁移和侵袭的影响。方法:qRT-PCR和Western blot检测人正常宫颈细胞H8和4种宫颈癌细胞（SiHa、HeLa、MS751和HT-3）中miR-218-5p和LPAR3的表达。以HeLa细胞为后续研究对象，分别构建过表达miR-218-5p和敲减LPAR3的HeLa细胞株，CCK-8法检测细胞存活情况，Transwell实验检测细胞的迁移和侵袭能力，Western blot检测细胞增殖相关蛋白CyclinD1、迁移侵袭相关蛋白MMP2和MMP9的表达。双荧光素酶报告基因实验和Western blot验证miR-218-5p和LPAR3的靶向调控关系。结果:与人正常宫颈细胞H8相比，4种宫颈癌细胞miR-218-5p的表达显著下调，LPAR3的表达显著上调。过表达miR-218-5p或敲减LPAR3均可抑制HeLa细胞的增殖、迁移和侵袭能力，抑制CyclinD1、MMP2和MMP9蛋白的表达。LPAR3是miR-218-5p的靶基因，miR-218-5p可负性调控LPAR3的表达。过表达LPAR3可逆转miR-218-5p对HeLa细胞增殖、迁移和侵袭的影响。结论:miR-218-5p通过靶向下调LPAR3表达抑制宫颈癌细胞的增殖、迁移和侵袭，miR-218-5p/LPAR3分子轴有望成为宫颈癌的潜在治疗靶点。
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| 关 键 词： | miR-218-5p LPAR3 宫颈癌 细胞增殖 迁移 侵袭 |
miR-218-5p inhibits the proliferation，migration and invasion of cervical cancer cells by down-regulating LPAR3 |
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| Wang Yali,Zhou Xiaoli,Liu Jie,Zhao Chang'e,Wang Liming.miR-218-5p inhibits the proliferation，migration and invasion of cervical cancer cells by down-regulating LPAR3[J].Journal of Modern Oncology,2020,0(9):1446-1451. |
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| Authors: | Wang Yali Zhou Xiaoli Liu Jie Zhao Chang'e Wang Liming |
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| Affiliation: | 1.Department of Obstetrics and Gynecology，General Hospital of the Central War Zone of the People's Liberation Army，Hubei Wuhan 430070，China；2.Department of Gynecology，Hubei Maternal and Child Health Hospital，Hubei Wuhan 430070，China. |
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| Abstract: | Objective:To investigate the effect of miR-218-5p on the proliferation，migration and invasion of cervical cancer cells by regulating the expression of LPAR3.Methods:qRT-PCR and Western blot were used to detect the expression of miR-218-5p and LPAR3 in human normal cervical cells H8 and 4 cervical cancer cells (SiHa，HeLa，MS751 and HT-3).HeLa cells were selected for follow-up studies.HeLa cell lines with over-expressing miR-218-5p and knocking LPAR3 were constructed，respectively.CCK-8 method was used to detect cell viability.Transwell assay was used to detect cell migration and invasion，and Western blot was used to detect the expression level of proliferation-related protein CyclinD1，migration-invasion related proteins MMP2 and MMP9.The dual luciferase reporter gene assay and Western blot were used to verify the targeted regulation between miR-218-5p and LPAR3.Results:Compared with human normal cervical cells H8，the expression of miR-218-5p was significantly down-regulated in four cervical cancer cells，and the expression of LPAR3 was significantly up-regulated.The over-expression of miR-218-5p or the knockdown of LPAR3 inhibited the proliferation，migration and invasion of HeLa cells，and inhibited the expression of CyclinD1，MMP2 and MMP9 proteins.LPAR3 was a target gene of miR-218-5p，and miR-218-5p negatively regulated the expression of LPAR3.The over-expression of LPAR3 reversed the effect of miR-218-5p on the proliferation，migration and invasion of HeLa cells.Conclusion:miR-218-5p inhibits the proliferation，migration and invasion of cervical cancer cells by down-regulating LPAR3.The miR-218-5p/LPAR3 molecular axis is expected to be a potential therapeutic target for cervical cancer. |
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| Keywords: | miR-218-5p LPAR3 cervical cancer cell proliferation migration invasion |
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