| pan-RAF抑制剂LY3009120对分化型甲状腺癌细胞增殖、侵袭和迁移的影响及可能机制 |
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| 引用本文： | 樊新龙,郭 囡,高 鑫,杨 骁,石 岗,赵月皎.pan-RAF抑制剂LY3009120对分化型甲状腺癌细胞增殖、侵袭和迁移的影响及可能机制[J].现代肿瘤医学,2018,0(22):3554-3557. |
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| 作者姓名： | 樊新龙 郭 囡 高 鑫 杨 骁 石 岗 赵月皎 |
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| 作者单位： | 1.中国医科大学肿瘤医院，辽宁省肿瘤医院头颈外科；2.结直肠外科,辽宁 沈阳 110042 |
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| 基金项目： | 沈阳市科技计划项目（编号:17-230-9-52，18-014-4-75） |
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| 摘 要： | 目的:探讨pan-RAF抑制剂LY3009120对甲状腺癌细胞增殖、侵袭和迁移的影响及可能机制。方法:以不同浓度的LY3009120作用于MDA-T32细胞，应用CCK-8法检测细胞的增殖能力。以半数致死浓度（half maximal inhibitory concentration，IC50）作用于细胞，划痕实验检测细胞迁移能力，Transwell实验检测细胞侵袭能力。Western blotting法检测细胞中E-cadherin、N-cadherin和Snail的表达。结果:通过CCK-8法检测显示LY3009120能明显抑制MDA-T32细胞的增殖。划痕实验和Transwell实验结果显示LY3009120能抑制细胞的迁移和侵袭。Western blotting结果显示LY3009120促进MDA-T32细胞中E-cadherin的表达，抑制N-cadherin和Snail的表达。结论:LY3009120能够抑制甲状腺癌细胞系MDA-T32的增殖，可能通过上皮间质转换机制抑制细胞的侵袭和迁移。
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| 关 键 词： | LY3009120 甲状腺癌 增殖 侵袭 迁移 |
Effect of pan-RAF inhibitor LY3009120 on proliferation,invasion and metastasis of differentiated thyroid carcinoma and its possible mechanism |
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| Fan Xinlong,Guo Nan,Gao Xin,Yang Xiao,Shi Gang,Zhao Yuejiao.Effect of pan-RAF inhibitor LY3009120 on proliferation,invasion and metastasis of differentiated thyroid carcinoma and its possible mechanism[J].Journal of Modern Oncology,2018,0(22):3554-3557. |
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| Authors: | Fan Xinlong Guo Nan Gao Xin Yang Xiao Shi Gang Zhao Yuejiao |
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| Affiliation: | 1.Department of Head and Neck Surgery；2.Department of Colorectal Surgery,Cancer Hospital of China Medical University,Liaoning Cancer Hospital & Insititute,Liaoning Shenyang 110042,China. |
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| Abstract: | Objective:To investigate the effects of LY3009120 on the proliferation,invasion and metastasis of thyroid cancer cell lines and its possible mechanisms.Methods:MDA-T32 cells were treated with different concentrations of LY3009120 and cell proliferation was detected by CCK-8.The half maximal inhibitory concentration(IC50) was used to perform wound healing assay and Transwell assay to detect cell migration and invasion abilities.The expressions of E-cadherin,N-cadherin and Snail were detected by Western blotting.Results:LY3009120 suppressed the proliferation of MDA-T32 cells significantly.LY3009120 inhibited MDA-T32 cell invasion and migration.Moreover,LY3009120 increased the expression of E-cadherin but suppressed N-cadherin and Snail expressions.Conclusion:LY3009120 inhibited MDA-T32 cell proliferation,invasion and metastasis maybe through epithelial-mesenchymal transition mechanism. |
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| Keywords: | LY3009120 thyroid cancer proliferation invasion migration |
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