| 蚕蛹抗氧化肽的制备及其体外抗氧化活性评价 |
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| 引用本文： | 卢楠,廖鲜艳,翁新楚,宋红生.蚕蛹抗氧化肽的制备及其体外抗氧化活性评价[J].上海大学学报(自然科学版),2013,19(2):215-220. |
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| 作者姓名： | 卢楠 廖鲜艳 翁新楚 宋红生 |
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| 作者单位： | 上海大学生命科学学院, 上海200444 |
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| 基金项目： | 上海市教委重点学科建设资助项目(J50108); 上海高校选拔培养优秀青年教师科研专项基金资助项目(SHU09063) |
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| 摘 要： | 为更客观地评价蚕蛹抗氧化肽的抗氧化能力, 以人工胃肠模拟液对不同蛋白酶酶解物的抗氧化能力进行评价, 优选获得最佳的制备用酶——碱性蛋白酶. 在此基础上,以蚕蛹抗氧化肽总还原能力为指标, 利用响应曲面法(response surface method, RSM)优化碱性蛋白酶制备蚕蛹抗氧化肽的最佳酶解工艺. 结果表明: 碱性蛋白酶的最佳酶解条件为pH=8.30, 加酶量为15.34 μL(1 902 U), 温度为50.47 ℃, 时间为2.45 h; 在此条件下优化预测得到的2 mg 蚕蛹蛋白/mL 酶解液的总还原能力为0.434, 蚕蛹抗氧化肽对·OH 和DPPH· 自由基的半数清除率IC50 分别为4.51 与4.24 mg/mL.
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| 关 键 词： | 蚕蛹蛋白 抗氧化肽 酶解 响应曲面法 |
| 收稿时间： | 2012-02-20 |
Preparation of Antioxidant Peptides from Silkworm Pupae Protein and Evaluation of Activities in vitro |
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| LU Nan,LIAO Xian-yan,WENG Xin-chu,SONG Hong-sheng.Preparation of Antioxidant Peptides from Silkworm Pupae Protein and Evaluation of Activities in vitro[J].Journal of Shanghai University(Natural Science),2013,19(2):215-220. |
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| Authors: | LU Nan LIAO Xian-yan WENG Xin-chu SONG Hong-sheng |
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| Affiliation: | School of Life Sciences, Shanghai University, Shanghai 200444, China |
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| Abstract: | Alkaline protease was chosen by the effect of gastrointestinal fluid simulation which was used to predict the activity in vivo of silkworm pupae protein. Response surface method (RSM) was employed to optimum the optimal alkaline protease enzymolysis procedure of silkworm pupae protein for antioxidant peptides with high reducing power. Antioxidant activity of enzymolysates was assessed with reducing power and scavenging effects against ·OH and DPPH·radicals. Results show that the optimum conditions are as follows: pH at 8.30, quantity of alkaline protease at 15.34 μL(1 902 U), temperature at 50.47 ℃ and time at 2.45 h. Under the optimum conditions, the predicted total reducing power of 2.0 mg silkworm pupae protein/mL enzymolysates was 0.434. On the basis of the optimum conditions of the enzymolysates, the half-scavenging concentration IC50 values of ·OH and DPPH·radicals were 4.51 and 4.24 mg/mL respectively. |
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| Keywords: | antioxidant peptides enzymolysis response surface method (RSM) silkworm pupae protein |
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