| 高通量测序解析烟草苗床期基质细菌和真菌群落结构与多样性变化 |
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| 引用本文： | 罗飞,李文红,刘亭亭,汪汉成,孙美丽,蔡刘体,穆青,余知和.高通量测序解析烟草苗床期基质细菌和真菌群落结构与多样性变化[J].中国烟草学报,2023,29(2):79-88. |
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| 作者姓名： | 罗飞 李文红 刘亭亭 汪汉成 孙美丽 蔡刘体 穆青 余知和 |
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| 作者单位： | 1.长江大学生命科学学院, 湖北荆州 434025 |
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| 基金项目： | 国家自然科学基金项目31960550贵州省烟草公司黔西南州公司科技项目州烟技(2021)4号2021-06中国烟草总公司贵州省公司科技项目201914中国烟草总公司贵州省公司科技项目2020XM03 |
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| 摘 要： | 【背景和目的】烟苗的生长发育状况与育苗基质微生物密切相关，为了解烟苗育苗阶段基质细菌和真菌群落结构与多样性特征。【方法】采用IlluminaMiSeq高通量测序对育苗基质中细菌和真菌群落进行测序与分析。【结果】不同育苗阶段基质（播种后第10～66 d）真菌和细菌在菌群结构及相对丰度均存在差异。基质中优势细菌门均为变形菌门Proteobacteria(65.60%～72.80%)、拟杆菌门Bacteroidetes(16.75%～21.35%)和酸杆菌门Acidobacteria(1.38%～4.67%)；优势细菌属均为德沃斯氏菌属Devosia(4.81%～14.20%)、罗河杆菌属Rhodanobacter(1.60%～6.19%)、戴氏菌属Dyella(0.38%～8.92%)等，其中，德沃斯氏菌属和罗河杆菌属在播种第66 d的相对丰度较第10 d分别降低8.6%和3.31%。播种第38 d基质细菌Shannon多样性指数和ACE丰富度指数最低，分别为7.25和1161.10；播种第24 d和66 d基质细菌Shannon多样性指数最高，分别为8.15和8.01。基质中优势真菌门均...
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| 关 键 词： | 苗床期 育苗基质 Illumina MiSeq高通量测序 群落结构 烟苗 微生物多样性 |
| 收稿时间： | 2022-03-20 |
Community structure and diversity of bacteria and fungi in substrate of tobacco seedling based on high-throughput sequencing |
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| Affiliation: | 1.College of College of Life Science, Yangtze University, Jingzhou, Hubei 434025, China2.Guizhou Institute of Plant Protection, Guizhou Academy of Agricultural Sciences, Guiyang 550006, China3.Guizhou Academy of Tobacco Science, Guiyang, Guizhou 550081, China4.Qianxinan Municipal Company of Guizhou Tobacco Company, Xingyi, Guizhou, 562400, China |
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| Abstract: | Background and Objective The growth, development and health status of tobacco seedlings are closely related to substrate microorganisms. This study aims to understand the community structure and diversity characteristics of bacteria and fungi in substrate of tobacco seedlings at seedling stages. Methods Illumina MiSeq sequencing was used to sequence and analyze the bacterial and fungal communities in substrate of tobacco seedlings. Results The structure and relative abundance of fungi and bacteria in substrate were different at the seedling stages (Days 10 to 66 after seeding). The dominant bacteriophyta in the substrate were Proteobacteria (65.60%-72.80%), Bacteroidetes (16.75%-21.35%) and Acidobacteria (1.38%-4.67%). The dominant bacteria genera were Devosia (4.81%-14.20%), Rhodanobacter (1.60%-6.19%) and Dyella (0.38%-8.92%), etc. Among them, the relative abundance of Devosia and Rhodobacter in the 66th day of sowing decreased by 8.6% and 3.31% as compared with that at the 10th day, respectively. At the 38th day of sowing, the Shannon diversity index and ACE richness index of bacteria in substrate were 7.25 and 1161.10. On the other hand, the highest Shannon diversity index was 8.15 and 8.01 at the 24th and 66th day of sowing, respectively. The dominant Eumycophyta were Ascomycota (16.84%-23.55%) and Basidiomycota (4.93%-11.95%) and Aphelidiomycota (0.04%-5.32%). The dominant fungi genera were Pseudogymnoascus (0.42%-11.63%), Arthrobotrys (0.28%-14.29%) and Tomentella (0.21%-8.96%), etc. Among them the relative abundance of Arthrobotrys and Tomentella at the 52th day of sowing increased by 14.01% and 8.57% as compared with that at the 10th day, respectively. The lowest diversity index of fungi in substrate was 3.93 at the 24th day of sowing. The highest Shannon diversity index was 5.57 and 5.86 at the 10th and 52nd days of sowing, respectively. The functional prediction showed that the main functions of bacteria in substrate were metabolic genetic information processing and environmental information processing within 66 days of sowing. Fungi were mainly saprophytic and ectomycorrhizal Fungi. Conclusion The results showed that there were no significant difference in the community structure of bacterial and fungi in substrate during the tobacco seedling stages, but their relative abundance and diversity index were different at different seedling stages. |
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