| 食管鳞状细胞癌HPV 16/18型感染与p53表达的相关性研究 |
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| 引用本文： | 詹泳杰,吴腾飞,茅乃权.食管鳞状细胞癌HPV 16/18型感染与p53表达的相关性研究[J].中国癌症防治杂志,2018,10(6):444-448. |
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| 作者姓名： | 詹泳杰 吴腾飞 茅乃权 |
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| 作者单位： | 广西医科大学附属肿瘤医院胸外科 |
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| 基金项目： | 广西壮族自治区卫生厅重点课题资助项目（重 2012089） |
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| 摘 要： | 目的 探讨人乳头状瘤病毒（HPV）16/18型在食管鳞状细胞癌中的感染情况及其与p53的关系。方法 采用聚合酶链式反应（polymerase chain reaction，PCR）检测56例食管鳞状细胞癌手术切除组织（鳞癌组）和24名健康者正常食管组织（对照组）中HPV l6/18 E6、E7基因的表达，并根据其判断HPV感染情况；采用免疫组化SP法检测p53蛋白的表达水平。 结果 鳞癌组HPV感染率和p53蛋白阳性表达率均高于对照组（44.6% vs 12.5%，χ2=7.630，P=0.006；41.1% vs 4.2%，χ2=10.896，P<0.001）。在食管鳞癌组织中，HPV 16/18感染与病理分级、TNM分期和淋巴结转移有关（P<0.05）；且HPV16/18感染与p53蛋白表达呈正相关（r=0.565，P<0.001）。Kaplan-Meier生存分析结果显示，HPV感染阳性、p53表达阳性患者中位OS均小于HPV感染阴性、p53表达阴性者（21个月 vs 39个月，χ2=4.306，P=0.038；17个月 vs 41个月，χ2=5.868， P=0.015）。控制相关的潜在混杂因素后，Cox回归模型显示HPV感染（HR=1.834，95%CI：1.010~3.330，P=0.046）和p53阳性表达（HR=2.189，95%CI：1.182~4.054， P=0.013）均可增加食管鳞状细胞癌患者的死亡风险。结论 食管鳞状细胞癌中HPV感染和p53阳性表达均较高，可能共同促进食管鳞状细胞癌的发生、发展并影响其预后。
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Correlation between HPV 16/18 infection and p53 expression in esophageal squamous cell carcinoma |
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| Zhan Yongjie,Wu Tengfei,Mao Naiquan.Correlation between HPV 16/18 infection and p53 expression in esophageal squamous cell carcinoma[J].Chinese Journal of Oncology Prevention and Treatment,2018,10(6):444-448. |
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| Authors: | Zhan Yongjie Wu Tengfei Mao Naiquan |
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| Abstract: | Objective To investigate the infection of HPV16/18 in esophageal squamous cell carcinoma （ESCC） and its relationship with p53 mutation. Methods PCR was used to detect HPV16/18 E6、E7 genes in 56 cases of ESCC tissues and 24 cases of normal esophageal tissues； immunohistochemical technique（SP） was used to detect the protein of p53. Results In total，the infection rates of HPV16/18 in ESCC tissue and normal tissue were 44.6% and 12.5%，respectively，and the positive expression rates of p53 were 41.1% and 4.2%，respectively. In ESCC tissue，patients displayed higher infection rate of HPV16/18 and positive expression rate of p53 as compared to those in the normal tissue（χ2=7.630，P=0.006； χ2=10.896，P<0.001）. Clinicopathological analysis demonstrated that the HPV16/18 infection in the ESCC tissue was associated with histopathologic grading，TNM stage and lymph node metastasis（P<0.05）. Additionally， HPV16/18 infection rate increased in parallel with p53 positive expression rate （r=0.565，P<0.001）. Kaplan-Meier survival analysis showed that the median OS of patients with positive HPV infection and positive p53 expression were less than those with negative HPV infection and negative for p53 expression（21 months vs 39 months，χ2=4.306，P=0.038；17 months vs 41 months，χ2=5.868，P=0.015）. After adjustment for potential confounders，Multivariate Cox regression showed that HPV infection （HR=1.834，95% CI：1.010-3.330，P=0.046） and p53 positive expression （HR=2.189，95% CI：1.182-4.054，P=0.013） could increase the risk of death in patients with ESCC. Conclusion HPV infection and p53 positive expression were both higher in ESCC，which may jointly promote the occurrence and development of ESCC and affect its prognosis. |
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| Keywords: | Esophageal squamous cell carcinoma HPV16/18 p53 PCR Immunohistochemistry |
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