| 高脂环境下雄激素缺乏对小型猪内脏脂肪蓄积和炎症基因表达的影响 |
| |
| 引用本文： | 蔡兆伟,凌云,蔡月琴,朱科燕,陈诚.高脂环境下雄激素缺乏对小型猪内脏脂肪蓄积和炎症基因表达的影响[J].实验动物与比较医学,2017,25(1):74-78,84. |
| |
| 作者姓名： | 蔡兆伟 凌云 蔡月琴 朱科燕 陈诚 |
| |
| 作者单位： | 浙江中医药大学动物实验研究中心/比较医学研究中心, 杭州 310053;浙江中医药大学动物实验研究中心/比较医学研究中心, 杭州 310053;浙江中医药大学动物实验研究中心/比较医学研究中心, 杭州 310053;浙江中医药大学动物实验研究中心/比较医学研究中心, 杭州 310053;浙江中医药大学动物实验研究中心/比较医学研究中心, 杭州 310053 |
| |
| 基金项目： | 浙江省公益性技术应用研究（实验动物）计划项目（编号：2014C37010）；国家自然科学基金项目（编号：31200921）。 |
| |
| 摘 要： | 目的 探讨高脂环境下雄激素缺乏对小型猪内脏脂肪沉积、血清激素以及炎症相关基因表达的影响。方法 将性成熟的雄性五指山小型猪随机分成三组，即不去势组（SHAM），去势组（CAS）和去势加睾酮组（CAS+T），每组6只，所有动物均饲喂高脂饲料。12周实验结束时，采血检测血清激素水平变化，分离内脏脂肪并进行称重，采用荧光定量PCR技术检测脂肪合成与脂肪分解基因以及炎症相关基因的表达。结果 （1）去势显著减少高脂饮食小型猪血清睾酮含量但增加其血清瘦素含量，给予外源性睾酮处理能够恢复去势小型猪血清睾酮和瘦素水平；（2）去势导致高脂饮食小型猪内脏脂肪大量沉积，给予睾酮处理后，去势小型猪内脏脂肪含量显著降低；（3）去势和睾酮处理对高脂饮食小型猪内脏脂肪组织FAS、ACC、HSL和ATGL等脂肪代谢基因的表达没有显著影响；（4）去势显著上调高脂饮食小型猪内脏脂肪组织Leptin、CD68、CCL16、CCL23和SAA等炎症基因的表达，采用外源性睾酮处理去势小型猪则能降低上述基因的表达。结论 去势诱导的雄激素缺乏促进高脂饮食小型猪内脏脂肪沉积，并上调其内脏脂肪组织中炎症基因的表达，采用睾酮处理则能够改善去势小型猪内脏脂肪蓄积和炎症反应。
|
| 关 键 词： | 雄激素 小型猪 肥胖 脂肪沉积 炎症基因 |
| 收稿时间： | 2016/6/23 0:00:00 |
Effects of androgen deficiency on visceral fat accumulation and inflammatory gene expression in miniature pigs fed a high-fat diet |
| |
| CAI Zhao-wei,LING Yun,CAI Yue-qin,ZHU Ke-yan and CHEN Cheng.Effects of androgen deficiency on visceral fat accumulation and inflammatory gene expression in miniature pigs fed a high-fat diet[J].Laboratory Animal and Comparative Medicine,2017,25(1):74-78,84. |
| |
| Authors: | CAI Zhao-wei LING Yun CAI Yue-qin ZHU Ke-yan and CHEN Cheng |
| |
| Affiliation: | Laboratory Animal Research Center/Comparative Medical Research Center, Zhejiang Chinese Medical University, Hangzhou 310053, China;Laboratory Animal Research Center/Comparative Medical Research Center, Zhejiang Chinese Medical University, Hangzhou 310053, China;Laboratory Animal Research Center/Comparative Medical Research Center, Zhejiang Chinese Medical University, Hangzhou 310053, China;Laboratory Animal Research Center/Comparative Medical Research Center, Zhejiang Chinese Medical University, Hangzhou 310053, China;Laboratory Animal Research Center/Comparative Medical Research Center, Zhejiang Chinese Medical University, Hangzhou 310053, China |
| |
| Abstract: | Objective The aim of this study was to explore the effect of androgen deficiency on serum hormone levels, visceral fat accumulation and inflammatory gene expression in miniature pigs fed a high-fat diet (HFD). Methods Sexually mature male Chinese Wuzhishan miniature pigs were divided into three groups (animals/group) as follows:intact male pigs (SHAM), castrated male pigs (CAS) and castrated male pigs plus testosterone treatment (CAS+T). The pigs were fed a HFD diet for 12 weeks. Serum levels of testosterone and leptin were measured and visceral fat were dissected and weighted. qRT-PCR was performed to determine the mRNA expression levels of lipogenic, lipolysis and inflammation related genes. Results (1) Serum testosterone levels were significantly decreased but serum leptin levels were significantly increased in the castrated pigs. These effects were recovered after testosterone treatment. (2) Visceral fat percentage was significantly increased in the castrated pigs, and testosterone treatment reduced the increased visceral fat in the castrated pigs. (3) Castration and testosterone treatment had no significant effects on the expression levels of lipogenic genes (FAS and ACC) and lipolysis genes (HSL and ATGL) in pigs fed a HFD. (5) Castration significantly induced the expressions of inflammatory genes including Leptin, CD68, CCL16, CCL23 and SAA, and testosterone treatment recovered the expressions of the above genes in the castrated pigs. Conclusions Castration-induced testosterone deficiency promotes visceral fat accumulation and upregulates the expression levels of inflammatory genes in miniature pigs fed a HFD. Moreover, testosterone treatment ameliorates castration-induced visceral fat accumulation and inflammatory response in HFD-fed pigs. |
| |
| Keywords: | Testosterone Miniature pigs Obesity Fat accumulation Inflammatory gene |
|
| 点击此处可从《实验动物与比较医学》浏览原始摘要信息 |
|
点击此处可从《实验动物与比较医学》下载全文 |
|
