| KIF20A对胶质瘤细胞增殖、迁移和侵袭、凋亡及细胞周期分布的影响 |
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| 引用本文： | 刘纪营,吴妍妍,赵 杰,张五松,靳振伟,陈健辉,郭新宾.KIF20A对胶质瘤细胞增殖、迁移和侵袭、凋亡及细胞周期分布的影响[J].现代肿瘤医学,2020,0(17):2943-2947. |
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| 作者姓名： | 刘纪营 吴妍妍 赵 杰 张五松 靳振伟 陈健辉 郭新宾 |
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| 作者单位： | 1.项城市中医院介入科;2.脑病科;3.神经内科，河南 项城 466200;4.郑州大学第一附属医院神经介入科，河南 郑州 450000 |
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| 基金项目： | 河南省高等学校重点科研项目（编号:19A320034） |
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| 摘 要： | 目的:研究KIF20A对胶质瘤U87细胞系增殖、侵袭、迁移、凋亡及细胞周期分布的影响。方法:利用 RNA干扰技术下调胶质瘤细胞系 U87中 KIF20A 的表达。RT-qPCR 和 Western blot 分别检测 KIF20A 在mRNA 和蛋白水平上的表达。CCK-8 实验检测 U87细胞增殖能力的变化，Transwell 实验检测U87细胞迁移及侵袭能力的变化，流式细胞术检测 U87细胞凋亡及细胞周期的变化。结果:RT-qPCR和Western blot 结果显示 siRNA-KIF20A显著下调 KIF20A 的表达，CCK-8和Transwell 实验显示下调 KIF20A 的表达显著抑制了U87细胞的增殖、迁移及侵袭能力，流式细胞术结果显示下调 KIF20A 的表达显著促进了U87细胞的凋亡，诱导细胞周期阻滞在G0/G1期。结论:下调KIF20A的表达抑制胶质母细胞瘤细胞的增殖、迁移及侵袭，促进胶质母细胞瘤细胞的凋亡并诱导细胞周期G0/G1期阻滞。
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| 关 键 词： | 胶质瘤 KIF20A siRNA 生物学功能 |
Effects of KIF20A on the proliferation，migration and invasion，apoptosis and cell cycle distribution of glioma cells |
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| Liu Jiying,Wu Yanyan,Zhao Jie,Zhang Wusong,Jin Zhenwei,Chen Jianhui,Guo Xinbin.Effects of KIF20A on the proliferation，migration and invasion，apoptosis and cell cycle distribution of glioma cells[J].Journal of Modern Oncology,2020,0(17):2943-2947. |
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| Authors: | Liu Jiying Wu Yanyan Zhao Jie Zhang Wusong Jin Zhenwei Chen Jianhui Guo Xinbin |
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| Affiliation: | 1.Intervention Section；2.Department of Encephalopathy；3.Department of Neurology，Xiangcheng Traditional Chinese Medicine Hospital,Henan Xiangcheng 466200,China;4.Department of Neurointervention，the First Affiliated Hospital of Zhengzhou University，Henan Zhengzhou 450000，China. |
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| Abstract: | Objective:To study the effect of KIF20A on proliferation，invasion，migration，apoptosis and cell cycle distribution of human glioma cell line U87.Methods:RNA interference technique was used to down-regulate the expression of KIF20A in glioma cell line U87.RT-qPCR and Western blot were used to detect the expression of KIF20A at mRNA and protein levels，respectively.The change of proliferation ability of U87 cells was detected by CCK-8 experiment.The change of migration and invasion ability of U87 cells was detected by Transwell experiment，and the change of apoptosis and cell cycle of U87 cells was detected by flow cytometry.Results:The results of RT-qPCR and Western blot showed that siRNA-KIF20A significantly reduced the expression of KIF20A.The CCK-8 and Transwell experiments showed that down-regulation of KIF20A expression significantly inhibited the proliferation，migration and invasion of U87 cells.Flow cytometry showed that down-regulation of KIF20A expression significantly promoted apoptosis of U87 cells and induced cell cycle arrest in G0/G1 phase.Conclusion:Down-regulation of KIF20A expression inhibits proliferation，migration and invasion of glioblastoma cells，promotes apoptosis of glioblastoma cells and induces cell cycle G0/G1 phase arrest. |
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| Keywords: | glioma KIF20A siRNA biological function |
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