| 废弃烟叶中茄尼醇对照品的制备 |
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| 引用本文： | 舒希凯,林小静,王岱杰,刘代成,耿岩玲,刘静,王晓.废弃烟叶中茄尼醇对照品的制备[J].山东科学,2011,24(3):47-50. |
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| 作者姓名： | 舒希凯 林小静 王岱杰 刘代成 耿岩玲 刘静 王晓 |
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| 作者单位： | 1.山东师范大学生命科学学院，山东 济南 250014；
2.山东省科学院中药过程控制研究中心，山东省分析测试中心，山东 济南 250014 |
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| 基金项目： | 山东省科技攻关项目(2007GG20009005)；济南市高等院校自主创新计划(201004010)；济南市留学人员创业计划 |
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| 摘 要： | 建立从废弃烟叶中分离高纯度茄尼醇对照品的方法。首先采用石油醚萃取烟叶，动态皂化法皂化，硅胶柱层析进行粗分。制备型高效液相色谱采用甲醇∶乙醇=60∶40进行精制纯化。通过以上方法所得产物经ESI-MS、1H-NMR和13C-NMR鉴定结构，高效液相色谱法测得纯度为99.7%，满足对照品的要求。本方法简便快捷，重现性好，所得产物纯度高，适合茄尼醇对照品的制备。
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| 关 键 词： | 废弃烟叶 茄尼醇 制备 |
| 收稿时间： | 2011-03-04 |
Preparation of reference material of solanesol from waste tobacco leaves |
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| SHU Xi-kai,LIN Xiao-jing,WANG Dai-jie,LIU Dai-cheng,GENG Yan-ling,LIU Jing,WANG Xiao.Preparation of reference material of solanesol from waste tobacco leaves[J].Shandong Science,2011,24(3):47-50. |
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| Authors: | SHU Xi-kai LIN Xiao-jing WANG Dai-jie LIU Dai-cheng GENG Yan-ling LIU Jing WANG Xiao |
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| Affiliation: | 1.College of Life Science, Shandong Normal University, Jinan 250014, China;
2. Process Control Research Center of Traditional Chinese Medicine, Shandong Analysis and Test Center, Shandong Academy of Sciences, Jinan 250014, China |
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| Abstract: | This paper is to establish an approach to separate high-purity reference material of solanesol from tobacoo leaves.We initially obtained crude reference material of solanesol with petroleum ether extraction,dynamic saponification and silica gel chromatography.We then employed preparative high-performance liquid chromatography whose ingredient ratio was 60% methanol and 40% ethanol to refine and purify the acquired crude reference material of solanesol.The purified product whose structure was identified with ESI-MS,IR,1H-NMR and 13C-NMR and whose purity of 99.7% was determined with HPLC satisfied the requirement of reference material.Such an approach is simple,rapid and reproductive and its product has quite high purity so it is suitable for the preparation of reference material of solanesol. |
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| Keywords: | waste tobacoo leaves solanesol preparation |
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