| LncRNA FENDRR调控miR-942-5p表达对食管癌细胞增殖、上皮间充质转化及侵袭的影响 |
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| 引用本文： | 杨海龙,王丁丁,陈雨桐,陈晓伟,李 冰,王欣丽.LncRNA FENDRR调控miR-942-5p表达对食管癌细胞增殖、上皮间充质转化及侵袭的影响[J].现代肿瘤医学,2023,0(13):2425-2430. |
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| 作者姓名： | 杨海龙 王丁丁 陈雨桐 陈晓伟 李 冰 王欣丽 |
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| 作者单位： | 1.哈励逊国际和平医院胸外科;2.神经内一科，河北 衡水 053000 |
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| 基金项目： | 河北省衡水市重点研发计划项目（编号:2021014030Z） |
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| 摘 要： | 目的:探究LncRNA FENDRR通过miR-942-5p对EC细胞增殖、上皮间充质转化（epithelial-mesenchymal transition，EMT）和侵袭的影响。方法:收集EC和食管正常组织，将EC-113细胞分为BC组（不转染）、过表达（OE）-FENDRR-NC组（转染pcDNA空质粒）、OE-FENDRR组（转染pcDNA-LncRNA FENDRR质粒）、OE-FENDRR+miR-942-5p mimics-NC组（共转染pcDNA-LncRNA FENDRR+miR-942-5p mimics-NC）和OE-FENDRR+miR-942-5p mimics组（共转染pcDNA-LncRNA FENDRR+miR-942-5p mimics）。实时荧光定量聚合酶链反应（real-time fluorescence quantitative polymerase chain reaction,RT-qPCR）分析组织或细胞中LncRNA FENDRR、miR-942-5p表达量；细胞计数试剂盒-8（cell counting kit-8，CCK-8）法、裸鼠成瘤实验、Transwell法分析EC-113细胞体外和体内增殖和侵袭活性；双荧光素酶报告实验和RNA pull down实验分析LncRNA FENDRR和miR-942-5p的靶向关系；蛋白质印迹法（Western blot）分析EMT相关蛋白表达。结果:转染pcDNA-LncRNA FENDRR质粒可增高EC-113细胞的LncRNA FENDRR表达量，降低miR-942-5p表达，同时降低体外和体内增殖活性、侵袭数量、N-钙黏附素（N-cadherin，N-CAD）、波形蛋白（vimentin，VIM）和Snial及基质金属蛋白酶9（matrix metalloproteinase 9，MMP9）蛋白水平，增加E-钙黏附素（E-cadherin，E-CAD）蛋白水平（P＜0.05），而miR-942-5p mimics逆转了上述作用。LncRNA FENDRR可靶向并下调miR-942-5p表达。结论:LncRNA FENDRR为miR-942-5p分子海绵，可以下调miR-942-5p表达，从而抑制EC细胞增殖、EMT和侵袭能力。
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| 关 键 词： | 长链非编码RNA FENDRR 微小RNA-199a-5p 食管癌 增殖 上皮间充质转化 侵袭 |
Influences of LncRNA FENDRR on the proliferation,epithelial-mesenchymal transition and invasion of esophageal cancer cells by regulating the expression of miR-942-5p |
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| YANG Hailong,WANG Dingding,CHEN Yutong,CHEN Xiaowei,LI Bing,WANG Xinli.Influences of LncRNA FENDRR on the proliferation,epithelial-mesenchymal transition and invasion of esophageal cancer cells by regulating the expression of miR-942-5p[J].Journal of Modern Oncology,2023,0(13):2425-2430. |
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| Authors: | YANG Hailong WANG Dingding CHEN Yutong CHEN Xiaowei LI Bing WANG Xinli |
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| Affiliation: | 1.Department of Thoracic Surgery;2.Department of Neurology,Harrison International Peace Hospital,Hebei Hengshui 053000,China. |
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| Abstract: | Objective:To investigate the effect of lncRNA FENDRR on EC cell proliferation,epithelial-mesenchymal transition(EMT) and invasion via miR-942-5p.Methods:EC and esophageal normal tissues were collected,and EC-113 cells were separated into BC group(without transfection),overexpression(OE)-FENDRR-NC group(transfected with pcDNA empty plasmid),OE-FENDRR group(transfected with pcDNA-LncRNA FENDRR plasmid),OE-FENDRR+miR-942-5p mimics-NC group(co-transfected with pcDNA-LncRNA FENDRR+miR-942-5p mimics-NC) and OE-FENDRR+miR-942-5p mimics group(co-transfection of pcDNA-LncRNA FENDRR+miR-942-5p mimics).Real-time fluorescence quantitative polymerase chain reaction(RT-qPCR) was performed to analyze LncRNA FENDRR and miR-942-5p expression in tissues or cells.Cell counting kit-8 (CCK-8) method,nude mouse tumorigenic experiment and Transwell method were performed to analyze the proliferation and invasion activities of EC-113 cells in vitro and in vivo.Dual-luciferase reporter assay and RNA pull down assay were performed to analyze the targeting relationship between LncRNA FENDRR and miR-942-5p.Western blot was performed to analyze EMT-related protein expression.Results:Transfection of pcDNA-LncRNA FENDRR plasmid was able to increase the expression of LncRNA FENDRR in EC-113 cells,decrease the expression of miR-942-5p,and decrease the proliferation activity and invasion number in vitro and in vivo,levels of N-cadherin(N-CAD),vimentin(VIM),snial and matrix metalloproteinase 9(MMP9) proteins,and increase the E-cadherin(E-CAD) protein level(P＜0.05),while miR-942-5p mimics reversed the above effects.LncRNA FENDRR was able to target and downregulate miR-942-5p expression.Conclusion:LncRNA FENDRR is a miR-942-5p molecular sponge,which can down-regulate the expression of miR-942-5p,thereby inhibiting proliferation,EMT and invasion behavior of EC cells. |
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| Keywords: | long non-coding RNA FENDRR microRNA-199a-5p esophageal cancer proliferation epithelial-mesenchymal transition invasion |
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