| 白藜芦醇改善高糖引起肾小球系膜细胞损伤的作用研究 |
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| 引用本文： | 王琳,刘亚珊,刘瑞卿,高爱娇,唐慧欣,申艳娜.白藜芦醇改善高糖引起肾小球系膜细胞损伤的作用研究[J].天津医科大学学报,2021,0(4):338-342. |
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| 作者姓名： | 王琳 刘亚珊 刘瑞卿 高爱娇 唐慧欣 申艳娜 |
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| 作者单位： | （天津医科大学医学检验学院,天津300203） |
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| 摘 要： | 目的:探讨白藜芦醇（RSV）对体外高糖刺激引起大鼠肾小球系膜细胞（RGMCs）损伤的影响及其潜在分子保护机制。方法:取处于对数生长期的RGMCs，分为甘露醇高渗透压对照组（OC）、正常对照组（NG）、高糖组（HG）以及高糖RSV处理组，分别处理24、48 h。试剂盒检测不同时间各组细胞上清液中LDH的水平；Western 印迹法检测不同时间各组凋亡相关B淋巴细胞瘤-2基因（Bcl-2）、Bcl-2相关X蛋白（Bax）、半胱氨酸天冬氨酸蛋白酶（Caspase）-1、Caspase-1活性片段（p20）、Gasdermin E（GSDME）、GSDME活性片段（GSDME-N）、Caspase-3、Caspase-3活性片段（p17）、炎性相关细胞因子白细胞介素-1β（IL-1β）及其活化形式（mIL-1β）的表达；ELISA检测不同时间各组细胞培养上清IL-1β的水平。结果:LDH结果显示，RSV可降低高糖刺激48 h后细胞的死亡率（F=31.48，P<0.05）。Western 印迹法结果显示，仅在RSV处理24 h后，Bax表达较HG组显著降低，同时伴随Bcl-2表达升高（F=143.1，P<0.05）；RSV处理24 h后HG组p20、mIL-1β表达降低（F=26.74、25.76，均P<0.05）；此外，RSV处理48 h后p17、GSDME-N的表达较HG组明显下降（F=25.84、18.49，均P<0.05）。ELISA结果显示,与HG组相比，RSV处理48 h后上清中IL-1β的水平显著降低（F=80.22，P<0.01）。上述指标在OC组及NG组间的变化均无统计学意义（均P＞0.05）。结论:RSV可通过减少炎症因子的产生及抑制细胞早期凋亡、晚期焦亡过程来改善高糖引起的RGMCs损伤。
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| 关 键 词： | 白藜芦醇 糖尿病肾病 细胞死亡 GSDME |
Effect of resveratrol on glomerular mesangial cell injury induced by high glucose |
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| WANG Lin,LIU Ya-shan,LIU Rui-qing,GAO Ai-jiao,TANG Hui-xin,SHEN Yan-na.Effect of resveratrol on glomerular mesangial cell injury induced by high glucose[J].Journal of Tianjin Medical University,2021,0(4):338-342. |
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| Authors: | WANG Lin LIU Ya-shan LIU Rui-qing GAO Ai-jiao TANG Hui-xin SHEN Yan-na |
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| Affiliation: | （School of Medical Laboratory，Tianjin Medical University，Tianjin 300203，China ） |
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| Abstract: | Objective: To investigate the effect of resveratrol（RSV） on the injury of rat glomerular mesangial cells（RGMCs） induced by high glucose in vitro and its potential molecular protection mechanism. Methods: RGMCs in logarithmic growth phase were divided into mannitol hypertonic control group（OC），normal control group（NG），high glucose group（HG） and high glucose with RSV treatment group,and RGMCs were cultured for 24 h and 48 h respectively. The levels of LDH in the supernatant of each group were detected by the LDH release assay kit. The protein levels of apoptosis related B-cell lymphoma-2 gene（Bcl-2），Bcl-2 associated X protein（Bax），cysteine-containing aspartate specific protease 1（Caspase-1），Caspase-1 activated fragment（p20），Gasdermin E（GSDME），Gasdermin E activated fragment（GSDME-N），Caspase-3，Caspase-3 activated fragment（p17），inflammatory related cytokines interleukin-1β（IL-1β） and mature form of IL-1β（mIL-1β） were detected by Western blotting analysis.The levels of IL-1β in the supernatant were detected by ELISA analysis. Results: LDH assay showed that RSV reduced the cell death rate after 48 h of high glucose stimulation（F=31.48，P<0.05）. Western blotting analysis showed that the expression of Bax in RSV treatment group was significantly lower than that in HG group only at 24 h and the expression of Bcl-2 was increased（F=143.1，P<0.05）. RSV also decreased the expression of p20 and mIL-1β in HG group at 24 h（F=26.74，25.76，all P<0.05）.Moreover，RSV significantly decreased the expression of p17 and GSDME-N in HG group at 48 h（F=25.84，18.49，all P<0.05）. ELISA analysis showed that IL-1β level in the supernatant of RGMCs with RSV treatment for 48 h was significantly reduced than that in HG group（F=80.22，P<0.01）. There were no significant difference in above related indexes between OC group and NG group （all P＞0.05）. Conclusion: RSV can improve the damage in RGMCs induced by high glucose via reducing the production of inflammatory factors，inhibiting the early apoptotic and late pyroptosis. |
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| Keywords: | resveratrol diabetic nephropathy cell death GSDME |
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