| 电子供体刺激下厌氧微生物对1, 2-二氯乙烷的降解效果 |
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| 引用本文： | 孙仲平,吴乃瑾,魏文侠,宋云.电子供体刺激下厌氧微生物对1, 2-二氯乙烷的降解效果[J].环境科学研究,2018,31(8):1431-1438. |
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| 作者姓名： | 孙仲平 吴乃瑾 魏文侠 宋云 |
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| 作者单位： | 轻工业环境保护研究所, 工业场地污染与修复北京市重点实验室, 北京 100089 |
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| 基金项目： | 北京市科技计划项目（No.Z161100001216008）；北京市改革与发展专项（No.613-2017A-22）；中国博士后基金二等资助（No.2017M610806） |
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| 摘 要： | 通过添加电子供体进行原位生物刺激可有效降解地下水中的氯代烃，目前国内外常采用接种高效微生物的方法强化这一降解过程，鲜见仅利用实际污染场地含水层土著菌开展的生物降解试验.从北京市某氯代烃污染场地采集地下水样品，仅接种场地含水层沉积物，利用微宇宙实验体系，初步探讨了添加不同种类和质量浓度的电子供体对地下水中1，2-二氯乙烷的去除效果影响.结果表明:①同时添加初级生长基质和电子供体对反应体系进行生物刺激可显著降解地下水中的1，2-二氯乙烷.60 d后，添加1 g/L醋酸钠的反应体系中1，2-二氯乙烷的去除率最高，可达93.9%；添加1 g/L的乳酸钠反应体系次之，去除率为82.2%；添加1 g/L的乳酸的反应体系去除率最低，为61.8%.并且添加醋酸钠的试验体系可长时间维持中性pH及较低的氧化还原电位.②相同条件下，对同种电子供体来说，添加量为1 g/L的试验组中1，2-二氯乙烷的降解效果优于添加量为2 g/L的试验组，推测较高质量浓度的电子供体可对微生物的生命活动产生抑制.③对反应体系中间产物进行监测，仅监测到了乙烯的产生，表明在试验周期内无明显毒副产物积累，1，2-二氯乙烷的降解较为彻底.研究显示，醋酸钠为该试验体系中最适电子供体，添加适宜质量浓度醋酸钠对土著菌进行生物刺激可实现对1，2-二氯乙烷的绿色降解.
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| 关 键 词： | 生物修复 1 2-二氯乙烷 地下水 电子供体 |
| 收稿时间： | 2018/1/26 0:00:00 |
| 修稿时间： | 2018/5/4 0:00:00 |
Effect of Anaerobic Microbial Degradation of 1,2-Dichloroethane Stimulated by Electron Donor |
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| SUN Zhongping,WU Naijin,WEI Wenxia and SONG Yun.Effect of Anaerobic Microbial Degradation of 1,2-Dichloroethane Stimulated by Electron Donor[J].Research of Environmental Sciences,2018,31(8):1431-1438. |
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| Authors: | SUN Zhongping WU Naijin WEI Wenxia and SONG Yun |
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| Affiliation: | Beijing Key Laboratory of Industrial Land Contamination and Remediation, Environmental Protection Research Institute of Light Industry, Beijing 100089, China |
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| Abstract: | Effective degradation of chlorinated hydrocarbons in groundwater can be achieved by in-situ biostimulation via adding electron donors. At present, the method of inoculating highly effective microorganisms is often used to enhance the degradation process at home and abroad, and few tests were carried out only using indigenous bacteria. The present study collected groundwater samples from a chlorohydrocarbon contaminated site in Beijing and inoculated only the sediment in the aquifer of the site. The effects of different types and concentrations of electronic donors on the removal of 1,2-dichloroethane were preliminarily investigated by the microcosm experiments. The results showed that:(1) Adding primary substrate and electron donors during biological stimulation can promote the degradation of 1,2-dichloroethane significantly. After 60 days, the degradation rate of 1,2-dichloroethane were 93.9%, 82.2% and 61.8%, respectively, with sodium acetate and sodium lactate, lactic acid as electron donors. In addition, adding sodium acetate could maintain a neutral pH and a lower redox potential for a long time. (2) The 1,2-dichloroethane removal rate of the 1 g/L group was better than that of the 2 g/L electron donor group for the same electron donor, which suggesting that the addition of high concentration of electron donor could inhibit the activity of microorganism. (3) During the process, the intermediate products of the reaction system were monitored, and only ethylene was detected, which indicated that there was no obvious accumulation of by-products and 1,2-dichloroethane was thorough degraded. The results show that sodium acetate was the most suitable electron donor among the three electron donors chosen in the experiment, and green degradation of 1,2-dichloroethane could be achieved by native bacteria with the stimulation of appropriate concentration of sodium acetate. |
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| Keywords: | bioremediation 1 2-dichloroethane groundwater electron donor |
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