Serological Diagnosis of Tropical Canine Pancytopenia by Indirect Immunofluorescence |
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| Authors: | Miodrag Ristic David L Huxsoll Rita M Weisiger Paul K Hildebrandt and M B A Nyindo |
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| Affiliation: | .Department of Veterinary Pathology and Hygiene, College of Veterinary Medicine, University of Illinois at Champaign-Urbana, Urbana, Illinois 61801;.Division of Veterinary Medicine, Walter Reed Army Institute of Research, Walter Reed Army Medical Center, Washington, D.C. 20012 |
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| Abstract: | An indirect fluorescent-antibody test for detection and titration of antibodies to Ehrlichia canis, the causative agent of tropical canine pancytopenia, has been described. The organism propagated by an in vitro technique in canine blood monocytes served as an antigen in the test. The specificity of the test was revealed by absence of cross-reactivity between the antigen and sera from dogs infected with various common pathogens and specific sera against eight rickettsial species. The accuracy of the test was ascertained by isolation of the organism from reactor dogs located in and outside the United States. Histopathological examination of nine reactor dogs revealed plasmacytosis of meninges and kidneys in eight of them. |
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