| 下调ALK2对人乳腺癌MDA-MB-468细胞增殖、侵袭和迁移能力的影响 |
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| 引用本文： | 费 嫦,王 林,陈湘莲,米 华,陈玲群,桂 芳.下调ALK2对人乳腺癌MDA-MB-468细胞增殖、侵袭和迁移能力的影响[J].现代肿瘤医学,2020,0(13):2194-2198. |
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| 作者姓名： | 费 嫦 王 林 陈湘莲 米 华 陈玲群 桂 芳 |
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| 作者单位： | 湖南医药学院检验医学院，湖南 怀化 418000 |
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| 基金项目： | 湖南省教育厅科研项目（编号:14C0908）；湖南医药学院科研项目（编号:2015008） |
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| 摘 要： | 目的:通过表达siALK2的重组腺病毒Ad-siALK2，下调人乳腺癌MDA-MB-468细胞ALK2的表达，研究其对MDA-MB-468细胞增殖、侵袭和迁移能力的影响。方法:以重组腺病毒Ad-siALK2感染MDA-MB-468细胞为实验组，空载病毒（RFP）作为感染对照，并设空白对照组，通过倒置荧光显微镜观察和RT-PCR检测病毒在MDA-MB-468细胞中的表达水平，通过MTT、平板集落形成、细胞划痕和Transwell侵袭实验检测细胞的增殖、集落形成、迁移及侵袭能力。结果:倒置荧光显微镜观察显示Ad-siALK2感染MDA-MB-468细胞的荧光效率高；RT-PCR证实Ad-siALK2感染的MDA-MB-468细胞ALK2的mRNA表达显著下降；MTT法、平板集落形成实验、细胞划痕实验及Transwell侵袭实验证实MDA-MB-468细胞Ad-siALK2组相比对照组细胞的增殖活力、集落形成率及划痕愈合率均显著降低（P<0.05），穿膜细胞数也明显减少（P<0.05）。结论:下调ALK2表达后可以在体外抑制人乳腺癌MDA-MB-468细胞的增殖、迁移与侵袭，为后续实验研究奠定了基础。
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| 关 键 词： | 乳腺肿瘤 细胞增殖 ALK2 |
Effect of down-regulated ALK2 on proliferation，migration and invasion abilities of human breast cancer MDA-MB-468 cells |
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| Fei Chang,Wang Lin,Chen Xianglian,Mi Hua,Chen Lingqun,Gui Fang.Effect of down-regulated ALK2 on proliferation，migration and invasion abilities of human breast cancer MDA-MB-468 cells[J].Journal of Modern Oncology,2020,0(13):2194-2198. |
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| Authors: | Fei Chang Wang Lin Chen Xianglian Mi Hua Chen Lingqun Gui Fang |
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| Affiliation: | College of Laboratorial，Hunan University of Medicine，Hunan Huaihua 418000，China. |
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| Abstract: | Objective:To study the effect of siALK2 expressed by recombinant adenovirus Ad-siALK2 through RNA interference(siRNA) on the proliferation，migration and invasion abilities of human breast cancer MDA-MB-468 cell line.Methods:MDA-MB-468 cells were infected with adenovirus siALK2 and RFP respectively.The recombinant adenovirus was identificated by RT-PCR and the detection of fluorescence infection efficiency of cells by inverted fluorescence microscop.The proliferation，migration and invasion of MDA-MB-468 cells were estimated by MTT assay，colony-forming assay，wounding healing assay and Transwell assay.Results:Inverted fluorescence microscope showed that Ad-siALK2 had high fluorescence efficiency in MDA-MB-468 cells.ALK2 mRNA expression in MDA-MB-468 cells was significantly decreased by RT-PCR.The proliferation，migration and invasion of MDA-MB-468/siALK2 cells were estimated by MTT assay，colony-forming assay，wounding healing assay and Transwell assay showed that the proliferation activity，colony formation rate，wound healing rate and count of cells crossing the matrix barrier were significantly reduced in MDA-MB-468/siALK2 group than control group（P<0.05），ALK2 expression was remarkably decreased in MDA-MB-468/siALK2 cells（P<0.05）．Conclusion:These results demonstrated that down-regulating ALK2 expression can inhibited the proliferation，migration and invasion of MDA-MB-468 cells in vitro and laid the foundation for the follow-up study of mechanisms. |
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| Keywords: | breast tumor cell proliferation ALK2 |
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