| 氢气水对大鼠碱烧伤角膜新生血管抑制作用的研究 |
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| 引用本文： | 温舒,杨炜.氢气水对大鼠碱烧伤角膜新生血管抑制作用的研究[J].眼科新进展,2018,0(1):039-43. |
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| 作者姓名： | 温舒 杨炜 |
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| 作者单位： | 832000 新疆维吾尔自治区石河子市，石河子大学医学院第一附属医院眼科 |
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| 摘 要： | 目的 研究氢气水（H2水）滴眼对大鼠碱烧伤诱导角膜新生血管（corneal neovascularization，CNV）的抑制作用并探讨H2水抑制CNV的机制。方法 SPF级SD大鼠48只48眼，成功建立碱烧伤诱导CNV模型，随机分为空白组、对照组和治疗组。治疗组给予1.6×10-6 H2水滴眼，对照组给予妥布霉素地塞米松眼液滴眼，空白组不做任何处理。分别在模型建立后第1天、第3天、第7天、第14天测量CNV长度，计算CNV面积。碱烧伤后第14天深麻醉下取大鼠角膜及房水，免疫组织化学染色法检测角膜组织中PEDF蛋白表达，RT-PCR法检测角膜组织中PEDF mRNA表达，ELISA检测房水内TNF-α蛋白表达。结果 角膜碱烧伤后第3天、第7天、第14天，治疗组新生血管面积分别为（3.26±1.82）mm2、（8.59±3.98）mm2和（3.24±2.34）mm2；对照组分别为（4.27±2.68）mm2、（6.44±4.67）mm2和（15.98±4.75）mm2；空白组分别为（9.49±2.79）mm2、（17.71±4.06）mm2和（25.35±1.40）mm2。碱烧伤后第3天、7天和14天，治疗组和对照组均小于空白组，差异均有显著统计学意义（均为P＜0.01）；碱烧伤后第3天和第7天，治疗组与对照组差异均无统计学意义（均为P>0.05）；碱烧伤后第14天治疗组明显小于对照组，差异具有显著统计学意义（P＜0.01）。碱烧伤后第14天，免疫组织化学染色结果显示，治疗组角膜上皮层PEDF蛋白表达较对照组和空白组明显增强，基质层新生血管极少；RT-PCR检测结果显示，治疗组PEDF mRNA表达明显高于对照组和空白组，差异均有显著统计学意义（均为P＜0.01）；ELISA检测结果显示，治疗组房水中TNF-α蛋白含量明显低于对照组与空白组，差异均有显著统计学意义（均为P＜0.01）。结论 1.6×10-6 H2水滴眼能有效抑制大鼠碱烧伤诱导的CNV。
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| 关 键 词： | 氢气水 角膜新生血管 色素上皮衍生因子 肿瘤坏死因子-α |
Inhibitory effects of hydrogen on corneal neovascularization in rats with alkali burn |
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| WEN Shu,YANG Wei.Inhibitory effects of hydrogen on corneal neovascularization in rats with alkali burn[J].Recent Advances in Ophthalmology,2018,0(1):039-43. |
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| Authors: | WEN Shu YANG Wei |
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| Affiliation: | Department of Ophthalmology，the First Hospital Affiliated to Shihezi University，Shihezi 832000，Xinjiang Uygur Autonomous Region，China |
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| Abstract: | Objective To observe the inhibitory effects of hydrogen on corneal neovascularization (CNV) induced by alkali burn in rats and its underlying mechanisms Methods A total of 48 SPF-grade SD rats (48 eyes) were selected and randomly divided into blank group，control group and treatment group.Then the model of CNV was established successfully induced by alkali burn in these animals.The treatment group was given 1.6×10-6 hydrogen eye drops，the control group received tobramycin dexamethasone eye drops，and blank group left untreated.And CNV length was measured on day 1，3，7 and 14 after model establishment，followed by calculation of the CNV area.The corneal and aqueous humor of the rats was removed on day 14 after alkali burn.Then PEDF protein was detected by immunohistochemical staining，and RT-PCR was used to detect the expression of PEDF mRNA in the cornea.Additionally，TNF-α in the aqueous humor was detected by enzyme e-linked immunosorbent assay (ELISA).Results The area of corneal neovascularization on day 3，7 and 14 after alkali burn was (3.26±1.82)mm2，(8.59±3.98)mm2 and (3.24±2.34)mm2 in the treatment group，respectively，(4.27±2.68)mm2，(6.44±4.67)mm2 and (15.98±4.75)mm2 in the control group，respectively，(9.49±2.79)mm2，(17.71±4.06)mm2 and (25.35±1.40)mm2 in blank group，respectively.The CNV areas of the treatment group and the control group were less than that of the blank group on day 3，7 and 14 after alkali burn，and the differences were statistically significant (all P<0.01)，but there was no significant difference between the treatment group and the control group on day 3，7 after alkali burn (P>0.05)，while the area of the treatment group was significantly less than that of the control group on 14th day，and the difference was statistically significant (P<0.01).On day 14 after alkali burn，the results of immunohistochemistry showed that the expression of PEDF protein in corneal epithelium was significantly increased in the treatment group than that in the control group and the blank group，and there were few new blood vessels in the stromal layer.RT-PCR results showed that the expression of PEDF mRNA in the treatment group was significantly higher than that in the control group and the blank group，and the difference was statistically significant (all P<0.01).ELISA results showed that the content of TNF-α protein in the treatment group was significantly lower than that in the control group and the blank group，and the difference was statistically significant (all P<0.01).Conclusion 1.6×10-6 hydrogen eye drops can effectively inhibit the corneal neovascularization which induced by alkali burn in rats. |
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| Keywords: | hydrogen corneal neovascularization pigment epithelium-derived factor tumor necrosis factor-α |
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